Phage display selection of peptides that inhibit metastasis ability of gastric cancer cells with high liver-metastatic potential

Hu, Shengjuan; Guo, Xin-ning; Xie, Hui; Du, Yulei; Pan, Yanglin; Shi, Yongquan; Wang, Jun; Hong, Liu; Han, Sheng; Zhang, Dongtao; Huang, Dawei; Zhang, Kedong; Bai, Feihu; Jiang, Haiping; Zhai, Huihong; Nie, Yongzhan; Wu, Kaichun; Fan, Daiming

doi:10.1016/j.bbrc.2006.01.047

Cited by 35 publications

(16 citation statements)

References 20 publications

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“…A human gastric cancer cell line, GC9811, was kept in our lab [8,9]. The cells were maintained in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS), pH 7.2, in a humidified atmosphere of 95% air and 5% CO 2 at 37 • C. Cells were passaged and expanded by trypsinization of cell monolayers followed by replating every 3-4 days.…”

Section: Cell Lines and Cell Culturementioning

confidence: 99%

“…The adhesion effect of extracellular matrix (ECM) components was examined in GC9811 cells and GC9811-P cells according to the adhesion assay method as described previously [8]. Briefly, 4 × 10 4 cells were added to each well and incubated for 1 hr, and then the unattached cells were removed and attached cells were estimated from the absorbance at 490 nm.…”

Section: Evaluation Of In Vitro Cell Adhesionmentioning

confidence: 99%

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Establishment and Characterization of a High Metastatic Potential in the Peritoneum for Human Gastric Cancer by Orthotopic Tumor Cell Implantation

et al. 2007

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The aim of this study was to establish an orthotopic implantation model with high metastasis of gastric cancer to the peritoneum which is more faithful to clinical metastasis. A human gastric carcinoma cell line, GC9811, was injected as a single-cell suspension into the stomach of nude mice. The cells from some peritoneum metastatic foci were expanded in vitro and subsequently implanted to the stomach wall of nude mice. By repeating the in vivo stepwise selection method for four rounds and cloning culture, we obtained a cell line designated GC9811-P, which developed peritoneal metastasis in 13 of 13 (100%) of mice, compared with only 20% of those implanted with parental GC9811. The metastatic foci in the peritoneum showed essentially the same histological appearance as those induced by parental cells. Tumor cell growth of GC9811-P in vitro was faster than that of GC9811. Motility assays demonstrated higher motility of GC9811-P than of GC9811. The adhesive ability of GC9811-P cells to laminin was lower than that of GC9811 cells, whereas the ability of GC9811-P cells to adhere to fibronectin was significantly higher than that of parental cells. Differences between GC9811-P and their parental GC9811 cells were found in expression levels of various molecules by flow cytometric and western blot. The findings indicated that up-regulation in the expressions of CD155, VEGF, syndecan-1, and syndecan-2 or down-regulation in the expressions of IL-6 and E-cadherin play an important role in the peritoneal metastasis of human gastric carcinoma cells. The high-metastatic cell line appears to be useful for investigating the mechanisms of peritoneal metastasis and preventing peritoneal metastasis of human gastric cancer.

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Section: Cell Lines and Cell Culturementioning

confidence: 99%

Section: Evaluation Of In Vitro Cell Adhesionmentioning

confidence: 99%

Establishment and Characterization of a High Metastatic Potential in the Peritoneum for Human Gastric Cancer by Orthotopic Tumor Cell Implantation

et al. 2007

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“…[6][7][8] The flagella peptide library screening can help to overcome some disadvantages associated with the traditional target therapies. [9][10][11][12][13] In our previous studies, TMTP1, obtained by using the flagella peptide library screening, could target malignant tumor in situ lesions, metastases and even micro-metastases.…”

Section: Discussionmentioning

confidence: 99%

TMTP1, a novel tumor-homing peptide, specifically targets hematological malignancies and their metastases

Xiao

Hong

Sun

et al. 2011

J. Huazhong Univ. Sci. Technol. [Med. Sci.]

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TMTP1, a 5-amino acid peptide NVVRQ, obtained by using the flagella peptide library screening in our previous studies, can be used for the labeling of malignant in situ and metastatic lesions, and even micro-metastases. In this study, TMTP1 was assessed for its ability to specifically target the malignant hematopoietic cells and metastatic lesions of hematological malignancies. FITC-TMTP1 was chemically synthesized. Immunofluorescence assay and competitive test were carried out to determine the specific binding capacity of TMTPl to hematological malignant cell lines, including HL60, k562, SHI-1, Jurkat, Raji, El-4 and umbilical cord blood mononuclear cells. Mononuclear cells were isolated from the bone marrow of healthy subjects and patients with chronic myeloid leukemia. Then the cells were co-cultured with TMTP1 or scrambled peptides and the binding and affinity of TMTP1 peptide to the primary cells of hematological malignancies were flow cytometrically analyzed. The binding specificity of TMTP1 to target hematological malignancies was measured in vivo by intravenous injection of FITC-conjugated TMTP1 into El-4 lymphoma-bearing mice. The results showed that TMTP1 specifically bound to the cells of a series of hematological malignancies, including HL60, k562, Jurkat, Raji, El-4 and chronic myeloid leukemia primary cells but not to bone marrow mononuclear cells from healthy subjects. By contrast, TMTP1 could bind to the metastatic foci of lymphoma originating from the EL-4 cell line while the scrambled peptide failed to do so. Moreover, the occult metastases could be identified, with high specificity, by detecting FITC-TMTP1. We are led to conclude that TMTP1, as a novel tumor-homing peptide, can serve as a marker for primary malignant and metastatic lesions for the early diagnosis of hematological malignances and a carrier of anticancer drugs for cancer treatment.

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“…Several cancer-specific peptides have demonstrated induction of these pathways. 110,126,140,154,160,163,169,170,251,252 Not surprisingly, peptides that bind integrins are often found to inhibit adhesion and migration as this family of receptors are critical for such functions, yet many other ligands are able to block cellular migration. For example, a peptide isolated from phage display against MMP-9 inhibited migration of various tumor cells (fibrosarcoma, melanoma, ovarian carcinoma, and Kaposi’s sarcoma cells) in vitro and inhibited Kaposi’s sarcoma tumor growth when ip injected in vivo.…”

Section: Peptides With Cellular Effectsmentioning

confidence: 99%

“…170 Another peptide selected against metastatic gastric cancer cells inhibited adhesion and migration in vitro and reduced liver metastasis incidence in vivo. 169 …”

Section: Peptides With Cellular Effectsmentioning

confidence: 99%

Combinatorial Peptide Libraries: Mining for Cell-Binding Peptides

2013

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Phage display selection of peptides that inhibit metastasis ability of gastric cancer cells with high liver-metastatic potential

Cited by 35 publications

References 20 publications

Establishment and Characterization of a High Metastatic Potential in the Peritoneum for Human Gastric Cancer by Orthotopic Tumor Cell Implantation

Establishment and Characterization of a High Metastatic Potential in the Peritoneum for Human Gastric Cancer by Orthotopic Tumor Cell Implantation

TMTP1, a novel tumor-homing peptide, specifically targets hematological malignancies and their metastases

Combinatorial Peptide Libraries: Mining for Cell-Binding Peptides

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