Phaeobacter inhibens as probiotic bacteria in non-axenic Artemia and algae cultures

Grotkjær, Torben; Bentzon-Tilia, Mikkel; D’Alvise, Paul; Dierckens, Kristof; Bossier, Peter; Gram, Lone

doi:10.1016/j.aquaculture.2016.05.001

Cited by 35 publications

(38 citation statements)

References 45 publications

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“…The efficient inhibition of vibrios by TDA‐producing roseobacters have been repeatedly demonstrated in studies targeting the fish pathogenic Vibrio spp. in marine eukaryotes (Porsby et al ., ; Prado et al ., ; D'Alvise et al ., ; Grotkjær et al ., ,b; Porsby and Gram, ; Rasmussen et al ., ). The high degree of target organism specificity is however somewhat surprising considering the spectrum of bioactivity of TDA (Porsby et al ., ).…”

Section: Discussionmentioning

confidence: 99%

Impact of Phaeobacter inhibens on marine eukaryote‐associated microbial communities

Dittmann

Sonnenschein

Egan

et al. 2018

Environ Microbiol Rep

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Summary Bacteria–host interactions are universal in nature and have significant effects on host functionality. Bacterial secondary metabolites are believed to play key roles in such interactions as well as in interactions within the host‐associated microbial community. Hence, prominent secondary metabolite‐producing bacteria may be strong drivers of microbial community composition in natural host‐associated microbiomes. This has, however, not been rigorously tested, and the purpose of this study was to investigate how the secondary metabolite producer Phaeobacter inhibens affects the diversity and composition of microbiomes associated with the microalga Emiliania huxleyi and the European flat oyster, Ostrea edulis. Roseobacters were indigenous to both communities exhibiting relative abundances between 2.8% and 7.0%. Addition of P. inhibens caused substantial changes in the overall structure of the low‐complexity microbiome of E. huxleyi, but did not shape microbial community structure to the same degree in the more complex oyster microbiomes. Species‐specific interactions occurred in both microbiomes and specifically the abundances of other putative secondary metabolite‐producers such as vibrios and pseudoalteromonads were reduced. Thus, the impact of a bioactive strain like P. inhibens on host‐associated microbiomes depends on the complexity and composition of the existing microbiome.

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Section: Discussionmentioning

confidence: 99%

Impact of Phaeobacter inhibens on marine eukaryote‐associated microbial communities

Dittmann

Sonnenschein

Egan

et al. 2018

Environ Microbiol Rep

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“…The mechanism behind this impact remains unclear, but we note that both of the probiotic species used in this study have been shown to inhibit Vibrio growth in vitro ( 31 – 33 ). P. inhibens is a particularly strong inhibitor of Vibrio growth and its use as an effective aquaculture probiotic has been advocated ( 34 , 35 ). The strain produces the quorum quenching molecule n-acyl homoserine lactone (AHL) and the antibiotic tropodithietic acid (TDA), both of which are highly effective against Vibrio virulence and growth ( 31 , 36 , 37 ).…”

Section: Discussionmentioning

confidence: 99%

Subtle Microbiome Manipulation Using Probiotics Reduces Antibiotic-Associated Mortality in Fish

et al. 2017

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Prophylactic antibiotics are widespread in the aquaculture industry and are used where vaccination is impossible or overly expensive. If antibiotics impact fish as they do mice and humans, prophylactic administrations in aquaculture and ornamental fish farms may increase downstream disease susceptibility in target hosts, despite short-term pathogen control benefits. Recent research has suggested that their use exacerbates bacterial outbreaks by creating sterile, nutrient-rich environments for invading pathogens to colonize and could help to explain rising economic costs of bacterial outbreaks in aquaculture. Our findings suggest a long-term cost of prophylactic antibiotic use and demonstrate a probiotic-based solution that does not rely on full microbiome community transplantation.

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“…Members of the Roseobacter clade have shown great potential as antagonists of fish pathogens in the marine larviculture environment including live feed cultures (D'Alvise et al ., ; Grotkjær et al ., ,b) and in larvae of turbot and cod (Hjelm et al ., ; D'Alvise et al ., ). Their probiotic effect does probably not rely on quorum‐sensing inhibition, but rather on the production of one or more antimicrobial compounds including tropodithietic acid (TDA), indigoidine, tryptanthrin and dicyclic peptides (Bentzon‐Tilia and Gram, in press).…”

Section: Microbial Control Of Fish Diseasesmentioning

confidence: 99%

“…Roseobacters are indigenous to the aquaculture environment (Bentzon‐Tilia and Gram, in press, and references herein), and should hence possess the overall abilities to remain in the systems. Steps towards addressing the efficacy of Roseobacter probiotics in live feed systems in the presence of aquaculture‐relevant microbial communities have been taken (Grotkjær et al ., ), yet whether the probionts added to live feed will remain and proliferate downstream in the system remain unknown. By tracing the probionts in different up‐scaling scenarios, this question can be addressed and such studies would provide valuable data for developing and optimizing administration procedures.…”

Section: Microbial Control Of Fish Diseasesmentioning

confidence: 99%

Monitoring and managing microbes in aquaculture – Towards a sustainable industry

Bentzon-Tilia

Sonnenschein

Gram

2016

Microbial Biotechnology

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SummaryMicroorganisms are of great importance to aquaculture where they occur naturally, and can be added artificially, fulfilling different roles. They recycle nutrients, degrade organic matter and, occasionally, they infect and kill the fish, their larvae or the live feed. Also, some microorganisms may protect fish and larvae against disease. Hence, monitoring and manipulating the microbial communities in aquaculture environments hold great potential; both in terms of assessing and improving water quality, but also in terms of controlling the development of microbial infections. Using microbial communities to monitor water quality and to efficiently carry out ecosystem services within the aquaculture systems may only be a few years away. Initially, however, we need to thoroughly understand the microbiomes of both healthy and diseased aquaculture systems, and we need to determine how to successfully manipulate and engineer these microbiomes. Similarly, we can reduce the need to apply antibiotics in aquaculture through manipulation of the microbiome, i.e. by the use of probiotic bacteria. Recent studies have demonstrated that fish pathogenic bacteria in live feed can be controlled by probiotics and that mortality of infected fish larvae can be reduced significantly by probiotic bacteria. However, the successful management of the aquaculture microbiota is currently hampered by our lack of knowledge of relevant microbial interactions and the overall ecology of these systems.

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Phaeobacter inhibens as probiotic bacteria in non-axenic Artemia and algae cultures

Cited by 35 publications

References 45 publications

Impact of Phaeobacter inhibens on marine eukaryote‐associated microbial communities

Impact of Phaeobacter inhibens on marine eukaryote‐associated microbial communities

Subtle Microbiome Manipulation Using Probiotics Reduces Antibiotic-Associated Mortality in Fish

Monitoring and managing microbes in aquaculture – Towards a sustainable industry

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