Phage-Displayed Nanobody as a Sensitive Nanoprobe to Enhance Chemiluminescent Immunoassay for <i>Cronobacter sakazakii</i> Detection in Dairy Products

Zhang, Yao; Liao, Xingrui; Yu, Gege; Wei, Juan; Wang, Peng; Wang, Yueqi; Jing, Yinnan; Wang, Jiamin; Chen, Pengyu; Wang, Jianlong; Wang, Hong; Wang, Yanru

doi:10.1021/acs.analchem.3c02990

Cited by 9 publications

(2 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Foodborne gastrointestinal bacterial pathogens are one of the major foodborne pathogenic bacteria causing human diarrheal diseases, such as diarrhea, abdominal cramping, and vomiting. − Foodborne pathogens are widely polluted, spread swiftly, and do excellent harm, which has a great impact on human health and the economy and has turned out to be a premier public health problem in the world. , Among them, Salmonella typhimurium (S. typhimurium) is a particularly notorious intestinal pathogen, which is associated with the outbreak of numerous foodborne diseases. , The incidence rate of S.…”

Section: Introductionmentioning

confidence: 99%

Dual-Readout Ultrasensitive Lateral Flow Immunosensing of Salmonella typhimurium in Dairy Products by Doping Engineering-Powered Nanoheterostructure with Enhanced Photothermal Performance

Shi,

Wang,

et al. 2024

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

The photothermal lateral flow immunoassay (LFIA) is of great significance to suitable for on-site semiquantitative detection, which has the upper hand in further constructing detection methods for low-concentration targets. Herein, we presented a doping engineering-powered nanoheterostructure with an enhanced photothermal performance strategy, employing bimetallic nanocuboid Pt 3 Sn (PSNCs) as a proof of concept. With the help of finite element simulation analysis, the contrast of direct temperature experiment, and the evaluation of photothermal conversion efficiency (η), the distinguished and enthusiastic photothermal feedback of PSNCs is proved. Based on steady bright black of colorimetric and superior photothermal performance, the PSNCs were employed to construct an ultrasensitive model LIFA for detecting Salmonella typhimurium (S. typhimurium), which achieved the double-signal semiquantitative detection, the detection limit reached 10 3 cfu mL −1 (colorimetric mode) and 10 2 cfu mL −1 (photothermal mode), which is 100 times higher than that of the traditional colloidal gold method. In addition, the method was effective for the detection of targets in dairy samples only through a simple dilution treatment, which was completed within 15 min. Meanwhile, this PSNCs dual-signal LFIA demonstrated the sensitive detection of S. typhimurium due to the excellent colorimetric signal and significant photothermal performance, which provides a broad spectrum for the future detection of foodborne pathogens.

show abstract

Section: Introductionmentioning

confidence: 99%

Dual-Readout Ultrasensitive Lateral Flow Immunosensing of Salmonella typhimurium in Dairy Products by Doping Engineering-Powered Nanoheterostructure with Enhanced Photothermal Performance

Shi,

Wang,

et al. 2024

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Compared with traditional antibodies, M13 phage-screened peptides have several advantages: (1) The major capsid protein (pVIII) of the phage serves as a scaffold, which can be recognized by thousands of M13 phage antibody-conjugated horseradish peroxidase (HRP) (anti-M13-HRP), helping to enhance signaling. (2) The biopanned phages are easy to prepare and purify, ready for scale-up application . Therefore, phage display has been widely used for virus-specific ligand screening, , disease diagnosis, − drug development, bioanalysis, and biosensing. , …”

Section: Introductionmentioning

confidence: 99%

Sensitive Dual-Signal ELISA Based on Specific Phage-Displayed Double Peptide Probes with Internal Filtering Effect to Assay Monkeypox Virus Antigen

Pang,

Wang,

Yuan

et al. 2024

Anal. Chem.

View full text Add to dashboard Cite

The global spread of monkeypox has become a worldwide public healthcare issue. Therefore, there is an urgent need for accurate and sensitive detection methods to effectively control its spreading. Herein, we screened by phage display two peptides M4 (sequence: DPCGERICSIAL) and M6 (sequence: SCSSFLCSLKVG) with good affinity and specificity to monkeypox virus (MPXV) B21R protein. To simulate the state of the peptide in the phage and to avoid spatial obstacles of the peptide, GGGSK was added at the C terminus of M4 and named as M4a. Molecular docking shows that peptide M4a and peptide M6 are bound to different epitopes of B21R by hydrogen bonds and salt-bridge interactions, respectively. Then, peptide M4a was selected as the capture probe, phage M6 as the detection probe, and carbonized polymer dots (CPDs) as the fluorescent probe, and a colorimetric and fluorescent double-signal capture peptide/antigen/signal peptidedisplayed phage sandwich ELISA triggered by horseradish peroxidase (HRP) through a simple internal filtration effect (IFE) was constructed. HRP catalyzes H 2 O 2 to oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to generate blue oxidized TMB, which can further quench the fluorescence of CPDs through IFE, enabling to detect MPXV B21R in colorimetric and fluorescent modes. The proposed simple immunoassay platform shows good sensitivity and reliability in MPXV B21R detection. The limit of detection for colorimetric and fluorescent modes was 27.8 and 9.14 pg/mL MPXV B21R, respectively. Thus, the established double-peptide sandwich-based dual-signal immunoassay provides guidance for the development of reliable and sensitive antigen detection capable of mutual confirmation, which also has great potential for exploring various analytical strategies for other respiratory virus surveillance.

show abstract

Enzyme-based colorimetric signal amplification strategy in lateral flow immunoassay

Gong,

Zeng,

Gai

et al. 2024

Chinese Chemical Letters

View full text Add to dashboard Cite

Phage-Displayed Nanobody as a Sensitive Nanoprobe to Enhance Chemiluminescent Immunoassay for Cronobacter sakazakii Detection in Dairy Products

Cited by 9 publications

References 32 publications

Dual-Readout Ultrasensitive Lateral Flow Immunosensing of Salmonella typhimurium in Dairy Products by Doping Engineering-Powered Nanoheterostructure with Enhanced Photothermal Performance

Dual-Readout Ultrasensitive Lateral Flow Immunosensing of Salmonella typhimurium in Dairy Products by Doping Engineering-Powered Nanoheterostructure with Enhanced Photothermal Performance

Sensitive Dual-Signal ELISA Based on Specific Phage-Displayed Double Peptide Probes with Internal Filtering Effect to Assay Monkeypox Virus Antigen

Enzyme-based colorimetric signal amplification strategy in lateral flow immunoassay

Contact Info

Product

Resources

About