pH modulation ameliorates the red blood cell storage lesion in a murine model of transfusion

Chang, Alex L.; Kim, Young; Seitz, Amy E.; Schuster, Rebecca; Pritts, Timothy A.

doi:10.1016/j.jss.2016.12.025

Cited by 15 publications

(10 citation statements)

References 35 publications

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“…Treatment for MAlk are discussed in section 3.3.2. Finally, a study in mice suggests that raising the pH of stored blood enhances red blood survival after transfusion [368] . The pH-dependence of wound healing suggests that therapeutic maintenance of the pH of the wound or graft could aid healing [366] .…”

Section: Other Applications By Clinical Specialtymentioning

confidence: 99%

The therapeutic importance of acid-base balance

Quade

Parker

Occhipinti

2021

Biochemical Pharmacology

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Baking soda and vinegar have been used as home remedies for generations and today we are only a mouse-click away from claims that baking soda, lemon juice, and apple cider vinegar are miracles cures for everything from cancer to COVID-19. Despite these specious claims, the therapeutic value of controlling acid-base balance is indisputable and is the basis of Food and Drug Administration-approved treatments for constipation, epilepsy, metabolic acidosis, and peptic ulcers. In this narrative review, we present evidence in support of the current and potential therapeutic value of countering local and systemic acid-base imbalances, several of which do in fact involve the administration of baking soda (sodium bicarbonate). Furthermore, we discuss the side effects of pharmaceuticals on acid-base balance as well as the influence of acid-base status on pharmacokinetic properties of drugs. Our review considers all major organs systems as well as information relevant to several clinical specialties such as anesthesiology, infectious disease, oncology, dentistry, and surgery.

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Section: Other Applications By Clinical Specialtymentioning

confidence: 99%

The therapeutic importance of acid-base balance

Quade

Parker

Occhipinti

2021

Biochemical Pharmacology

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“…Stored RBCs undergo considerable shape change from a normal smooth discoid shape (discocyte) to a spheroid shape with speculae (echinocyte). While some of these changes are reversible, others are irreversible, thus making the RBC dysfunctional [ 15 , 16 ]. In our studies, we found that the percent of normal RBCs were higher on Day 56 in RBC stored in VitC ( Figure 8 ).…”

Section: Resultsmentioning

confidence: 99%

“…Hemolysis was intensified in erythrocytes obtained from glucose-6-phosphate dehydrogenase (G6PD) deficient patients. Of importance in this work, erythrocytes were salvaged in Zhang’s work and in reports by others [ 14 ] by the addition of antioxidants, by alterations of pH [ 15 ] or by addition of alkaline CPD, which preserves erythrocyte 2,3-DPG. [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Attenuation of Red Blood Cell Storage Lesions with Vitamin C

et al. 2017

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Stored red blood cells (RBCs) undergo oxidative stress that induces deleterious metabolic, structural, biochemical, and molecular changes collectively referred to as “storage lesions”. We hypothesized that vitamin C (VitC, reduced or oxidized) would reduce red cell storage lesions, thus prolonging their storage duration. Whole-blood-derived, leuko-reduced, SAGM (saline-adenine-glucose-mannitol)-preserved RBC concentrates were equally divided into four pediatric storage bags and the following additions made: (1) saline (saline); (2) 0.3 mmol/L reduced VitC (Lo VitC); (3) 3 mmol/L reduced VitC (Hi VitC); or (4) 0.3 mmol/L oxidized VitC (dehydroascorbic acid, DHA) as final concentrations. Biochemical and rheological parameters were serially assessed at baseline (prior to supplementation) and Days 7, 21, 42, and 56 for RBC VitC concentration, pH, osmotic fragility by mechanical fragility index, and percent hemolysis, LDH release, glutathione depletion, RBC membrane integrity by scanning electron microscopy, and Western blot for β-spectrin. VitC exposure (reduced and oxidized) significantly increased RBC antioxidant status with varying dynamics and produced trends in reduction in osmotic fragility and increases in membrane integrity. Conclusion: VitC partially protects RBC from oxidative changes during storage. Combining VitC with other antioxidants has the potential to improve long-term storage of RBC.

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“…Literature suggests that alkalinization would be protective. Chang et al 30 found that, compared to standard AS‐3 with pH 5.8, acidification (pH 4.5) shortened circulating half‐life, while alkalinization (pH 8.5) increased circulating half‐life and decreased hemolysis. Alkalinization likely contributes to the prolonged blood storage capacity of AS‐7 vs AS‐1 31 .…”

Section: Discussionmentioning

confidence: 99%

Mixing commonly used crystalloid solutions with red blood cells in five common additives does not negatively impact hemolysis, aggregometry, or deformability

Yarnoff

Dodd‐o

2020

Transfusion

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Background: Literature is beginning to challenge the belief that it is unsafe to coinfuse red blood cells (RBCs) with solutions other than isotonic saline. We recently showed that additive-free RBCs tolerated coincubation with Plasma-Lyte or catecholamines dissolved in normal saline (NS), though 5% dextrose in water (D5W) promoted hemolysis. Herein, we evaluate the effect of coincubating crystalloids on additive-preserved RBC hemolysis, aggregation, and membrane deformability. Study Design and Methods: RBCs were coincubated 5 minutes with plasma, NS, Plasma-Lyte, lactated Ringer's (LR) or D5W (1 mL PRBC +131.3 μL solution). Samples were then assessed for hemolysis (free hemoglobin), aggregation (critical shear stress [mPa]), and membrane deformability (elongation index [EI]). Significance (P ≤ .05) by t test or ANOVA with post-hoc Tukey-Kramer test. Results: Additive-prepared RBCs coincubated with crystalloid instead of plasma demonstrated: (a) no increase in hemolysis as indicated by plasma free hemoglobin levels that is likely to be clinically relevant; (b) no increase, but in some cases a decrease, in aggregation as indicated by critical shear stress; and (c) in some combinations, a deterioration in deformability. When present, the deformability decrease was likely clinically insignificant in degree, and always returned to normal when the crystalloid was subsequently diluted out with plasma. Conclusion: Our data suggest that additive-prepared RBCs coincubated for 5 minutes with any of four common crystalloids demonstrate no clinically relevant increased lysis, increased aggregation, or decreased deformability.

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pH modulation ameliorates the red blood cell storage lesion in a murine model of transfusion

Cited by 15 publications

References 35 publications

The therapeutic importance of acid-base balance

The therapeutic importance of acid-base balance

Attenuation of Red Blood Cell Storage Lesions with Vitamin C

Mixing commonly used crystalloid solutions with red blood cells in five common additives does not negatively impact hemolysis, aggregometry, or deformability

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