pH sensing and bioimaging using green synthesized carbon dots from black fungus

Qin, Jing; Gao, Xu; Chen, Qinqin; Liu, Huiling; Liu, Shuqi; Hou, Juan; Sun, Tiedong

doi:10.1039/d1ra05199g

Cited by 16 publications

(4 citation statements)

References 24 publications

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“…The images of both cell lines revealed that the CNPs have potential for bioimaging, since they had a zeta potential value of −24.10 (cationic) and a pH of 7.2, demonstrating their ability to penetrate into the cell membrane [ 47 , 48 , 49 , 50 ]. However, further investigations on the uptake of CNPs by individual cell organelles and nuclei are still required.…”

Section: Resultsmentioning

confidence: 99%

Carbon Nanoparticles Extracted from Date Palm Fronds for Fluorescence Bioimaging: In Vitro Study

Mohiuddin

Saeed

Alshahrie

et al. 2022

JFB

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Numerous studies have been reported on single- and multicolored highly fluorescent carbon nanoparticles (FCNPs) originating from various sources and their potential applications in bioimaging. Herein, multicolored biocompatible carbon nanoparticles (CNPs) unsheathed from date palm fronds were studied. The extracted CNPs were characterized via several microscopic and spectroscopic techniques. The results revealed that the CNPs were crystalline graphitic and hydrophilic in nature with sizes ranging from 4 to 20 nm. The unsheathed CNPs showed exemplary photoluminescent (PL) properties. They also emitted bright blue colors when exposed to ultraviolet (UV) light. Furthermore, in vitro cellular uptake and cell viability in the presence of CNPs were also investigated. The cell viability of human colon cancer (HCT-116) and breast adenocarcinoma (MCF-7) cell lines with aqueous CNPs at different concentrations was assessed by a cell metabolic activity assay (MTT) for 24 and 48 h incubations. The results were combined to generate dose-response curves for the CNPs and evaluate the severity of their toxicity. The CNPs showed adequate fluorescence with high cell viability for in vitro cell imaging. Under the laser-scanning confocal microscope, the CNPs with HCT-116 and MCF-7 cell lines showed multicolor fluorescence emissions, including blue, green, and red colors when excited at 405, 458, and 561 nm, respectively. These results prove that unsheathed CNPs from date palm fronds can be used in diverse biomedical applications because of their low cytotoxicity, adequate fluorescence, eco-friendly nature, and cheap production.

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Section: Resultsmentioning

confidence: 99%

Carbon Nanoparticles Extracted from Date Palm Fronds for Fluorescence Bioimaging: In Vitro Study

Mohiuddin

Saeed

Alshahrie

et al. 2022

JFB

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“…Several environmental and biological processes are influenced by pH, and changes in pH could reveal pollutants or diseases. CDs derived from black fungus demonstrated changes in fluorescence in solution in response to pH due to protonation-deprotonation of the surface functional groups [31]. As shown in figures 8(a) and (b), the fluorescence intensity increased as the pH increased from 1 to 4, and then gradually decreased in a linear fashion to pH 13.…”

Section: Ph Sensing Using Carbon Dotsmentioning

confidence: 91%

“…Furthermore, from ecological safety and sustainability perspectives, the synthesis of CDs from natural resources could mitigate environmental pollution and assist with waste management by repurposing agricultural waste into valueadded products. Other natural precursor materials include black fungus [31], bagasse, coffee grounds, banana juice, potatoes, leaves, palm shell powder, orange juice, vegetable waste and soybean juice, among others [32][33][34][35][36].…”

Section: Synthesis Of Carbon Dots From Organic Precursorsmentioning

confidence: 99%

Advances in the design and use of carbon dots for analytical and biomedical applications

Adeola,

Clermont-Paquette,

Piekny

et al. 2023

Nanotechnology

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Carbon dots (CDs) have garnered significant interest for their potential use in multiple applications due to their size, fluorescent properties, high photostability, low toxicity and biocompatibility. CDs can be tailored for specific needs, as they can be synthesized with diverse precursors and techniques for functionalization. Since the applications of CDs are rapidly expanding, this review highlights recent developments in this burgeoning field. Specifically, we describe advances in CD synthesis tailored for applications that include pH and temperature sensing, biochemical analysis, and bioimaging. We also discuss various challenges and practical solutions that will drive CD-based research forward. Challenges include the lack of standardized synthesis and purification methods for CDs, the lack of clarity regarding their mechanism of action, and procedural flaws in their applications. In conclusion, we provide recommendations for collaboration among disciplines to bridge existing knowledge gaps and address the key challenges required for CDs to be fully commercialized.

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“…The absorption peak at 280 nm can be attributed to a π-π* leap in the aromatic sp2 structural domain and has strong absorption, including C=C and C=O bonds. The C=O bond can also undergo an n-π* leap, which requires less energy and can have less strong absorption in the near-UV or visible region, while the absorption peak at 430 nm near the long-wave region is attributed to a red shift phenomenon where the maximum absorption peak is shifted towards the long-wave direction due to the influence of the substituent or solvent [38]. Polar solvents can shift the UV absorption peak in the long-wavelength direction.…”

Section: Optical Properties Of N-cqdsmentioning

confidence: 99%

Fluorescence Detection of Pb2+ in Environmental Water Using Biomass Carbon Quantum Dots Modified with Acetamide-Glycolic Acid Deep Eutectic Solvent

Xing,

Zheng,

Shi

et al. 2024

Molecules

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In this study, a novel green fluorescent probe material, nitrogen-doped carbon quantum dots (N-CQDs), was prepared by a one-step hydrothermal synthesis method using walnut green skin as a carbon source and acetamide-glycolic acid deep eutectic solvent (AGADES) as a modifier. By covalent coupling, the amide chromophore in AGADES is designed to cover the surface of walnut green skin carbon quantum dots (W-CQDs), forming a fluorescence energy resonance effect and improving the fluorescence performance of the carbon quantum dots. The prepared N-CQDs have a uniform particle size distribution, and the fluorescence quantum efficiency has increased from 12.5% to 32.5%. Within the concentration range of 0.01~1000 μmol/L of Pb2+, the linear detection limit is 1.55 nmol/L, which can meet the trace detection of Pb2+ in the water environment, and the recycling rate reaches 97%. This method has been successfully applied to the fluorescence detection and reuse of Pb2+ in actual water bodies, providing new ideas and methods for the detection of heavy metal ions in environmental water.

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pH sensing and bioimaging using green synthesized carbon dots from black fungus

Abstract: Carbon dots derived from black fungus were prepared and applied as a pH sensor for real water samples.

Cited by 16 publications

References 24 publications

Carbon Nanoparticles Extracted from Date Palm Fronds for Fluorescence Bioimaging: In Vitro Study

Carbon Nanoparticles Extracted from Date Palm Fronds for Fluorescence Bioimaging: In Vitro Study

Advances in the design and use of carbon dots for analytical and biomedical applications

Fluorescence Detection of Pb2+ in Environmental Water Using Biomass Carbon Quantum Dots Modified with Acetamide-Glycolic Acid Deep Eutectic Solvent

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