PGE2 exerts dose-dependent opposite effects on net water and chloride absorption from the rat colon

Kreydiyyeh, Sawsan Ibrahim; Markossian, Sarine; Hodeify, Rawad

doi:10.1016/j.prostaglandins.2005.07.004

Cited by 5 publications

(2 citation statements)

References 33 publications

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“…The single-pass intestinal in situ perfusion method is commonly used either to investigate water and electrolyte absorption in the intestine or to determine the intestinal mechanism of the absorption of drugs (Salphati et al, 2001; Cook and Shenoy, 2003; Kreydiyyeh et al, 2006; Nagare et al, 2010). It has also been applied in experimental IBD models for studying the impact of barrier and transport function, as well as the inhibitory effect on fluid absorption (Stein et al, 1998; Mourad et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Establishment of a novel extracorporeal bowel model to study luminal approaches to treat inflammatory bowel disease

Breitrück

Sparmann

Mitzner

et al. 2013

Disease Models &Amp; Mechanisms

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SUMMARYWe have established an extracorporeal bowel model system for the analysis of early events in inflammatory bowel disease (IBD) and therapeutic applications. This model consists of an intestinal segment that is cannulated and perfused in situ, allowing the investigation of cellular responses of apical mucosa cells on luminal applied substances. Short-term treatment with iodoacetamide mimicked experimental intestinal inflammation in IBD, as indicated by histological alterations such as hemorrhage, hyperemia and loss of regular crypt architecture, as well as enhanced expression of cytokines (e.g. IL-6, IL-10 and MCP-1) compared with control segments perfused with media. Perfusion of therapeutic agents (e.g. dexamethasone or Mutaflor) in the small intestine segment significantly reduced the features of early inflammation that are induced by iodoacetamide. Moreover, similar data were obtained for Resormin®, a montmorillonite-illite mixed-layer mineral (smectite), indicating that smectites might be a newly identified therapeutic option for IBD. In summary, this model could provide novel insights into epithelial injury as well as genesis of IBD and, therefore, be useful in testing the therapeutic potential of compounds for IBD therapy.

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Section: Discussionmentioning

confidence: 99%

Establishment of a novel extracorporeal bowel model to study luminal approaches to treat inflammatory bowel disease

Breitrück

Sparmann

Mitzner

et al. 2013

Disease Models &Amp; Mechanisms

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“…However, dietary-derived PGE 2 , and potentially PGF 2α , could have effects on the gut. A 10 nM concentration of PGE 2 is able to activate PGE 2 receptors that regulate water and chloride absorption. − Indeed, the K d of PGE 2 for the EP3 and EP4 isoforms of the PGE 2 receptor is below 1 nM, and the EP3 and EP4 subclasses are both highly expressed in the gut. , The extent to which dietary-derived prostanoids could then reach effective concentrations in the blood for cardiovascular benefit is questionable.…”

Section: Discussionmentioning

confidence: 99%

Baking Reduces Prostaglandin, Resolvin, and Hydroxy-Fatty Acid Content of Farm-Raised Atlantic Salmon (Salmo salar)

Raatz

Golovko

Brose

et al. 2011

J. Agric. Food Chem.

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Consumption of seafood enriched in n-3 polyunsaturated fatty acids (PUFA) is associated with a decreased risk of cardiovascular disease. Several n-3 oxidation products from eicosapentaenoic acid (EPA; 20:5n-3) and docosahexaenoic acid (DHA; 22:6n-3) have known protective effects in the vasculature. It is not known whether consumption of cooked seafood enriched in n-3 PUFA causes appreciable consumption of lipid oxidation products. We tested the hypothesis that baking Atlantic salmon (Salmo salar) increases the level of n-3 and n-6 PUFA oxidation products over raw salmon. We measured the content of several monohydroxy-fatty acids (MHFA), prostanoids, and resolvins. Our data demonstrate that baking did not change the overall total levels of MHFA. However, baking resulted in selective regio-isomeric loss of hydroxy fatty acids from arachidonic acid (20:4n-6), and EPA while significantly increasing hydroxyl-linoleic acid levels. The content of prostanoids and resolvins were reduced several-fold with baking. The inclusion of coating upon the salmon prior to baking reduced the loss of some MHFA but had no effect upon prostanoid losses incurred by baking. Baking did not decrease n-3 PUFA content indicating that baking of salmon is an acceptable means of preparation that does not alter the potential health benefits of high n-3 seafood consumption. The extent to which the levels of MHFA, prostanoids and resolvins in the raw or baked fish have physiologic consequence for humans needs to be determined.

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Interleukin-4 inhibits an early phase in the HIV-1 life cycle in the human colorectal cell line HT-29

Gauthier

Tremblay

2010

Clinical Immunology

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PGE2 exerts dose-dependent opposite effects on net water and chloride absorption from the rat colon

Cited by 5 publications

References 33 publications

Establishment of a novel extracorporeal bowel model to study luminal approaches to treat inflammatory bowel disease

Establishment of a novel extracorporeal bowel model to study luminal approaches to treat inflammatory bowel disease

Baking Reduces Prostaglandin, Resolvin, and Hydroxy-Fatty Acid Content of Farm-Raised Atlantic Salmon (Salmo salar)

Interleukin-4 inhibits an early phase in the HIV-1 life cycle in the human colorectal cell line HT-29

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