pH-Independent Recognition of Polyhydroxy Compounds by Niobium(V) Porphyrin Complex with Unique Sugar Selectivity

Doi, Takuya; Kachikawa, Norihide; Yasui, Takashi; Yuchi, Akio

doi:10.2116/analsci.33.1351

Cited by 1 publication

(1 citation statement)

References 25 publications

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“…As shown in Figure S7, the frequency shifts caused by fructose or mannose were similar to that by glucose. In line with the selectivity between the PBA group and various sugars (fructose > sorbose ≫ arabinose > galactose > mannose > glucose), more cells were detached from the PBAS film in the presence of fructose or mannose.…”

Section: Resultsmentioning

confidence: 67%

Real-Time Evaluation of Adhesion Processes and Glucose Response of Cancer Cells onto Phenylboronic Acid-Functionalized Films Monitored by Quartz Crystal Microbalance with Dissipation

Wang,

Sun,

et al. 2023

Anal. Chem.

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Understanding the interactions between cancer cells and smart substrates is of great benefit to physiology and pathology. Herein, we successfully fabricated two phenylboronic acid (PBA)-functionalized films with different surface topographies using a PBA homopolymer (PBAH) and self-assembled nanoparticles (PBAS) via a layer-bylayer assembly technique. We used a quartz crystal microbalance with dissipation (QCM-D) to monitor the entire cell adhesion process and figured out the adhesion kinetics of HepG2 cells on the two PBA-functionalized films. As seen from the QCM-D data, the HepG2 cells displayed distinctly different adhesion behaviors on the two PBAfunctionalized films (PBAS and PBAH films). The results showed that the PBAS film promoted cell adhesion and cell spreading owing to its specific physicochemical properties. Likewise, the slope changes in the D-f plots clearly revealed the evolution of the cell adhesion process, which could be classified into three stages during cell adhesion on the PBA-functionalized films. In addition, compared with the PBAH film, the PBAS film could also control cell detachment behavior in the presence of glucose based on the molecular recognition between the PBA group and the cell membrane. Such a glucose-responsive PBAS film is promising for biological applications, including cell-based diagnostics and tissue engineering. In addition, the QCM-D proved to be a useful tool for in situ and real-time monitoring and analysis of interactions between cells and surfaces of supporting substrates.

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Section: Resultsmentioning

confidence: 67%