Petroselinum sativum protects HepG2 cells from cytotoxicity and oxidative stress induced by hydrogen peroxide

Al-Oqail, Mai M.; Farshori, Nida Nayyar; Al-Sheddi, Ebtesam S.; Al-Massarani, Shaza M.; Siddiqui, Maqsood A.; Al‐Khedhairy, Abdulaziz A.

doi:10.1007/s11033-020-05380-z

Cited by 15 publications

(10 citation statements)

References 44 publications

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“…Through specific hydrolysis, it activates procaspases 2, 6, 7, and 9, which causes the cleavage of several important apoptotic proteins like PARP. According to studies, the mechanism underlying the induction of cellular apoptosis in HepG 2 cells by oxidative damage involves a reduction in the activity of antioxidant enzymes, leads to oxidative injury and increases the activity of caspase-9 and caspase-3 proteases ( 20 , 24 , 25 ). Analysis of Figure 9 reveals that under the influence of H 2 O 2 , there is a significant augmentation ( p < 0.05) in the activity of caspase-8, 9, and 3, which can induce cellular apoptosis.…”

Section: Resultsmentioning

confidence: 99%

The antioxidant activity of polysaccharides from Armillaria gallica

Su,

Qiu,

Liang

et al. 2024

Front. Nutr.

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The purpose of this study was to investigate the antioxidant activity of Armillaria gallica polysaccharides. It explored whether Armillaria gallica polysaccharides (AgP) could prevent HepG2 cells from H2O2-induced oxidative damage. The results demonstrated that HepG2 cells were significantly protected by AgP, and efficiently suppressed the production of reactive oxygen species (ROS) in HepG2 cells. Additionally, AgP significantly decreased the abnormal leakage of alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) caused by H2O2, protecting cell membrane integrity. It was discovered that AgP was also found to regulate the activities of antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-PX), while reducing malondialdehyde (MDA), thus protecting cells from oxidative damage. According to the flow cytometry analysis and measurement of caspase-3, caspase-8, and caspase-9 activities, AgP could modulate apoptosis-related proteins and attenuate ROS-mediated cell apoptosis.

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Section: Resultsmentioning

confidence: 99%

The antioxidant activity of polysaccharides from Armillaria gallica

Su,

Qiu,

Liang

et al. 2024

Front. Nutr.

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“…In other study, the cytotoxic/antiprolifertaive potential of ethyl acetate extract against HT-29 human colon cancer and MCF-7 human breast cancer cells have also been reported (Lansky and Newman, 2007). The anticancer potential of chloroform extract and sub-fractions of Nepeta deflersiana against MCF-7 human breast cancer and A549 human lung carcinoma cell lines have been studied (Al-Oqail et al, 2015). Recently, Usmani et al, (2018) have also reported the antiproliferative and cellular oxidative stress induced by Cordia dichotoma (Linn.)…”

Section: Discussionmentioning

confidence: 98%

Cytotoxic Potential of Petroleum ether, Ethyl Acetate, Chloroform, and Ethanol Extracts of Lavandula Coronopifolia Against Human Breast Carcinoma Cell line (MDA-MB-321)

Al-Sheddi

2019

Asian Pac J Cancer Prev

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Background: Breast cancer is the most common cause of deaths in women. The search for traditionally used medicinal plants which can serve as non-toxic and affordable anticancer drugs is the need of the hour. This study aimed to investigate the anticancer potential of extracts of L. coronopifolia against human breast carcinoma cell line (MDA-MB-321). Methods: The MDA-MB-231 cells were plated in 96 well plates and exposed to 10-1,000 µg/ml of L. coronopifolia for 24 h. The cytotoxic response of different extracts was measured by MTT assay, neutral red uptake (NRU) assay and cellular morphological alterations under the microscope. Results: A concentration-dependent decrease in the cell viability of MDA-MB-231 cells was observed after the exposure of petroleum ether, ethyl acetate, chloroform, and ethanol extracts of L. coronopifolia. The cell viability was found to be 82%, 89% and 98% at 1000, 500 and 250 µg/ml, respectively in petroleum ether, 37%, 75% and 88% at 1,000, 500 and 250 µg/ml, respectively in ethyl acetate extract, 30%, 35% and 64% at 1,000, 500 and 250 µg/ml, respectively in chloroform extract and 44%, 65% and 82% at 1000, 500 and 250 µg/ml, respectively in ethanolic extract of L. coronopifolia exposed MDA-MB-231 cells. The results also exhibited morphological alterations in MDA-MB-231 cells exposed to various extracts. The cells treated with 250-1000 µg/ml lost their original morphology and cell linkage as compared to control cells. Conclusion: These preliminary results suggest the promising anticancer potential of petroleum ether, ethyl acetate, chloroform, and ethanol extracts of L. coronopifolia against MDA-MB-321 cells. Further studies are required to know the mechanism(s) involved in the cell death.

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“…The liver cancer cell line HepG2 has been widely used in evaluating the hepatoprotective activities of novel agents ( 48 ), as these cells retain several morphological and biochemical characteristics of normal hepatocytes ( 49 ). In the present study, the possible cytotoxic effects of BB on HepG2 cells were firstly excluded.…”

Section: Discussionmentioning

confidence: 99%

Bilobalide attenuates lipopolysaccharide‑induced HepG2 cell injury by inhibiting TLR4‑NF‑κB signaling via the PI3K/Akt pathway

Mao,

Yao,

Zhang

et al. 2023

Exp Ther Med

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Inflammation is involved in the pathological process underlying a number of liver diseases. Bilobalide (BB) is a natural compound from Ginkgo biloba leaves that was recently demonstrated to exert hepatoprotective effects by inhibiting oxidative stress in the liver cancer cell line HepG2. The anti-inflammatory activity of BB has been reported in recent studies. The major objective of the present study was to investigate whether BB could attenuate inflammation-associated cell damage. HepG2 cells were cultured with lipopolysaccharide (LPS) and BB, and cell damage was evaluated by measuring cell viability using MTT assay. The activity of the NF-κB signaling pathway was assessed by measuring the levels of IκBα, NF-κB p65, phosphorylated (p)-IκBα, p-p65, p65 DNA-binding activity and inflammatory cytokines IL-1β, IL-6 and TNF-α. A toll-like receptor (TLR)4 inhibitor (CLI-095) was used to detect the involvement of TLR4 in cell injury caused by LPS. In addition, the PI3K/Akt inhibitor LY294002 was applied to explore the involvement of the PI3K/Akt axis in mediating the effects of BB. The results demonstrated that LPS induced HepG2 cell injury. LPS also elevated the levels of p-IκBα, p-p65, p65 DNA-binding activity and inflammatory cytokines. However, CLI-095 significantly attenuated the LPS-induced cell damage and inhibited the activation of NF-κB signaling. BB also dose-dependently attenuated the LPS-induced cell damage, activation of NF-κB signaling and TLR4 overexpression. Furthermore, it was observed that LY294002 diminished the cytoprotective effects of BB on cell injury, TLR4 expression and NF-κB activation. These findings indicated that BB could attenuate LPS-induced inflammatory injury to HepG2 cells by regulating TLR4-NF-κB signaling.

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Petroselinum sativum protects HepG2 cells from cytotoxicity and oxidative stress induced by hydrogen peroxide

Cited by 15 publications

References 44 publications

The antioxidant activity of polysaccharides from Armillaria gallica

The antioxidant activity of polysaccharides from Armillaria gallica

Cytotoxic Potential of Petroleum ether, Ethyl Acetate, Chloroform, and Ethanol Extracts of Lavandula Coronopifolia Against Human Breast Carcinoma Cell line (MDA-MB-321)

Bilobalide attenuates lipopolysaccharide‑induced HepG2 cell injury by inhibiting TLR4‑NF‑κB signaling via the PI3K/Akt pathway

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