pH responsive biohybrid BSA-poly(DPA) nanoparticles for interlysosomal drug delivery

Papadimitriou, Lila; Theodorou, Alexis; Papageorgiou, Maria; Voutyritsa, Errika; Papagiannaki, Argyri; Velonia, Kelly; Ranella, Anthi

doi:10.1016/j.jddst.2022.103591

Cited by 2 publications

(1 citation statement)

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“…Interestingly, by performing single LP tracking, we found that LP located in the peripheral area (ROI 1, red circle) showed more dynamic movement, while the LP adjacent to the nucleus ((ROI 2, orange circle) showed less movement (Figure 8c, Supplementary Movie 3, Supporting Information), consistent with the previous result. [ 52,53 ] Moreover, as illustrated in Figure 8a, the white arrow indicates complete colocalization of TF (h), DiD, and lysosomes over time, indicating the good combination of these two labels in LPs. However, there was also evidence that TF (h), DiD, and lysosome signals did not colocalize, or only two colocalized (Figure 8a, circles with different colors).…”

Section: Resultsmentioning

confidence: 95%

Precision Localization of Lipid‐Based Nanoparticles by Dual‐Fluorescent Labeling for Accurate and High‐Resolution Imaging in Living Cells

et al. 2023

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In nanomedicine, lipid‐based nanoparticles (NPs) such as liposomes (LPs) have established an important position. Precise delineation of NP interaction with cells and detailed characterization of activity are becoming essential, which mainly rely on labeling with lipophilic fluorescent molecules and assuming stable association with NPs. However, because of label separation from NPs in (biological) media, or when processed by cells, fluorescence‐based detection of an NP incorporating a single label may not necessarily indicate the actual presence of an NP but may be from the dissociated label, rendering results unreliable. Herein, flow cytometry and confocal microscopy are employed to demonstrate that to verify the localization of LPs in a cell with perfect accuracy, dual‐labeling, and contemporaneous detection of both fluorescent signals in one pixel are required. This is combined with size exclusion chromatography (SEC) and mass spectrometry measurements to indicate factors involved in label dissociation, which helps to understand the possible conditions of dissociated label and NP. It is shown that determining label colocalization with, and label dissociation from, dual‐labeled NPs are needed to provide accurate spatiotemporal insight into targeting destination (colocalized signals) and disintegration (separated signals) of NPs during intracellular processing and in studying payload delivery with precision in nanomedicine.

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Section: Resultsmentioning

confidence: 95%