Phage long tail fiber protein-immobilized magnetic nanoparticles for rapid and ultrasensitive detection of Salmonella

Wang, Luokai; Lin, Hong; Zhang, Jing; Wang, Jingxue

doi:10.1016/j.talanta.2022.123627

Cited by 26 publications

(4 citation statements)

References 32 publications

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“…Briefly, the probe PhageT102‐MBs recovery was performed as described in Section 2.3. The bead‐cell complexes were resuspended in 25 μL TE buffer (Beyotime Biotechnology, Shanghai, China) and boiled at 100°C for 10 min to obtain the bacterial genomic DNA (L. Wang et al, 2022). The lysate (template) was harvested by centrifugation at 12,000× g at 4°C for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Phagomagnetic separation in combination with real‐time PCR assay for detection of Salmonella Enteritidis and Typhimurium in dairy products

Ding,

Li,

Huang

et al. 2023

Journal of Food Safety

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Salmonella Enteritidis (SE) and Salmonella Typhimurium (ST) are the most commonly reported serotypes detected for the occurrence of Salmonellosis through foodborne transmission in recent years. In this study, a phagomagnetic separation in combination with qPCR (PhMS‐qPCR) based on phage T102 as a recognition element was developed for rapid enrichment and detection of Salmonella in dairy products. Phage T102 was coated onto magnetic beads for capturing Salmonella from samples through magnetic separation, with a capture efficiency of ~90% demonstrated. During the subsequent qPCR process, captured Salmonella was identified by detecting the OmpC gene, and specific sdf and STM4495 genes to further distinguish the serotypes of Salmonella Enteritidis (SE) and Salmonella Typhimurium (ST), respectively. The whole procedure can be performed in 2.5 h with a low detection limit of 10 CFU/mL in PBS. Subsequently, this detection strategy was successfully applied for the detection of Salmonella and serotype identification in spiked milk samples. This cell separation and detection system offers a promising alternative for rapid and accurate detection of Salmonella or Salmonella spp.

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Section: Methodsmentioning

confidence: 99%

Phagomagnetic separation in combination with real‐time PCR assay for detection of Salmonella Enteritidis and Typhimurium in dairy products

Ding,

Li,

Huang

et al. 2023

Journal of Food Safety

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“…During cell separation, MNPs are bound to specific biorecognition molecules such as antigens, glycoproteins, and specific structural components on the cell surface by Magnetic Activated Cell Sorting (MACS) [ 110 , 111 , 112 ], nucleic acid probes, antibiotics, phages [ 113 ], and lectins [ 114 , 115 ]. Subsequently, the MNP-labeled cells are separated from the mixtures through the application of a magnetic field, leading to the enrichment and purification of the target cells.…”

Section: Applications Of Mnps In Biomedical Fieldsmentioning

confidence: 99%

The Recent Applications of Magnetic Nanoparticles in Biomedical Fields

Hong,

Wang,

Zheng

et al. 2024

Materials

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Magnetic nanoparticles (MNPs) have found extensive application in the biomedical domain due to their enhanced biocompatibility, minimal toxicity, and strong magnetic responsiveness. MNPs exhibit great potential as nanomaterials in various biomedical applications, including disease detection and cancer therapy. Typically, MNPs consist of a magnetic core surrounded by surface modification coatings, such as inorganic materials, organic molecules, and polymers, forming a nucleoshell structure that mitigates nanoparticle agglomeration and enhances targeting capabilities. Consequently, MNPs exhibit magnetic responsiveness in vivo for transportation and therapeutic effects, such as enhancing medical imaging resolution and localized heating at the site of injury. MNPs are utilized for specimen purification through targeted binding and magnetic separation in vitro, thereby optimizing efficiency and expediting the process. This review delves into the distinctive functional characteristics of MNPs as well as the diverse bioactive molecules employed in their surface coatings and their corresponding functionalities. Additionally, the advancement of MNPs in various applications is outlined. Additionally, we discuss the advancements of magnetic nanoparticles in medical imaging, disease treatment, and in vitro assays, and we anticipate the future development prospects and obstacles in this field. The objective is to furnish readers with a thorough comprehension of the recent practical utilization of MNPs in biomedical disciplines.

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“…Researchers have also explored utilizing bacteriophage-derived affinity molecules as recognition elements rather than the entire bacteriophage structures. Bacteriophage lysin (LysGH15) and long tail fibers have been recently incorporated as recognition elements to detect S. aureus and Salmonella spp., respectively ( Liu et al, 2022 ; Wang et al, 2022a ). LysGH15 lost its lytic activity through C54A mutation but retained its recognizing and binding ability to S. aureus .…”

Section: Bacteriophage-based Biosensorsmentioning

confidence: 99%

“…The BLI-based method was able to detect S. aureus whole cells directly in food samples (ice cube and light soy sauce) with a LOD of 13 CFU/ml with a binding time of 12 min. Bacteriophage long-tail-fiber proteins (LTF4-a) were immobilized onto magnetic nanoparticles (MNPs) for specific separation and concentration of S. Typhimurium ( Wang et al, 2022a ). LTF4-a-MNP complex allowed the isolation and enrichment of Salmonella cells from artificially spiked food samples (milk, lettuce, and egg) prior to direct qPCR.…”

Section: Bacteriophage-based Biosensorsmentioning

confidence: 99%

Advances, applications, and limitations of portable and rapid detection technologies for routinely encountered foodborne pathogens

et al. 2022

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Traditional foodborne pathogen detection methods are highly dependent on pre-treatment of samples and selective microbiological plating to reliably screen target microorganisms. Inherent limitations of conventional methods include longer turnaround time and high costs, use of bulky equipment, and the need for trained staff in centralized laboratory settings. Researchers have developed stable, reliable, sensitive, and selective, rapid foodborne pathogens detection assays to work around these limitations. Recent advances in rapid diagnostic technologies have shifted to on-site testing, which offers flexibility and ease-of-use, a significant improvement from traditional methods’ rigid and cumbersome steps. This comprehensive review aims to thoroughly discuss the recent advances, applications, and limitations of portable and rapid biosensors for routinely encountered foodborne pathogens. It discusses the major differences between biosensing systems based on the molecular interactions of target analytes and biorecognition agents. Though detection limits and costs still need further improvement, reviewed technologies have high potential to assist the food industry in the on-site detection of biological hazards such as foodborne pathogens and toxins to maintain safe and healthy foods. Finally, this review offers targeted recommendations for future development and commercialization of diagnostic technologies specifically for emerging and re-emerging foodborne pathogens.

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Phage long tail fiber protein-immobilized magnetic nanoparticles for rapid and ultrasensitive detection of Salmonella

Cited by 26 publications

References 32 publications

Phagomagnetic separation in combination with real‐time PCR assay for detection of Salmonella Enteritidis and Typhimurium in dairy products

Phagomagnetic separation in combination with real‐time PCR assay for detection of Salmonella Enteritidis and Typhimurium in dairy products

The Recent Applications of Magnetic Nanoparticles in Biomedical Fields

Advances, applications, and limitations of portable and rapid detection technologies for routinely encountered foodborne pathogens

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