Phage Display: Simple Evolution in a Petri Dish (Nobel Lecture)

Smith, George P.

doi:10.1002/anie.201908308

Cited by 96 publications

(67 citation statements)

References 36 publications

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“…Here we described a droplet-based microfluidics platform for functional screening of millions of antibodies. The platform shared some key features with the most efficient selection methods such as phage display (8)(9)(10)(11)(12)(13). First, the genotype and phenotype linkage was maintained through the whole process.…”

Section: Discussionmentioning

confidence: 99%

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High throughput functional screening for next generation cancer immunotherapy using droplet-based microfluidics

Wang

Jin

Shen³

et al. 2020

Preprint

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Currently high throughput approaches are lagged for isolation of antibodies whose function goes beyond simple binding, which have prevented the next generation cancer immunotherapeutics, such as bispecific T cell engager antibodies or agonist antibody of costimulatory receptor, from reaching their full potential. Here we developed a highly efficient droplet-based microfluidics platform combining with lentivirus transduction system that enables functional screening of millions of antibodies. To showcase the capacity of the system, functional antibodies for CD40 agonism with low frequency (<0.02%) were identified with 2 rounds of screening. To demonstrate its versatility, an anti-Her2/anti-CD3 bispecific antibody library was established using bispecific T cell Engager (BiTE) platform and functional screening enabled efficient identification of potent anti-Her2/anti-CD3 BiTE antibodies. The platform could revolutionize the next generation cancer immunotherapy drug development and research world.

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Section: Discussionmentioning

confidence: 99%

“…The in vitro display technology, such as phage display, allows to select antibody binders from large combinatorial library, with the capacity of 10 11 diversities (8)(9)(10)(11)(12)(13) and has been one of the major players in conventional antibody drug development by the use of high or low throughput simple binding assays.…”

Section: Introductionmentioning

confidence: 99%

High throughput functional screening for next generation cancer immunotherapy using droplet-based microfluidics

Wang

Jin

Shen³

et al. 2020

Preprint

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“…Natural evolution provided us with a method to carry out this screening process in vitro [68]. Several screening methods inspired by this mechanism made it possible to identify specific molecular binders from a library, experimentally [69].…”

Section: Novel Materials Based On High-throughput Selection Methodsmentioning

confidence: 99%

“…Phage display is a laboratory technique that enables the selection of peptides or proteins with a high affinity for a target [68]. It uses bacteriophages, viruses that infect bacteria.…”

Section: Phage Displaymentioning

confidence: 99%

Bio-Inspired Strategies for Improving the Selectivity and Sensitivity of Artificial Noses: A Review

Hurot

Scaramozzino

Buhot

et al. 2020

Sensors

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Artificial noses are broad-spectrum multisensors dedicated to the detection of volatile organic compounds (VOCs). Despite great recent progress, they still suffer from a lack of sensitivity and selectivity. We will review, in a systemic way, the biomimetic strategies for improving these performance criteria, including the design of sensing materials, their immobilization on the sensing surface, the sampling of VOCs, the choice of a transduction method, and the data processing. This reflection could help address new applications in domains where high-performance artificial noses are required such as public security and safety, environment, industry, or healthcare.

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“…The technique of phage display ( Figure 1B) is an important tool for the identification of protein-protein interactions. [6][7][8][9][10][11][12][13][14][15] The process begins with insertion of a DNA sequence that encodes a particular protein into the coat protein gene of a bacteriophage. The protein is expressed on the surface of the bacteriophage.…”

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confidence: 99%

DNA Encoded Libraries: A Visitor's Guide

Flood

Kingston

Vantourout

et al. 2020

Israel Journal of Chemistry

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In 1992, Brenner and Lerner hypothesized that individual chemical transformations could be encoded in DNA, allowing the rapid synthesis and screening of large collections of small molecules. Since their report, huge investments into the development of the DNA encoded library (DEL) technology have enabled the acceleration of the drug discovery process especially early phase discovery undertakings such as target validation and hit identification. As DEL lies at the nexus between chemistry and biology, there is an increasing need to expand the toolboxes of both organic transformations and biological methods. However, the myriad of techniques and reactions already reported can be difficult to digest for practitioners whose expertise resides outside the realm of DEL. This review therefore focuses on a stepwise presentation of DEL from the basic concepts to newest developments. The presentation includes the history, fundamentals, and successes of DEL, different methods for DEL synthesis and affinity selection, the conventional transformations, and finally the latest developments from a synthetic organic perspective.

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Phage Display: Simple Evolution in a Petri Dish (Nobel Lecture)

Cited by 96 publications

References 36 publications

High throughput functional screening for next generation cancer immunotherapy using droplet-based microfluidics

High throughput functional screening for next generation cancer immunotherapy using droplet-based microfluidics

Bio-Inspired Strategies for Improving the Selectivity and Sensitivity of Artificial Noses: A Review

DNA Encoded Libraries: A Visitor's Guide

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