PGE2 promotes macrophage recruitment and neovascularization in murine wet-type AMD models

Zhan, Pengfei; Cui, Yuqing; Cao, Ye; Bao, Xianwen; Wu, Mei‐Li; Yang, Qian; Yang, Jing; Zheng, Haohan; Zou, Jian; Xie, Ting; Cai, Jiping; Yao, Yong; Wang, Xiaolu

doi:10.1186/s12964-022-00973-6

Cited by 7 publications

(5 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…M1 mainly secrete proinflammatory cytokines such as Tumor necrosis factor-α (TNF-α) and IL-1, IL-6 which are responsible for the recruitment of immune cells at the defect and initiation of the acute inflammatory response, leading to inflammation, tissue injury and fibrosis [ 51 ]. IL-10, related with M1 augmentation, plays a central role in tissue repair and neovascularization [ 13 , 52 ], increasing osteogenesis and decreasing osteoclastogenesis via activating exosomal IL-10 mRNA to cells, directly [ 13 , 39 , 53 ]. Thereby, M1 lay the foundation for subsequent bone tissue repair [ 17 ].…”

Section: Discussionmentioning

confidence: 99%

Doped Electrospinned Material-Guides High Efficiency Regional Bone Regeneration

et al. 2023

View full text Add to dashboard Cite

The main target of bone tissue engineering is to design biomaterials that support bone regeneration and vascularization. Nanostructured membranes of (MMA)1-co-(HEMA)1/(MA)3-co-(HEA)2 loaded with 5% wt of SiO2-nanoparticles (Si-M) were doped with zinc (Zn-Si-M) or doxycycline (Dox-Si-M). Critical bone defects were effectuated on six New Zealand-bred rabbit skulls and then they were covered with the membranes. After six weeks, a histological analysis (toluidine blue technique) was employed to determine bone cell population as osteoblasts, osteoclasts, osteocytes, M1 and M2 macrophages and vasculature. Membranes covering the bone defect determined a higher count of bone cells and blood vessels than in the sham group at the top regions of the defect. Pro-inflammatory M1 appeared in a higher number in the top regions than in the bottom regions, when Si-M and Dox-Si-M were used. Samples treated with Dox-Si-M showed a higher amount of anti-inflammatory and pro-regenerative M2 macrophages. The M1/M2 ratio obtained its lowest value in the absence of membranes. On the top regions, osteoblasts were more abundant when using Si-M and Zn-Si-M. Osteoclasts were equally distributed at the central and lateral regions. The sham group and samples treated with Zn-Si-M attained a higher number of osteocytes at the top regions. A preferential osteoconductive, osteoinductive and angiogenic clinical environment was created in the vicinity of the membrane placed on critical bone defects.

show abstract

Section: Discussionmentioning

confidence: 99%

Doped Electrospinned Material-Guides High Efficiency Regional Bone Regeneration

et al. 2023

View full text Add to dashboard Cite

show abstract

“…M2 macrophages are closely associated with VEGF production ( 132 , 140 ). The histone acetyltransferase p300/spliced X-box binding protein 1/homocysteine-inducible endoplasmic reticulum protein with ubiquitin-like domain 1 ( 115 ), CSF1/CSF-1R ( 116 ) and prostaglandin E2/E-prostanoid 1 receptor/protein kinase C axis ( 117 ) can all, when hypoxic circumstances exist, stimulate M2 macrophage polarization, hence increase choroidal vascular endothelial cell proliferation, migration, and tube formation. M2 macrophages express leukotriene B4 (LTB4) receptor 1 (BLT1) which is drawn to the LTB to generate VEGF-A in a BLT1-dependent manner, inducing choroidal neovascularization formation ( 141 ).…”

Section: Mechanisms Of Polarization In Drmentioning

confidence: 99%

“… Mechanisms of macrophage polarization in ocular diseases. The main DR-related mechanisms are circled in red ( 91 , 108 , 109 , 115 – 117 , 134 , 147 , 148 ). Created with .…”

Section: Mechanisms Of Polarization In Drmentioning

confidence: 99%

Macrophage/microglia polarization for the treatment of diabetic retinopathy

Yao,

Li,

Zhou

et al. 2023

Front. Endocrinol.

View full text Add to dashboard Cite

Macrophages/microglia are immune system defense and homeostatic cells that develop from bone marrow progenitor cells. According to the different phenotypes and immune responses of macrophages (Th1 and Th2), the two primary categories of polarized macrophages/microglia are those conventionally activated (M1) and alternatively activated (M2). Macrophage/microglial polarization is a key regulating factor in the development of inflammatory disorders, cancers, metabolic disturbances, and neural degeneration. Macrophage/microglial polarization is involved in inflammation, oxidative stress, pathological angiogenesis, and tissue healing processes in ocular diseases, particularly in diabetic retinopathy (DR). The functional phenotypes of macrophages/microglia affect disease progression and prognosis, and thus regulate the polarization or functional phenotype of microglia at different DR stages, which may offer new concepts for individualized therapy of DR. This review summarizes the involvement of macrophage/microglia polarization in physiological situations and in the pathological process of DR, and discusses the promising role of polarization in personalized treatment of DR.

show abstract

“…M2 polarization has been regarded as a key promoter in various angiogenetic disorders, including choroidal neovascularization (CNV) and retinal neovascularization (RNV) [ 4 , 5 ]. Increased PGE2 in wAMD participates in M2 polarization and IL-10 production via the EP1R/ PKC signaling pathway, which additionally promotes the proliferation and migration of human choroidal microvascular endothelial cells (HCECs) in vitro [ 6 ]. Furthermore, inhibiting the RhoA/ROCK signaling pathway facilitated Mφs to transform from the M2 to the M1 phenotype, reducing vascular leakage and abnormal proliferation in CNV [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Succinate-induced macrophage polarization and RBP4 secretion promote vascular sprouting in ocular neovascularization

Shen,

Lin,

et al. 2023

J Neuroinflammation

View full text Add to dashboard Cite

Pathological neovascularization is a pivotal biological process in wet age-related macular degeneration (AMD), retinopathy of prematurity (ROP) and proliferative diabetic retinopathy (PDR), in which macrophages (Mφs) play a key role. Tip cell specialization is critical in angiogenesis; however, its interconnection with the surrounding immune environment remains unclear. Succinate is an intermediate in the tricarboxylic acid (TCA) cycle and was significantly elevated in patients with wet AMD by metabolomics. Advanced experiments revealed that SUCNR1 expression in Mφ and M2 polarization was detected in abnormal vessels of choroidal neovascularization (CNV) and oxygen-induced retinopathy (OIR) models. Succinate-induced M2 polarization via SUCNR1, which facilitated vascular endothelial cell (EC) migration, invasion, and tubulation, thus promoting angiogenesis in pathological neovascularization. Furthermore, evidence indicated that succinate triggered the release of RBP4 from Mφs into the surroundings to regulate endothelial sprouting and pathological angiogenesis via VEGFR2, a marker of tip cell formation. In conclusion, our results suggest that succinate represents a novel class of vasculature-inducing factors that modulate Mφ polarization and the RBP4/VEGFR2 pathway to induce pathological angiogenic signaling through tip cell specialization. Graphical Abstract

show abstract

PGE2 promotes macrophage recruitment and neovascularization in murine wet-type AMD models

Cited by 7 publications

References 31 publications

Doped Electrospinned Material-Guides High Efficiency Regional Bone Regeneration

Doped Electrospinned Material-Guides High Efficiency Regional Bone Regeneration

Macrophage/microglia polarization for the treatment of diabetic retinopathy

Succinate-induced macrophage polarization and RBP4 secretion promote vascular sprouting in ocular neovascularization

Contact Info

Product

Resources

About