pH measurement of tubular vacuoles of an arbuscular mycorrhizal fungus, Gigaspora margarita

Funamoto, Rintaro; Saito, Katsuichi; Oyaizu, Hiroshi; Aono, Toshihiro; Saitô, Masanori

doi:10.1007/s00572-014-0588-1

Cited by 5 publications

(4 citation statements)

References 33 publications

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“…The histochemical staining of alkaline phosphatase is considered to be a marker of polyphosphate metabolic activity localized in fungal tissues within the roots, and usually coincides with plant growth stimulation. It is also known that the intensity of alkaline phosphatase decreases with the age of colonization or when soluble phosphate is added to the substrate, and that this metabolic activity is associated with mycorrhizal functionality and ability to transfer phosphate to the plant (Tisserant et al, 1993;Vivas et al, 2003;Aono et al, 2004;Funamoto et al, 2007;Zhang et al, 2023). Our data are partially consistent with these previous observations when comparing both fungal phenotypes under low Pi.…”

Section: Discussionsupporting

confidence: 91%

Short-term artificial adaptation of Rhizoglomus irregulare to high phosphate levels and its implications for fungal-plant interactions: phenotypic and transcriptomic insights

Lucic-Mercy,

Mercy,

Jeschke

et al. 2024

Front. Plant Sci.

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Arbuscular mycorrhizal fungi (AMF) play a crucial role in enhancing plant growth, but their use in agriculture is limited due to several constraints. Elevated soil phosphate levels resulting from fertilization practices strongly inhibit fungal development and reduce mycorrhizal growth response. Here, we investigated the possibility of adapting Rhizoglomus irregulare to high phosphate (Pi) levels to improve its tolerance. A fungal inoculum was produced through multiple generations in the presence of elevated Pi and used to inoculate melon plants grown under low and high phosphate conditions. Our results revealed distinct phenotypic and transcriptomic profiles between the adapted and non-adapted Rhizoglomus irregulare. The Pi adapted phenotype led to enhanced root colonization under high Pi conditions, increased vesicle abundance, and higher plant biomass at both phosphate levels. Additionally, the adaptation status influenced the expression of several genes involved in Pi uptake, Pi signaling, and mitochondrial respiration in both symbiotic partners. While the underlying mechanisms of the adaptation process require further investigation, our study raises intriguing questions. Do naturally occurring phosphate-tolerant AMF already exist? How might the production and use of artificially produced inocula bias our understanding? Our findings shed light on the adaptive capacities of Glomeromycota and challenge previous models suggesting that plants control mycorrhizal fungal growth. Moreover, our work pave the way for the development of innovative biotechnological tools to enhance the efficacy of mycorrhizal inoculum products under practical conditions with high phosphate fertilization.

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Section: Discussionsupporting

confidence: 91%

Short-term artificial adaptation of Rhizoglomus irregulare to high phosphate levels and its implications for fungal-plant interactions: phenotypic and transcriptomic insights

Lucic-Mercy,

Mercy,

Jeschke

et al. 2024

Front. Plant Sci.

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“…Therefore, it is highly possible that arbuscule collapse contributes to cytoplasmic streaming and plays an important role in resource utilization in the mycelium of AM fungi. Future studies exploring the counter translocations of lipid droplets and tubular vacuoles designed for polyphosphate storage (Kuga et al 2008, Funamoto et al 2014 within both intraradical and extraradical mycelium should evaluate how such resource exchange occurs throughout the mycelium.…”

Section: Discussionmentioning

confidence: 99%

Lipid Droplets of Arbuscular Mycorrhizal Fungi Emerge in Concert with Arbuscule Collapse

Kobae

Gutjahr²,

Paszkowski

et al. 2014

Plant and Cell Physiology

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Plants share photosynthetically fixed carbon with arbuscular mycorrhizal (AM) fungi to maintain their growth and nutrition. AM fungi are oleogenic fungi that contain numerous lipid droplets in their syncytial mycelia during most of their life cycle. These lipid droplets are probably used for supporting growth of extraradical mycelia and propagation; however, when and where the lipid droplets are produced remains unclear. To address these issues, we investigated the correlation between intracellular colonization stages and the appearance of fungal lipid droplets in roots by a combination of vital staining of fungal structures, selective staining of lipids and live imaging. We discovered that a surge of lipid droplets coincided with the collapse of arbuscular branches, indicating that arbuscule collapse and the emergence of lipid droplets may be associated processes. This phenomenon was observed in the model AM fungus Rhizophagus irregularis and the ancestral member of AM fungi Paraglomus occultum. Because the collapsing arbuscules were metabolically inactive, the emerged lipid droplets are probably derived from preformed lipids but not de novo synthesized. Our observations highlight a novel mode of lipid release by AM fungi.

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“…We demonstrated for the first time that VTC4 catalyzes the reverse reaction (the regeneration of ATP from polyP) by using AM fungal VTC4. Given that the nucleotide substrate ATP is supplied in the cytosol in the forward reaction in S. cerevisiae (Müller et al, 2003;Hothorn et al, 2009a), it is highly likely that the catalytic domain of RiVTC4 also faces the cytosol, which is indirectly supported by our result of an optimum pH between 7 and 8, which is the cytosolic pH range of the fungi (Jolicoeur et al, 1998;Funamoto et al, 2014). Therefore, we consider that the direction of the reactions is dependent on the ATP:ADP concentration ratios in the cytosol.…”

Section: Discussionmentioning

confidence: 74%

Polyphosphate polymerizing and depolymerizing activity of VTC4 protein in an arbuscular mycorrhizal fungus

Cúc

Ezawa

Saito

2022

Soil Science and Plant Nutrition

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pH measurement of tubular vacuoles of an arbuscular mycorrhizal fungus, Gigaspora margarita

Abstract: Arbuscular mycorrhizal fungi play an important role in phosphate supply to the host plants.

Cited by 5 publications

References 33 publications

Short-term artificial adaptation of Rhizoglomus irregulare to high phosphate levels and its implications for fungal-plant interactions: phenotypic and transcriptomic insights

Short-term artificial adaptation of Rhizoglomus irregulare to high phosphate levels and its implications for fungal-plant interactions: phenotypic and transcriptomic insights

Lipid Droplets of Arbuscular Mycorrhizal Fungi Emerge in Concert with Arbuscule Collapse

Polyphosphate polymerizing and depolymerizing activity of VTC4 protein in an arbuscular mycorrhizal fungus

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