pH-induced conformational change of natural cyclic lipopeptide surfactin and the effect on protease activity

Taira, Toshiaki; Yanagisawa, Satohiro; Nagano, Tadayoshi; Tsuji, T; Endo, Akira; Imura, Tomohiro

doi:10.1016/j.colsurfb.2017.05.017

Cited by 20 publications

(8 citation statements)

References 29 publications

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“…In our opinion, increasing the pH above 6.0 probably increased the negative charge of the polar head of surfactin, where the pKa values of Asp and Glu are around 4.3 and 4.5, thus enhancing its solubility in water. The maximum surface activity of surfactin was observed at pHs 6.0-8.0, as the pKa value of non-dissociated surfactin is about 6.0 [49]. Under highly acidic conditions (pH 2.0 and 4.0) surfactin precipitates, thus leading to much lower surface activity.…”

Section: Effect Of Ph Temperature and Salinity On Biosurfactant Activitymentioning

confidence: 97%

Sustainable Surfactin Production by Bacillus subtilis Using Crude Glycerol from Different Wastes

et al. 2021

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Most biosurfactants are obtained using costly culture media and purification processes, which limits their wider industrial use. Sustainability of their production processes can be achieved, in part, by using cheap substrates found among agricultural and food wastes or byproducts. In the present study, crude glycerol, a raw material obtained from several industrial processes, was evaluated as a potential low-cost carbon source to reduce the costs of surfactin production by Bacillus subtilis #309. The culture medium containing soap-derived waste glycerol led to the best surfactin production, reaching about 2.8 g/L. To the best of our knowledge, this is the first report describing surfactin production by B. subtilis using stearin and soap wastes as carbon sources. A complete chemical characterization of surfactin analogs produced from the different waste glycerol samples was performed by liquid chromatography–mass spectrometry (LC-MS) and Fourier transform infrared spectroscopy (FTIR). Furthermore, the surfactin produced in the study exhibited good stability in a wide range of pH, salinity and temperatures, suggesting its potential for several applications in biotechnology.

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Section: Effect Of Ph Temperature and Salinity On Biosurfactant Activitymentioning

confidence: 97%

Sustainable Surfactin Production by Bacillus subtilis Using Crude Glycerol from Different Wastes

et al. 2021

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“…On the contrary, the linearization of the peptide cycle (CMC linear surfactin C14 = 374 µM in Tris pH 8.5) (Dufour et al, 2005) and the presence of a Leu4 instead of the Val4 as in pumilacidin (de Araujo et al, 2019) increase it. Different self-assembled nanostructures like sphere-like micelles, wormlike micelles and unilamellar bilayers coexist with larger aggregates in aqueous solution depending on the surfactin concentration, pH, temperature, ionic strength and metal ions (Zou et al, 2010;Taira et al, 2017;Jahan et al, 2020). These parameters can induce conformational changes in the secondary structure of the cyclic peptide moiety and thereby affect the shape and the packing parameter of surfactin (Jahan et al, 2020).…”

Section: Surfactin Structure and Its Influence On Physico-chemical Prmentioning

confidence: 99%

The Surfactin-Like Lipopeptides From Bacillus spp.: Natural Biodiversity and Synthetic Biology for a Broader Application Range

Théatre

Cano-Prieto

Bartolini

et al. 2021

Front. Bioeng. Biotechnol.

118

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Surfactin is a lipoheptapeptide produced by several Bacillus species and identified for the first time in 1969. At first, the biosynthesis of this remarkable biosurfactant was described in this review. The peptide moiety of the surfactin is synthesized using huge multienzymatic proteins called NonRibosomal Peptide Synthetases. This mechanism is responsible for the peptide biodiversity of the members of the surfactin family. In addition, on the fatty acid side, fifteen different isoforms (from C12 to C17) can be incorporated so increasing the number of the surfactin-like biomolecules. The review also highlights the last development in metabolic modeling and engineering and in synthetic biology to direct surfactin biosynthesis but also to generate novel derivatives. This large set of different biomolecules leads to a broad spectrum of physico-chemical properties and biological activities. The last parts of the review summarized the numerous studies related to the production processes optimization as well as the approaches developed to increase the surfactin productivity of Bacillus cells taking into account the different steps of its biosynthesis from gene transcription to surfactin degradation in the culture medium.

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“…Hence, components of common mouthwash agents have adverse side effects associated with them. Alternatively, biosurfactants (rhamnolipids and surfactin) have low toxicity and are eco-friendly [ 62 , 63 ], and may, therefore, provide a superior option for improved oral care. Rhamnolipids, specifically, may serve as an optimal choice as a preventive medicine for periodontal disease as it demonstrated strong anti-bacterial and anti-biofilm activity against A. actinomycetemcomitans Y4.…”

Section: Discussionmentioning

confidence: 99%

Rhamnolipids and surfactin inhibit the growth or formation of oral bacterial biofilm

et al. 2020

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Background Bacteria survive in various environments by forming biofilms. Bacterial biofilms often cause significant problems to medical instruments and industrial processes. Techniques to inhibit biofilm formation are essential and have wide applications. In this study, we evaluated the ability of two types of biosurfactants (rhamnolipids and surfactin) to inhibit growth and biofilm formation ability of oral pathogenic bacteria such as Aggregatibacter actinomycetemcomitans, Streptococcus mutans, and Streptococcus sanguinis. Results Rhamnolipids inhibited the growth and biofilm formation ability of all examined oral bacteria. Surfactin showed effective inhibition against S. sanguinis ATCC10556, but lower effects toward A. actinomycetemcomitans Y4 and S. mutans UA159. To corroborate these results, biofilms were observed by scanning electron microscopy (SEM) and confocal microscopy. The observations were largely in concordance with the biofilm assay results. We also attempted to determine the step in the biofilm formation process that was inhibited by biosurfactants. The results clearly demonstrated that rhamnolipids inhibit biofilm formation after the initiation process, however, they do not affect attachment or maturation. Conclusions Rhamnolipids inhibit oral bacterial growth and biofilm formation by A. actinomycetemcomitans Y4, and may serve as novel oral drug against localized invasive periodontitis.

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pH-induced conformational change of natural cyclic lipopeptide surfactin and the effect on protease activity

Cited by 20 publications

References 29 publications

Sustainable Surfactin Production by Bacillus subtilis Using Crude Glycerol from Different Wastes

Sustainable Surfactin Production by Bacillus subtilis Using Crude Glycerol from Different Wastes

The Surfactin-Like Lipopeptides From Bacillus spp.: Natural Biodiversity and Synthetic Biology for a Broader Application Range

Rhamnolipids and surfactin inhibit the growth or formation of oral bacterial biofilm

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