pH-Responsive nanoplatform synergistic gas/photothermal therapy to eliminate biofilms in poly(<scp>l</scp>-lactic acid) scaffolds

Qian, Guowen; Mao, Yuqian; Zhao, Huihui; Zhang, Lemin; Xiong, Long; Long, Zhisheng

doi:10.1039/d3tb02600k

Cited by 10 publications

(6 citation statements)

References 56 publications

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“…They can regulate the biological functions of stem cells by combining with static magnetic field and can serve as a carrier for gene and drug transfection into cells, which has great potential for application in the field of bone tissue engineering. [31][32][33][34] However, as a kind of nanoparticles, MGO is not easily aggregated in the area of bone defects, so other scaffold materials or static magnetic fields need to be combined to repair large-scale bone defects. 7,35,36 Therefore, whether MGO has potential in BTE for craniomaxillofacial bone repair is worth further exploration.…”

Section: Discussionmentioning

confidence: 99%

Magnetic Graphene Oxide Nanocomposites Boosts Craniomaxillofacial Bone Regeneration by Modulating circAars/miR-128-3p/SMAD5 Signaling Axis

He,

Zhang,

Huang

et al. 2024

IJN

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Background The ability of nanomaterials to induce osteogenic differentiation is limited, which seriously imped the repair of craniomaxillofacial bone defect. Magnetic graphene oxide (MGO) nanocomposites with the excellent physicochemical properties have great potential in bone tissue engineering. In this study, we aim to explore the craniomaxillofacial bone defect repairment effect of MGO nanocomposites and its underlying mechanism. Methods The biocompatibility of MGO nanocomposites was verified by CCK8, live/dead staining and cytoskeleton staining. The function of MGO nanocomposites induced osteogenic differentiation of BMSCs was investigated by ALP activity detection, mineralized nodules staining, detection of osteogenic genes and proteins, and immune-histochemical staining. BMSCs with or without MGO osteogenic differentiation induction were collected and subjected to high-throughput circular ribonucleic acids (circRNAs) sequencing, and then crucial circRNA circAars was screened and identified. Bioinformatics analysis, Dual-luciferase reporter assay, RNA binding protein immunoprecipitation (RIP), fluorescence in situ hybridization (FISH) and osteogenic-related examinations were used to further explore the ability of circAars to participate in MGO nanocomposites regulation of osteogenic differentiation of BMSCs and its potential mechanism. Furthermore, critical-sized calvarial defects were constructed and were performed to verify the osteogenic differentiation induction effects and its potential mechanism induced by MGO nanocomposites. Results We verify the good biocompatibility and osteogenic differentiation improvement effects of BMSCs mediated by MGO nanocomposites. Furthermore, a new circRNA-circAars, we find and identify, is obviously upregulated in BMSCs mediated by MGO nanocomposites. Silencing circAars could significantly decrease the osteogenic ability of MGO nanocomposites. The underlying mechanism involved circAars sponging miR-128-3p to regulate the expression of SMAD5, which played an important role in the repair craniomaxillofacial bone defects mediated by MGO nanocomposites. Conclusion We found that MGO nanocomposites regulated osteogenic differentiation of BMSCs via the circAars/miR-128-3p/SMAD5 pathway, which provided a feasible and effective strategy for the treatment of craniomaxillofacial bone defects.

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Section: Discussionmentioning

confidence: 99%

Magnetic Graphene Oxide Nanocomposites Boosts Craniomaxillofacial Bone Regeneration by Modulating circAars/miR-128-3p/SMAD5 Signaling Axis

He,

Zhang,

Huang

et al. 2024

IJN

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“…Many studies have demonstrated that Zn and Si ions promote osteogenesis [30,31]. Shie et al [32] studied the effect of various concentrations of Si (0, 2, 4, and 6 mM) on osteoblast-like cell proliferation and apoptosis.…”

Section: Cell Behaviors On the Composite Scaffoldsmentioning

confidence: 99%

Synergistic effects of calcium silicate/zinc silicate dual compounds and in-situ interconnected pores on promoting bone regeneration of composite scaffolds

Qian,

Wu,

Wang

et al. 2024

Biomed. Mater.

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Rapid bone regeneration in implants is important for successful transplantation. In this regard, we report the development of calcium silicate/zinc silicate (CS/ZS) dual-compound-incorporated calcium phosphate cement (CPC) scaffolds with a three-dimensional poly (lactic-co-glycolic acid) network that synergistically promote bone regeneration. In vitro results demonstrated that the incorporation of CS/ZS dual compounds into the CPC significantly promoted the osteogenic differentiation of stem cells compared to the addition of CS or ZS alone. Moreover, the bone-regeneration efficacy of the composite scaffolds was validated by filling in femur condyle defects in rabbits, which showed that the scaffolds with CS and ZS possessed a great bone repair effect, as evidenced by more new bone formation and a faster scaffold biodegradation compared to the scaffold with CS alone.

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“…In this study, we first prepared a porous surface on PEEK by sulfonation and then grafted GO onto the sulfonated surface to explore how the modified surface influences the growth of bacteria [Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus bacteria; these two strains are the main pathogenic microbes responsible for implant-related infections ( Qian et al, 2024b )] and the osteogenic properties of cells [MC3T3-E1, a pre-osteoblast cell lineage; its ability to differentiate to osteoblasts is considered a precursor for the successful osteointegration of an implant ( Bhaskar et al, 2023 )]. In addition, the mechanical properties of the untreated/treated PEEK were also compared.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A self-assembling graphene oxide coating for enhanced bactericidal and osteogenic properties of poly-ether-ether-ketone

Huang,

Gu,

Yuan

et al. 2024

Front. Bioeng. Biotechnol.

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Poly-ether-ether-ketone (PEEK) is a biomedical plastic that can be used for orthopedic implants, but it offers poor antibacterial properties and bioactivity. In this study, PEEK was sulfonated with the obtained porous structure adsorbing graphene oxide (GO). The surface microstructures and properties of the original PEEK, sulfonated PEEK (SPEEK), and GO-grafted PEEK (GO-SPEEK) were characterized. The results revealed that the GO-SPEEK surface is a 3D porous structure exhibiting superior hydrophilicity to the original PEEK. Although SPEEK was shown to possess antimicrobial properties against both Escherichia coli and Staphylococcus aureus, the bactericidal effect was even more significant for GO-SPEEK, at about 86% and 94%, respectively. In addition, the in vitro simulated-body-fluid immersion and cell experiments indicated that GO-SPEEK had much better hydroxyapatite (HA)-precipitation induction capacity and cell–material interactions (e.g., cell adhesion, proliferation, osteodifferentiation, and extracellular matrix mineralization. The tensile test revealed that the mechanical properties of PEEK were maintained after surface modification, as GO-SPEEK has comparable values of elastic modulus and tensile strength to PEEK. Our investigation sought a method to simultaneously endow PEEK with both good antimicrobial properties and bioactivity as well as mechanical properties, providing a theoretical basis for developing high-performance orthopedic implants in the clinic.

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pH-Responsive nanoplatform synergistic gas/photothermal therapy to eliminate biofilms in poly(l-lactic acid) scaffolds

Abstract: The constructed ZnS–Ti3C2/PLLA scaffolds were able to release H2S gas to disrupt biofilm and further remove bacteria through photothermal. In addition, the Zn2+ released from the composite scaffolds could promote osteogenic differentiation of BMSCs.

Cited by 10 publications

References 56 publications

Magnetic Graphene Oxide Nanocomposites Boosts Craniomaxillofacial Bone Regeneration by Modulating circAars/miR-128-3p/SMAD5 Signaling Axis

Magnetic Graphene Oxide Nanocomposites Boosts Craniomaxillofacial Bone Regeneration by Modulating circAars/miR-128-3p/SMAD5 Signaling Axis

Synergistic effects of calcium silicate/zinc silicate dual compounds and in-situ interconnected pores on promoting bone regeneration of composite scaffolds

A self-assembling graphene oxide coating for enhanced bactericidal and osteogenic properties of poly-ether-ether-ketone

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