Petrimonas sulfuriphila gen. nov., sp. nov., a mesophilic fermentative bacterium isolated from a biodegraded oil reservoir

Abstract

A mesophilic, anaerobic, fermentative bacterium, strain BN3T, was isolated from a producing well of a biodegraded oil reservoir in Canada. Cells were Gram-negative, non-motile rods that did not form spores. The temperature range for growth was 15–40 °C, with optimum growth at 37–40 °C. The strain grew with up 4 % NaCl, with optimum growth in the absence of NaCl. Tryptone was required for growth. Yeast extract and elemental sulfur stimulated growth. Growth was also enhanced during fermentation of glucose, arabinose, galactose, maltose, mannose, rhamnose, lactose, ribose, fructose, sucrose, cellobiose, lactate, mannitol and glycerol. Acetate, hydrogen and CO2were produced during glucose fermentation. Elemental sulfur and nitrate were used as electron acceptors and were reduced to sulfide and ammonium, respectively. The G+C content of the genomic DNA was 40·8 mol%. Phylogenetic analyses of the 16S rRNA gene sequence indicated that the strain was a member of the phylum ‘Bacteroidetes’, distantly related to the generaBacteroidesandTannerella(similarity values of less than 90 %). The chemotaxonomic data (fatty acids, polar lipids and quinones composition) also indicated that strain BN3Tcould be clearly distinguished from its closest cultivated relatives. This novel organism possesses phenotypic, chemotaxonomic and phylogenetic traits that do not allow its classification as a member of any previously described genus; therefore, it is proposed that this isolate should be described as a member of a novel species of a new genus,Petrimonasgen. nov., of whichPetrimonas sulfuriphilasp. nov. is the type species. The type strain is BN3T(=DSM 16547T=JCM 12565T).

Unexpected Stability of Bacteroidetes and Firmicutes Communities in Laboratory Biogas Reactors Fed with Different Defined Substrates

“…6C). P. sulfuriphila, which was related to OTU 1, is a strictly anaerobic bacterium that ferments carbohydrates and organic acids (11). Many bacteria identified as being closely related to Bacteroidetes OTUs from this study, like Proteiniphilum acetatigenes, P. sulfuriphila, and A. imshenetskii, were also found previously in other biogas reactors (18,19).…”

Description of Proteiniphilum saccharofermentans sp. nov., Petrimonas mucosa sp. nov. and Fermentimonas caenicola gen. nov., sp. nov., isolated from mesophilic laboratory-scale biogas reactors, and emended description of the genus Proteiniphilum

“…Strain ING2-E5B T showed weak growth on both peptone and tryptone and on a mixture of the two. A requirement for tryptone, as described for Petrimonas sulfuriphila BN3 T (Grabowski et al, 2005), was not observed for the novel strains. For Proteiniphilum acetatigenes TB107 T , it was reported that the strain was not able to use any of a variety of tested carbohydrates (Chen & Dong, 2005); however, in our experiments, all tested mono-and disaccharides (arabinose, cellobiose, fructose, galactose, glucose, lactose, maltose, mannose, rhamnose, sucrose and trehalose) supported cell growth of Proteiniphilum acetatigenes DSM 18083…”

Prokaryotic Communities in Pit Mud from Different-Aged Cellars Used for the Production of Chinese Strong-Flavored Liquor

“…The Lactobacillus species produce lactic acid as the major end product in carbohydrate fermentation (28). Petrimonas is reported to produce acetic acid, hydrogen, and CO 2 during glucose fermentation (29). Sedimentibacter ferments amino acids and glycine to ethanol or to acetic acid and butyric acid (30,31).…”