In a recent study, Liang et al. (2017, https://doi.org/10.1002/2017JA024127) repeatedly identified strong electron temperature (T e ) enhancements when Swarm satellites traversed pulsating auroral patches. In this study, we use joint optical and Poker Flat Incoherent Scatter Radar (PFISR) observations to further investigate the F region plasma signatures related to pulsating auroras. On 19 March 2015 night, which contained multiple intervals of pulsating auroral activities, we identify a statistical trend, albeit not a one-to-one correspondence, of strong T e enhancements (~500-1000 K) in the upper F region ionosphere during the passages of pulsating auroras over PFISR. On the other hand, there is no discernible and repeatable density enhancement in the upper F region during pulsating auroral intervals. Collocated optical and NOAA satellite observations suggest that the pulsating auroras are composed of energetic electron precipitation with characteristic energy >10 keV, which is inefficient in electron heating in the upper F region. Based upon PFISR observations and simulations from Liang et al. (2017) model, we propose that thermal conduction from the topside ionosphere, which is heated by precipitating low-energy electrons, offers the most likely explanation for the observed electron heating in the upper F region associated with pulsating auroras. Such a heating mechanism is similar to that underlying the "stable auroral red arcs" in the subauroral ionosphere. Our proposal conforms to the notion on the coexistence of an enhanced cold plasma population and the energetic electron precipitation, in magnetospheric flux tubes threading the pulsating auroral patch. In addition, we find a trend of enhanced ion upflows during pulsating auroral intervals.On the other hand, if the downgoing magnetospheric electrons include a component of substantially "colder" electrons, for example, on order of~10 eV or less, electron heating in the upper ionosphere may operate in a fundamentally different way. The "cold" magnetospheric electrons cannot penetrate deeply into the ionosphere, but can strongly heat electrons near the topside boundary of the ionosphere. The heat will be conducted toward lower altitudes into the F region ionosphere. In this scenario, the T e enhancement seen in the F region ionosphere is a remote effect, meaning that the low-energy magnetospheric electrons do not actually penetrate to F region altitudes, so no significant ionization or red-line auroral excitation is LIANG ET AL. 4430