26Upon entering building plumbing systems, drinking water bacteria experience 27 considerable changes in environmental conditions. For example, some flexible 28 polymeric materials leach organic carbon, which increases bacterial growth and 29 reduces diversity. Here we show that the carbon supply by a flexible polymeric material 30 drives nutrient-based selection within establishing biofilm communities. We found that 31 migrating carbon from EPDM coupons resulted in considerable growth for different 32 drinking water communities (0.2 -3.3 x 10 8 cells/cm 2 ). All established biofilm 33 communities showed low diversity (29 -50 taxa/biofilm), with communities dominated 34 by even viewer taxa (e.g., 5 taxa accounting for 94 ± 5 % relative abundance, n = 15). 35Interestingly, biofilm communities shared some taxa (e.g., Methylobacterium spp.) and 36 families (e.g., Comamonadaceae), despite the difference in starting communities. 37Moreover, selected biofilm communities performed better than their original 38 communities regarding maximum attachment (91 ± 5 vs. 69 ± 23 %, n = 15) and 39 attachment rate (5.0 ± 1.7 x 10 4 vs. 2.4 ± 1.2 x 10 4 cells/cm 2 /h, n = 15) when exposed 40 to new EPDM coupons. Our results demonstrate nutrient-based selection during initial 41 biofilm formation on a flexible polymeric material and a resulting benefit to selected 42 communities. We anticipate our findings to help connecting observational 43 microbiological findings with their underlying ecological principles. Regarding initial 44 biofilm formation, attachment dynamics, growth, and selection thereof are important 45 for the management of microbial communities. In fact, managing initial colonization by 46 supplying specific carbon and/or introducing consciously chosen/designed 47 communities potentially paves the way for a probiotic approach for building plumbing 48 materials. 49 50