Phage tail fibre assembly proteins employ a modular structure to drive the correct folding of diverse fibres

North, Olesia I.; Sakai, Kazuhisa; Yamashita, Eiki; Nakagawa, Atsushi; Iwazaki, Takuma; Buttner, C.R.; Takeda, Shigeki; Davidson, Alan R.

doi:10.1038/s41564-019-0477-7

Cited by 59 publications

(54 citation statements)

References 45 publications

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“…Structural sequence analysis predicted that the depolymerase activity of these phages resides in tailspikes. Tail fibers and tailspikes are responsible of specific recognition of bacterial surfaces [18], and depolymerases have been shown to participate in this process [19]. The fact that all four phages shared similar depolymerases may explain why they infect the same capsular type.…”

Section: Discussionmentioning

confidence: 99%

Isolation of Four Lytic Phages Infecting Klebsiella pneumoniae K22 Clinical Isolates from Spain

Domingo‐Calap

Beamud

Vienne

et al. 2020

IJMS

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The emergence of multi-drug-resistant bacteria represents a major public-health threat. Phages constitute a promising alternative to chemical antibiotics due to their high host specificity, abundance in nature, and evolvability. However, phage host specificity means that highly diverse bacterial species are particularly difficult to target for phage therapy. This is the case of Klebsiella pneumoniae, which presents a hypervariable extracellular matrix capsule exhibiting dozens of variants. Here, we report four novel phages infecting K. pneumoniae capsular type K22 which were isolated from environmental samples in Valencia, Spain. Full genome sequencing showed that these phages belong to the Podoviridae family and encode putative depolymerases that allow digestion of specific K22 K. pneumoniae capsules. Our results confirm the capsular type-specificity of K. pneumoniae phages, as indicated by their narrow infectivity in a panel of K. pneumoniae clinical isolates. Nonetheless, this work represents a step forward in the characterization of phage diversity, which may culminate in the future use of large panels of phages for typing and/or for combating multi-drug-resistant K. pneumoniae.

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Section: Discussionmentioning

confidence: 99%

Isolation of Four Lytic Phages Infecting Klebsiella pneumoniae K22 Clinical Isolates from Spain

Domingo‐Calap

Beamud

Vienne

et al. 2020

IJMS

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“…Our evidence of ORF31 as being the putative tail fiber is based on the level of structural similarity to tail fiber of bacteriophage Mu G+. A recent crystal structure showed that the tail fiber and the chaperone of Mu form a complex and that both proteins are part of the mature virion [37]. However, none of the two chaperones of S144 were identified in the proteome analysis, suggesting that they are not part of the mature virion.…”

Section: Discussionmentioning

confidence: 99%

“…The genes for the tail fiber morphogenesis include the tail fiber protein (ORF31) and two chaperones (ORF32 and ORF33), which are predicted to be needed for tail fiber assembly, as both proteins showed high homology to GpU, the chaperone of the tail fiber protein of Mu G+ (HHPRED probability 100%, e-value: 1.3 −43 ). The structural prediction of ORF31 using PHYRE2 (99.8% confidence), SWISS-MODEL (QMEAN: −3.59) and HHPRED (98.13%, e-value: 2.1e −8 ) indicated homology to the tail fiber from bacteriophage Mu G+ (gpS), more specific the distal C-terminus (amino acids 351-449) responsible for receptor binding [37]. As evident from the superimposed alignment in Figure 3, the PHYRE2 structure prediction of ORF31 aligned exquisitely with the structure of gpS, even though they only share 21.55% identity at the amino acid level.…”

Section: Product Functional Category Clustermentioning

confidence: 99%

Phage S144, a New Polyvalent Phage Infecting Salmonella spp. and Cronobacter sakazakii

Gambino

Sørensen

Ahern

et al. 2020

IJMS

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Phages are generally considered species- or even strain-specific, yet polyvalent phages are able to infect bacteria from different genera. Here, we characterize the novel polyvalent phage S144, a member of the Loughboroughvirus genus. By screening 211 Enterobacteriaceae strains, we found that phage S144 forms plaques on specific serovars of Salmonella enterica subsp. enterica and on Cronobacter sakazakii. Analysis of phage resistant mutants suggests that the O-antigen of lipopolysaccharide is the phage receptor in both bacterial genera. The S144 genome consists of 53,628 bp and encodes 80 open reading frames (ORFs), but no tRNA genes. In total, 32 ORFs coding for structural proteins were confirmed by ESI-MS/MS analysis, whereas 45 gene products were functionally annotated within DNA metabolism, packaging, nucleotide biosynthesis and phage morphogenesis. Transmission electron microscopy showed that phage S144 is a myovirus, with a prolate head and short tail fibers. The putative S144 tail fiber structure is, overall, similar to the tail fiber of phage Mu and the C-terminus shows amino acid similarity to tail fibers of otherwise unrelated phages infecting Cronobacter. Since all phages in the Loughboroughvirus genus encode tail fibers similar to S144, we suggest that phages in this genus infect Cronobacter sakazakii and are polyvalent.

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“…Such changes lead to altered structural gene products, for example, encoding phage tail fiber assembly proteins that are necessary for phage attachment to the host cell or adsorption, which is the first step of phage infection. Adaptation can also reduce lysis time and increase phage burst size [ 43 , 44 ]. Recent findings on the Eliava Staphylococcal Bacteriophage cocktail that consist of Twort-like phage Sb-1 showed that after the adaptation process, newly formed phage clones were found in phage stock, and such a process increased phage lytic activity from 87% to 96% on globally diverse S. aureus strains.…”

Section: Discussionmentioning

confidence: 99%

S. aureus Colonization, Biofilm Production, and Phage Susceptibility in Peritoneal Dialysis Patients

et al. 2020

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Peritonitis caused by Staphylococcusaureus is of major importance in peritoneal dialysis (PD) patients due to its great virulence profile and biofilm formation ability. Bacteriophages are a potential tool to treat peritonitis resulting from biofilm-associated infections. We screened S. aureus colonization in 71 PD patients from the nasal cavity, groin, and PD exit-site regions and analyzed clinical outcomes in these patients. We performed biofilm-formation testing of different strains and compared the isolates of one patient to detect phenotypic differences in S. aureus. Phage cocktails were used to detect S. aureus in vitro susceptibility. An adaptation procedure was performed in cases of bacterial resistance. Around 30% of PD patients (n = 21) were found to be S. aureus carriers; from these, a total of 34 S. aureus strains were isolated, of which 61.8% (n = 21) produced a strong biofilm. Phenotypic differences in strain biofilm production were detected in eight patients out of ten. All strains were sensitive to commonly used antibiotics. Broadly positive phage lytic activity (100%) was observed in six cocktails out of seven, and bacterial resistance towards phages was overcome using adaptation. Overall phages showed a promising in vitro effect in biofilm-forming S. aureus strains.

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Phage tail fibre assembly proteins employ a modular structure to drive the correct folding of diverse fibres

Cited by 59 publications

References 45 publications

Isolation of Four Lytic Phages Infecting Klebsiella pneumoniae K22 Clinical Isolates from Spain

Isolation of Four Lytic Phages Infecting Klebsiella pneumoniae K22 Clinical Isolates from Spain

Phage S144, a New Polyvalent Phage Infecting Salmonella spp. and Cronobacter sakazakii

S. aureus Colonization, Biofilm Production, and Phage Susceptibility in Peritoneal Dialysis Patients

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