pH-induced morphological changes of proteinaceous viral shells

Roshal, Daria S.; Konevtsova, O.V.; Božič, Anže; Podgornik, Rudolf; Rochal, S.B.

doi:10.1038/s41598-019-41799-6

Cited by 21 publications

(9 citation statements)

References 35 publications

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“…Cryo-EM of the CIRV particle showed that they have internal shells with the separates section. The outer surfaces of the shells have a negative charge, and the inner surfaces have a positive charge due to pH-induced morphological changes of proteinaceous viral shells 12,13 . It was demonstrated that p19 and other proteins staying in the virus based on the changes in the charges 14 .…”

mentioning

confidence: 99%

Impediometric Electrochemical Sensor Based on The Inspiration of Carnation Italian Ringspot Virus Structure to Detect an Attommolar of miR

Ghazizadeh

Moosavifard

Daneshmand

et al. 2020

Sci Rep

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electrochemical sensors are the tools to detect the accurate and sensitive miRs. there is the challenge to increase the power and sensitivity of the surface for the electrochemical sensor. We design a viruslike hallow structure of cuco 2 o 4 that it holds the large amounts of p19 protein by mimicking of inherent virus (Carnation italian ringspot virus) to detect 21mir with the limit of detection (LOD = 1aM). The electrochemical measurements are performed between the potentials at −0.3 V and +0.3 V with 1 mM [fe(cn) 6 ] −3/−4. After dropping the cuco 2 o 4 on the Scpe (screen carbon printed electrode), the sensor is turned on due to the high electrochemical properties. Then, p19 proteins move into the hallow structure and inhibit the exchange of electrochemical reactions between the shells and the sensor is turned off. Then, adding the duplexes of RNA/miRs cause to increase the electrochemical property of p19 due to the change of p19 conformation and the system is turned on, again. So, for the first time, a virus-like hallow structure has been used to detect the 21miR in the human serum, MCF-7, Hella cells, with high sensitivity, specificity, and reproducibility in few minutes.

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confidence: 99%

Impediometric Electrochemical Sensor Based on The Inspiration of Carnation Italian Ringspot Virus Structure to Detect an Attommolar of miR

Ghazizadeh

Moosavifard

Daneshmand

et al. 2020

Sci Rep

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“…They were considered to be responsible for the pH-dependent changes in the morphology and assembly/disassembly of the viral capsid of noroviruses. Although viral capsids have some elastic behavior, capsid disintegration may take place if large highly inhomogeneous deformations in the viral capsid (overstretch) occur due to the repulsion between the identical charges (negative charges at high pH) (da Silva et al, 2011;Roshal et al, 2019). At neutral and high pH, most viral particles have a net negative charge because they have an isoelectric point below 7.…”

Section: Alkaline Solutionsmentioning

confidence: 99%

Risks and new challenges in the food chain: Viral contamination and decontamination from a global perspective, guidelines, and cleaning

Ezzatpanah

Gómez‐López

Koutchma

et al. 2022

Comp Rev Food Sci Food Safe

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Even during the continuing world pandemic of severe acute respiratory syndrome coronavirus 2 (SARS CoV‐2), consumers remain exposed to the risk of getting infected by existing, emerging, or re‐emerging foodborne and waterborne viruses. SARS‐CoV‐2 is different in that it is transmitted directly via the airborne route (droplets and aerosols) or indirect contact (surfaces contaminated with SARS‐CoV‐2). International food and health organizations and national regulatory bodies have provided guidance to protect individuals active in food premises from potential occupational exposure to SARS‐CoV‐2, and have recommended chemicals effective in controlling the virus. Additionally, to exclude transmission of foodborne and waterborne viruses, hygiene practices to remove viral contaminants from surfaces are applied in different stages of the food chain (e.g., food plants, food distribution, storage, retail sector, etc.), while new and enhanced measures effective in the control of all types of viruses are under development. This comprehensive review aims to analyze and compare efficacies of existing cleaning practices currently used in the food industry to remove pathogenic viruses from air, nonfood, and food contact surfaces, as well as from food surfaces. In addition, the classification, modes of transmission, and survival of food and waterborne viruses, as well as SARS‐CoV‐2 will be presented. The international guidelines and national regulations are summarized in terms of virucidal chemical agents and their applications.

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“…Droplets can also vary in the value of their pH [56,[187][188][189], which can change with RH and with that affect the viability of aerosolized viruses in the droplets. Already from a purely electrostatic perspective, a change in pH modifies the charge on the ionizable amino acids [190,191], which can lead to conformational changes in viral proteins [141,192], eventually destabilizing the virus [193][194][195]. Yang et al [141] furthermore found that the response of enveloped viruses to changes in RH depends on whether the process of fusion requires low pH or not.…”

Section: Droplet Phmentioning

confidence: 99%

Relative humidity in droplet and airborne transmission of disease

Božič,

Kanduč

2020

Preprint

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A large number of infectious diseases is transmitted by respiratory droplets. How long these droplets persist in the air, how far they can travel, and how long the pathogens they might carry survive are all decisive factors for the spread of dropletborne diseases. The subject is extremely multifaceted and its aspects range across different disciplines, yet most of them have only seldom been considered in the physics community. In this review, we discuss the physical principles that govern the fate of respiratory droplets and any viruses trapped inside them, with a focus on the role of relative humidity. Importantly, low relative humidity-as encountered, for instance, indoors during winter and inside aircraft-facilitates evaporation and keeps even initially large droplets suspended in air as aerosol for extended periods of time. What is more, relative humidity affects the stability of viruses in aerosol through several physical mechanisms such as efflorescence and inactivation at the air-water interface, whose role in virus inactivation nonetheless remains poorly understood. Elucidating the role of relative humidity in the droplet spread of disease would permit us to design preventive measures that could aid in reducing the chance of transmission, particularly in indoor environment. Keywords relative humidity • droplets • airborne transmission • viruses 1 IntroductionOne of the prevalent ways in which numerous viruses, bacteria, and fungi spread among plants, animals, and humans is by droplets of various sizes [1][2][3]. Humans produce respiratory droplets during talking, coughing, sneezing, and other similar activ-

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pH-induced morphological changes of proteinaceous viral shells

Cited by 21 publications

References 35 publications

Impediometric Electrochemical Sensor Based on The Inspiration of Carnation Italian Ringspot Virus Structure to Detect an Attommolar of miR

Impediometric Electrochemical Sensor Based on The Inspiration of Carnation Italian Ringspot Virus Structure to Detect an Attommolar of miR

Risks and new challenges in the food chain: Viral contamination and decontamination from a global perspective, guidelines, and cleaning

Relative humidity in droplet and airborne transmission of disease

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