PGE2-induced inhibition of AVP-dependent cAMP accumulation in the OMCD of the rat kidney is cumulative with respect to the effects of ?2-adrenergic and A1-adenosine agonists, insensitive to pertussis toxin and dependent on extracellular calcium

Aarab, Lotfi; Montegut, M.; Siaume-Perez, Sylvie; Imbert–Teboul, Martine; Chabardès, Danielle

doi:10.1007/bf00374933

Cited by 17 publications

(22 citation statements)

References 26 publications

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“…The micromethod used allows the determination of 2-80 fmol of cAMP per reaction tube. In our conditions, the basal level of cyclic AMP present in a single piece of tubule is close to, or below, the sensitivity threshold of the assay (20,24 ] e , a concentration previously used in experiments performed on parathyroid cells (3)(4)(5). In each experiment, different experimental conditions were tested on replicate CTAL samples microdissected from a same rat kidney (6 -9 samples per condition).…”

Section: Methodsmentioning

confidence: 99%

“…Isolation of Rat Kidney Cortical Thick Ascending Limbs-The experimental conditions used to perform microdissection of intact segments have been detailed previously (20,24). Experiments were performed on male Sprague-Dawley rats (120 -150 g of body weight, Iffa-Credo, France) that were maintained on a standard diet with free access to water.…”

Section: Methodsmentioning

confidence: 99%

“…The standard microdissection medium was composed of the following (in mM): NaCl, 137; KCl, 5; MgSO 4 , 0.8; Na 2 HPO 4 , 0.33; KH 2 P0 4 , 0.44; MgCl 2 , 1; NaHCO 3 , 4; CH 3 COONa, 10; CaCl 2 , 0.5; glucose, 5; HEPES, 20, pH 7.4, and 0.1% (w/v) bovine serum albumin (fraction V, protease-free, minimal fatty acid content, Pentex, Miles Inc., Kankakee, IL). This medium was supplemented with 10 M ibuprofen and 0.5 unit/ml adenosine deaminase (Boehringer Mannheim) to prevent the synthesis of prostaglandins and the release of adenosine, agents known to interact with the regulation of cAMP content in the rat kidney (20,24). A nominally Ca 2ϩ -free medium used in some of our protocols had the same composition except that calcium was omitted; Ca 2ϩ -chelating agents were not added to this medium because they have been described to modify the sensitivity to Ca 2ϩ of the Ca 2ϩ -inhibitable AC isoforms (25).…”

Section: Methodsmentioning

confidence: 99%

“…Measurement of Hormone-dependent cAMP Accumulation-The experimental conditions used to measure hormone-dependent cAMP accumulation on an intact single segment (20,24) will be recalled briefly. Microdissected pieces of CTAL were transferred in 2 l of incubation medium onto glass slides (1 or 2 pieces per slide) and photographed in order to measure their length.…”

Section: Methodsmentioning

confidence: 99%

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Co-expression of a Ca2+-inhibitable Adenylyl Cyclase and of a Ca2+-sensing Receptor in the Cortical Thick Ascending Limb Cell of the Rat Kidney

Ferreira¹,

Héliès-Toussaint²,

Imbert–Teboul

et al. 1998

Journal of Biological Chemistry

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The Ca 2؉ -sensing receptor protein and the Ca 2؉ -inhibitable type 6 adenylyl cyclase mRNA are present in a defined segment of the rat renal tubule leading to the hypothesis of their possible functional co-expression in a same cell and thus to a possible inhibition of cAMP content by extracellular Ca 2؉ . By using microdissected segments, we compared the properties of regulation of extracellular Ca 2؉ ] e also stimulates phospholipase C activity in parathyroid cells (3, 4) and inhibits hormonedependent cAMP accumulation (5), but, so far, no interaction between these two transduction pathways has been established. The presence of BoPCaR1 in parathyroid cells explains the crucial role of [Ca 2ϩ ] e to elicit a negative feedback on parathyroid hormone secretion (6, 7). In rat kidney, a predominant expression of RaKCaR mRNA has been localized in the cortical portion of the thick ascending limb (8, 9), a segment which ensures cAMP-stimulated paracellular Ca 2ϩ reabsorption, from the lumen of the renal tubule to the extracellular fluid compartments (10). A functional Ca 2ϩ receptor is expressed in the plasma membrane of the rat and mouse cortical thick ascending limb (CTAL) as evidenced by the properties of the dose-dependent increase in the concentration of intracellular calcium ([Ca 2ϩ ] i ) as a function of peritubular [Ca 2ϩ ] e (11, 12). High [Ca 2ϩ ] e (5 mM) in the mouse CTAL decreases hormonedependent cAMP accumulation, an effect which has been ascribed to a direct inhibition of adenylyl cyclase (AC) activity (13).Experiments using quantitative reverse transcription-polymerase chain reaction (RT-PCR) have shown that the rat CTAL also expresses the Ca 2ϩ -inhibitable type 6 AC mRNA (14). All the G␣ s -coupled receptors studied so far in this segment activate a single pool of AC catalytic units (15,16), and in addition, electron microscopy studies describe a single cell type in this epithelium (17). These observations lead to the hypothesis that the functional expression of the type 6 AC mRNA accounts for the hormone-dependent cAMP synthesis in the rat CTAL.The aim of the present study was therefore to investigate the functional expression of the AC present in the rat CTAL and the consequences of the possible co-localization in a same cell of a Ca 2ϩ -inhibitable AC and of a Ca 2ϩ -sensing receptor on the regulation of cAMP synthesis and/or hydrolysis. In order to study the regulation of cAMP levels elicited by potentially similar or different mechanisms of action, we compared the effect of extracellular Ca 2ϩ to those of two agents also active in this segment. The first agent, angiotensin II, induces [Ca 2ϩ ] i increases in the rat CTAL (11,18). The pattern of the responses * This work was supported in part by Grant URA 1859 from the Centre National de la Recherche Scientifique and from the CEA (DBCM, SBCe). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. ; CT...

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Co-expression of a Ca2+-inhibitable Adenylyl Cyclase and of a Ca2+-sensing Receptor in the Cortical Thick Ascending Limb Cell of the Rat Kidney

Ferreira¹,

Héliès-Toussaint²,

Imbert–Teboul

et al. 1998

Journal of Biological Chemistry

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“…However, pertussis toxin, an inhibitor of Gi, does not prevent PGE2 antagonizing the effects of AVP in cultured rabbit cortical collecting duct cells or in isolated rat outer medullary collecting ducts (Noland et al, 1992;Aarab et al, 1993;Aarab et al, 1999). In IMCD and Fig.…”

Section: Journal Of Cell Science 116 (16)mentioning

confidence: 99%

The prostaglandin E2 analogue sulprostone antagonizes vasopressin-induced antidiuresis through activation of Rho

Tamma

Wiesner

Furkert

et al. 2003

Journal of Cell Science

103

107

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Dynamic and structural scalings of the complexation between pDNA and bPEI in semidilute and low‐salt solutions

et al. 2010

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Using a combination of static and dynamic laser light scattering, we investigated the complexation of a supercoiled plasmid DNA (pDNA, 10(4) bp) and a branched polyethyleneimine (bPEI, M(w) = 25 kD) in semidilute and low-salt aqueous solutions. Our results unearth some scaling laws for dynamic and structural properties of the resultant complexes (polyplexes) with different bPEI:pDNA (N:P) molar ratios. Namely, the average scattering intensity () and the average linewidth of the Rayleigh peak () are scaled to the scattering vector (q) as proportional, variant q(alpha(s) ) or proportional, variant q(alpha(D) ), where alpha(S) and alpha(D) are two N:P dependent scaling exponents. The N:P ratio strongly affects the complexation. When N:P < 2.0, the motions of the negatively charged and extended pDNA chains and the polyplexes are highly correlated so that they behave like a transient network with a fractal dimension. As the N:P ratio increases, nearly all of pDNA chains condensed and the overall charge of the polyplexes reverses to slightly positive, resulting in a turbid dispersion of large loose aggregates made of smaller, but more compact, polyplexes. Further increase of N:P finally disrupts large loose aggregates, leading to a homogeneous transparent dispersion of the polyplexes.

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PGE2-induced inhibition of AVP-dependent cAMP accumulation in the OMCD of the rat kidney is cumulative with respect to the effects of ?2-adrenergic and A1-adenosine agonists, insensitive to pertussis toxin and dependent on extracellular calcium

Abstract: Vasopressin (AVP) plays a key role in maximal urine concentration by stimulating NaCi reabsorption in the medullary thick ascending limbs of Henle (MAL) and by increasing water permeability in the medullary collecting tubules (MCT). These effects of AVP in MAL and MCT are mediated by cAMP.

Cited by 17 publications

References 26 publications

Co-expression of a Ca2+-inhibitable Adenylyl Cyclase and of a Ca2+-sensing Receptor in the Cortical Thick Ascending Limb Cell of the Rat Kidney

Co-expression of a Ca2+-inhibitable Adenylyl Cyclase and of a Ca2+-sensing Receptor in the Cortical Thick Ascending Limb Cell of the Rat Kidney

The prostaglandin E2 analogue sulprostone antagonizes vasopressin-induced antidiuresis through activation of Rho

Dynamic and structural scalings of the complexation between pDNA and bPEI in semidilute and low‐salt solutions

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