Petri dish PCR: laser-heated reactions in nanoliter droplet arrays

Kim, Hanyoup; Vishniakou, Siarhei; Faris, Gregory W.

doi:10.1039/b817288a

Cited by 75 publications

(61 citation statements)

References 33 publications

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“…33,36 In addition, such data are critical to applications in which the droplet temperatures control physical processes other than evaporation, such as voltage-induced modulation of droplet temperatures for biosensing, 37 control of reaction rates in droplet microfluidics, 38,39 and temperature modulation to denature DNA for polymerase chain reactions. 40,41 The methodology is also broadly applicable for characterizing the temperature of curved interfaces.…”

Section: -2mentioning

confidence: 99%

Spatiotemporal infrared measurement of interface temperatures during water droplet evaporation on a nonwetting substrate

Chandramohan

Weibel

Garimella

2017

Applied Physics Letters

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High-fidelity experimental characterization of sessile droplet evaporation is required to understand the interdependent physical mechanisms that drive the evaporation. In particular, cooling of the interface due to release of the latent heat of evaporation, which is not accounted for in simplified vapor-diffusion-based models of droplet evaporation, may significantly suppress the evaporation rate on nonwetting substrates, which support tall droplet shapes. This suppression is counteracted by convective mass transfer from the droplet to the air. While prior numerical modeling studies have identified the importance of these mechanisms, there is no direct experimental evidence of their influence on the interfacial temperature distribution. Infrared thermography is used here to simultaneously measure the droplet volume, contact angle, and spatially resolved interface temperatures for water droplets on a nonwetting substrate. The technique is calibrated and validated to quantify the temperature measurement accuracy; a correction is employed to account for reflections from the surroundings when imaging the evaporating droplets. Spatiotemporally resolved interface temperature data, obtained via infrared thermography measurements, allow for an improved prediction of the evaporation rate and can be utilized to monitor temperature-controlled processes in droplets for various lab-on-a-chip applications.

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Section: -2mentioning

confidence: 99%

Spatiotemporal infrared measurement of interface temperatures during water droplet evaporation on a nonwetting substrate

Chandramohan

Weibel

Garimella

2017

Applied Physics Letters

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“…In addition, laser-induced heating has also been utilized to assist biological process [25], which benefits from a small heating region controlled by laser spot. The precise temperature detection and calibration rely on microsensor [26,27] or optical imaging technique [28]. Although many studies have focused on the laser-induced heating effect, the effect occurring in the solid and fluid flow, a more complicated problem, was found in our previous work.…”

Section: Introductionmentioning

confidence: 95%

Effect of Laser-Induced Heating on Raman Measurement within a Silicon Microfluidic Channel

Lin

Wang

et al. 2015

Micromachines

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When Raman microscopy is adopted to detect the chemical and biological processes in the silicon microfluidic channel, the laser-induced heating effect will cause a temperature rise in the sample liquid. This undesired temperature rise will mislead the Raman measurement during the temperature-influencing processes. In this paper, computational fluid dynamics simulations were conducted to evaluate the maximum local temperature-rise (MLT). Through the orthogonal analysis, the sensitivity of potential influencing parameters to the MLT was determined. In addition, it was found from transient simulations that it is reasonable to assume the actual measurement to be steady-state. Simulation results were qualitatively validated by experimental data from the Raman measurement of diffusion, a temperature-dependent process. A correlation was proposed for the first time to estimate the MLT. Simple in form and convenient for calculation, this correlation can be efficiently applied to Raman measurement in a silicon microfluidic channel.

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“…Because fast/ultrafast PCR is highly desirable for applications such as timely diagnosis of infectious diseases, cardiac diseases, cancer, neurological disorder diseases, and rapid biowarfare and pathogen identification at the point-of-care (POC) level, many academic and industrial groups have worked on improving PCR systems, [9][10][11][12][13][14][15] One commercial PCR system (LightCycler H 2.0, Roche Diagnostics USA, Indianapolis, IN, USA) using air heating/cooling and capillary tubes can perform 30 thermal cycles in 10-60 min, depending on sample volume. 10 However, this system is not suitable for POC testing due to its high power consumption (800 W maximum) and heavy weight (approximately 22 kg).…”

Section: Introductionmentioning

confidence: 99%

“…Another approach includes infrared-mediated non-contact selective heating of water droplets (nanoliter sample volume) for ultrafast thermal cycling using an infrared laser, which harnesses the strong absorbance of water at wavelengths over 1000 nm. 14,15 However, droplet formation from the PCR mixture is a precise process prone to human error, which is a drawback for POC testing.…”

Section: Introductionmentioning

confidence: 99%

Ultrafast photonic PCR

et al. 2015

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Nucleic acid amplification and quantification via polymerase chain reaction (PCR) is one of the most sensitive and powerful tools for clinical laboratories, precision medicine, personalized medicine, agricultural science, forensic science and environmental science. Ultrafast multiplex PCR, characterized by low power consumption, compact size and simple operation, is ideal for timely diagnosis at the point-of-care (POC). Although several fast/ultrafast PCR methods have been proposed, the use of a simple and robust PCR thermal cycler remains challenging for POC testing. Here, we present an ultrafast photonic PCR method using plasmonic photothermal light-to-heat conversion via photon-electron-phonon coupling. We demonstrate an efficient photonic heat converter using a thin gold (Au) film due to its plasmon-assisted high optical absorption (approximately 65% at 450 nm, the peak wavelength of heat source light-emitting diodes (LEDs)). The plasmon-excited Au film is capable of rapidly heating the surrounding solution to over 150 6C within 3 min. Using this method, ultrafast thermal cycling (30 cycles; heating and cooling rate of 12.7960.93 6C s 21 and 6.660.29 6C s 21 , respectively) from 55 6C (temperature of annealing) to 95 6C (temperature of denaturation) is accomplished within 5 min. Using photonic PCR thermal cycles, we demonstrate here successful nucleic acid (l-DNA) amplification. Our simple, robust and low cost approach to ultrafast PCR using an efficient photonic-based heating procedure could be generally integrated into a variety of devices or procedures, including on-chip thermal lysis and heating for isothermal amplifications.

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Petri dish PCR: laser-heated reactions in nanoliter droplet arrays

Cited by 75 publications

References 33 publications

Spatiotemporal infrared measurement of interface temperatures during water droplet evaporation on a nonwetting substrate

Spatiotemporal infrared measurement of interface temperatures during water droplet evaporation on a nonwetting substrate

Effect of Laser-Induced Heating on Raman Measurement within a Silicon Microfluidic Channel

Ultrafast photonic PCR

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