Phage Display Methods for Detection of Bacterial Pathogens

Gulig, Paul A.; Martin, Jay F.; Messer, Harald; Deffense, Beverly L.; Harpley, Crystal J.

doi:10.1007/978-0-387-75113-9_28

Cited by 6 publications

(2 citation statements)

References 85 publications

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“…Phage display, while not as prevalent as antibodies, still has generated considerable interest for use in biothreat sensors . Recent advances in this area have seen phage used for the generation of peptide- and antibody-based capture sequences to a number of biothreat pathogens including B. anthracis cells, toxins, , spores, Brucella spp., Burkholderia spp., and Salmonella .…”

Section: Biochemical Sensorsmentioning

confidence: 99%

Detecting Biothreat Agents: From Current Diagnostics to Developing Sensor Technologies

et al. 2018

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Although a fundamental understanding of the pathogenicity of most biothreat agents has been elucidated and available treatments have increased substantially over the past decades, they still represent a significant public health threat in this age of (bio)terrorism, indiscriminate warfare, pollution, climate change, unchecked population growth, and globalization. The key step to almost all prevention, protection, prophylaxis, post-exposure treatment, and mitigation of any bioagent is early detection. Here, we review available methods for detecting bioagents including pathogenic bacteria and viruses along with their toxins. An introduction placing this subject in the historical context of previous naturally occurring outbreaks and efforts to weaponize selected agents is first provided along with definitions and relevant considerations. An overview of the detection technologies that find use in this endeavor along with how they provide data or transduce signal within a sensing configuration follows. Current "gold" standards for biothreat detection/diagnostics along with a listing of relevant FDA approved in vitro diagnostic devices is then discussed to provide an overview of the current state of the art. Given the 2014 outbreak of Ebola virus in Western Africa and the recent 2016 spread of Zika virus in the Americas, discussion of what constitutes a public health emergency and how new in vitro diagnostic devices are authorized for emergency use in the U.S. are also included. The majority of the Review is then subdivided around the sensing of bacterial, viral, and toxin biothreats with each including an overview of the major agents in that class, a detailed cross-section of different sensing methods in development based on assay format or analytical technique, and some discussion of related microfluidic lab-on-a-chip/point-of-care devices. Finally, an outlook is given on how this field will develop from the perspective of the biosensing technology itself and the new emerging threats they may face.

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Section: Biochemical Sensorsmentioning

confidence: 99%

Detecting Biothreat Agents: From Current Diagnostics to Developing Sensor Technologies

et al. 2018

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“…The main biological sensing materials used in biosensor development are the couple antibody/antigen [201]. Yet, a number of other recognition elements are currently used, which include nucleic acid diagnostics [202], aptamers [203], molecularly imprinted polymers [204], phage display methods [205], bacteriophages [206,207]. An excellent representation of bacterial sensors and a review of progress of bacteriophage use in bacterial sensors are presented by Singh et al [208], where the majority of types of sensors, including that of nucleic acids and antibodies are presented, in addition to bacteriophages, and tables of sensors available for specific microorganisms.…”

Section: Biosensorsmentioning

confidence: 99%

Current and Future Trends in the Clinical Diagnosis of Rickettsioses Sensu Lato

Missailidis

Godoy²,

Mares-Guia³

et al. 2014

IJTDH

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Aims:The aim of this review is to present Rickettsioses sensu lato, with emphasis on their current and future clinical diagnosis. The review presents the conditions, the agents that cause them, and the current gold standards on their diagnosis in national and international reference centres. Additionally, this review covers the various emerging technologies available in the diagnosis of Rickettsioses and discusses their potential for future use as gold standards in the diagnosis of these diseases. Introduction: The introduction presents Rickettsioses sensu lato and gives a broad overview of the conditions they cause, the issues associated with their current diagnosis and the need for their improved, earlier and more accurate diagnosis, in order to prevent current issues with false negatives, misdiagnosis or delay in the diagnosis associated with these conditions, which often renders them grave or lethal. Main Body: The main body of the review presents in independent sections Rickettsias, Ehrlichia, Anaplasma, Bartonella and Coxiella and the conditions associated with each of these bacteria. Spotted fever, endemic typhus, human granulocytic anaplasmosis, human monocytic ehrlichioses, bartonellosis and Q-fever are some of the conditions associated with this group of proteobacteria. The emphasis is on the clinical diagnosis of these conditions and an overview of the current practice, gold standards in reference

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Grading the commercial optical biosensor literature—Class of 2008: ‘The Mighty Binders’

Rich

Myszka

2009

J of Molecular Recognition

143

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Optical biosensor technology continues to be the method of choice for label-free, real-time interaction analysis. But when it comes to improving the quality of the biosensor literature, education should be fundamental. Of the 1413 articles published in 2008, less than 30% would pass the requirements for high-school chemistry. To teach by example, we spotlight 10 papers that illustrate how to implement the technology properly. Then we grade every paper published in 2008 on a scale from A to F and outline what features make a biosensor article fabulous, middling or abysmal. To help improve the quality of published data, we focus on a few experimental, analysis and presentation mistakes that are alarmingly common. With the literature as a guide, we want to ensure that no user is left behind.

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Phage Display Methods for Detection of Bacterial Pathogens

Cited by 6 publications

References 85 publications

Detecting Biothreat Agents: From Current Diagnostics to Developing Sensor Technologies

Detecting Biothreat Agents: From Current Diagnostics to Developing Sensor Technologies

Current and Future Trends in the Clinical Diagnosis of Rickettsioses Sensu Lato

Grading the commercial optical biosensor literature—Class of 2008: ‘The Mighty Binders’

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