Phage Therapy’s Role in Combating Antibiotic-Resistant Pathogens

Lyon, Jeff

doi:10.1001/jama.2017.12938

Cited by 29 publications

(20 citation statements)

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“…The need for more targeted therapy of ALD coincides with the marked progress in research on immunobiology of bacterial viruses (phages). In the face of increasing drama of anti-microbial resistance, phages offer a broad medical promise as a weapon against antibiotics-resistant bacteria [10]. However, phages should no longer be considered as mere bacterial viruses but also as potential modulators of immunity [11][12][13][14][15][16], a finding which may have an important application in therapy including autoimmune diseases [12].…”

Section: Introductionmentioning

confidence: 99%

Therapeutic potential of phages in autoimmune liver diseases

Górski

Jończyk‐Matysiak

Łusiak-Szelachowska

et al. 2018

Clinical and Experimental Immunology

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SummaryAutoimmune liver disease (ALD) poses a difficult medical challenge, as there is a significant number of patients in whom current therapy offers questionable or no benefit, yet its side effects may be serious, including the development of malignancy. Bacterial viruses (phages) have been recognized increasingly as immunomodulators contributing to immune homeostasis and curbing inflammation. Accumulating data suggest that phages may be useful in immunotherapy of ALD. Phages have been shown to down‐regulate the expression and/or production and activity of factors associated with hepatic injury [reactive oxygen species, Toll‐like receptor (TLR)‐4 activation, nuclear factor kappa B (NF‐κB) activation, proinflammatory and procoagulant activities of platelets] and up‐regulate the expression and/or production of factors demonstrated as playing a protective role [interleukin (IL)‐10, IL‐1 receptor antagonist].

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Section: Introductionmentioning

confidence: 99%

Therapeutic potential of phages in autoimmune liver diseases

Górski

Jończyk‐Matysiak

Łusiak-Szelachowska

et al. 2018

Clinical and Experimental Immunology

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“…Amino acids with hydrophobic side chains also show positive correlation with the hydrophobicity index. As could be seen in Table 8, there were three important amino acids found in the ten top-ranked highest propensity scorers and hydrophobicity index, i.e., Ala (1, 5), Val (3,4), and Gly (4, 1). Previously, various experimental studies have mentioned that amino acid substitution could affect the stability of procapsids and virions of bacteriophages.…”

Section: Analysis Of Pvps Using Informative Physicochemical Propertiesmentioning

confidence: 99%

“…As noticed in Table 8, there were two amino acids, i.e., Gly and Thr, found in the five top-ranked amino acids having the highest propensity scores as well as alpha-helix propensity. The ranks of propensity scorers (PS, alpha-helix) for Gly and Thr were (4,1) and (2,3), respectively. Although, the rank of Ala was (1, 12), Pace et al found that the estimated differences in free energy or ∆(∆G) values in kcal/mol of Ala has been set to zero because it is usually the amino acid with the most favorable helix propensity [46].…”

Section: Analysis Of Pvps Using Informative Physicochemical Propertiesmentioning

confidence: 99%

“…It could be noticed that the property of side-chain [47] of amino acid had a high negative correlation (R = −0.516), indicating that PVPs favor small amino acids. As seen in Table 8, five top-ranked important amino acids present in PVPs were Ala, Thr, Val, Gly, and Ser, while according to the property of side-chain [50], such five amino acids (PS, side-chain) were ranked at (1,19), (2,15), (3,16), (4,20), and (5,18), respectively. Our analysis result was well consistent with the work of a previous study [45].…”

Section: Analysis Of Pvps Using Informative Physicochemical Propertiesmentioning

confidence: 99%

“…Bacteriophages irreversibly attach themselves to the surface of a susceptible host, insert their genetic information and persist using two possible strategies: lytic or lysogenic life cycle [2]. Due to their characteristics, lack of toxicity for human cells, harmless to non-specific microbes (e.g., normal flora), and their potential against antibiotic-resistant bacteria, bacteriophages are promising as alternatives to antibiotics [3]. Phage structural (virion) proteins (PVPs) including capsid proteins (coat proteins), tail proteins and phage particle enzymes, play important roles in the interaction between themselves and its host bacteria, which can be used to develop a new class of antimicrobial agents [4].…”

Section: Introductionmentioning

confidence: 99%

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PVPred-SCM: Improved Prediction and Analysis of Phage Virion Proteins Using a Scoring Card Method

Charoenkwan

Kanthawong

Schaduangrat

et al. 2020

Cells

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Although, existing methods have been successful in predicting phage (or bacteriophage) virion proteins (PVPs) using various types of protein features and complex classifiers, such as support vector machine and naïve Bayes, these two methods do not allow interpretability. However, the characterization and analysis of PVPs might be of great significance to understanding the molecular mechanisms of bacteriophage genetics and the development of antibacterial drugs. Hence, we herein proposed a novel method (PVPred-SCM) based on the scoring card method (SCM) in conjunction with dipeptide composition to identify and characterize PVPs. In PVPred-SCM, the propensity scores of 400 dipeptides were calculated using the statistical discrimination approach. Rigorous independent validation test showed that PVPred-SCM utilizing only dipeptide composition yielded an accuracy of 77.56%, indicating that PVPred-SCM performed well relative to the state-of-the-art method utilizing a number of protein features. Furthermore, the propensity scores of dipeptides were used to provide insights into the biochemical and biophysical properties of PVPs. Upon comparison, it was found that PVPred-SCM was superior to the existing methods considering its simplicity, interpretability, and implementation. Finally, in an effort to facilitate high-throughput prediction of PVPs, we provided a user-friendly web-server for identifying the likelihood of whether or not these sequences are PVPs. It is anticipated that PVPred-SCM will become a useful tool or at least a complementary existing method for predicting and analyzing PVPs.

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Magnetic Bacteriophage‐Engineered Janus Micromotors for Selective Bacteria Capture and Detection

Cuntín‐Abal,

Bujalance‐Fernández,

Yuan

et al. 2023

Adv Funct Materials

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Herein, T4‐bacteriophage‐funcionalizated magnetic Janus micromotors are used for the first time for highly selective Escherichia coli (E. coli) recognition and detection. The micromotors propel at speeds of up to 40 µm s−1 in complex biological samples for on‐the‐fly capture of E. coli “strain B”‐thiolated T4 bacteriophage complex. Detection is achieved by a simplified colorimetric readout in connection with gold nanoparticles. The detection limit meets the cut‐off for the fast diagnosis of urinary tract infections. The bacteriophage‐engineered micromotors are tested on bacteria isolated from urine samples and in serum isolated from negative blood cultures from hospital patients with excellent selectivity and reliability. The new strategy described here holds considerable promise for the multiplexed detection of a myriad of bacteria strains using tailored bacteriophages. Technically, this is the first microplate‐reader integrated micromotor approach, crossing another bridge from the research lab to the practical use of micromotors. Specifically, these results represent a qualitative step forward in the use of micromotor technology with sophisticated functionalization for fast bacteria screening in clinical settings.

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Phage Therapy’s Role in Combating Antibiotic-Resistant Pathogens

Abstract: Functional Brain Imaging Updates on the HorizonLaboratory-on-a-Chip Uses Acoustics to Isolate Tell-Tale Particles in Blood News From the CDC.

Cited by 29 publications

References 0 publications

Therapeutic potential of phages in autoimmune liver diseases

Therapeutic potential of phages in autoimmune liver diseases

PVPred-SCM: Improved Prediction and Analysis of Phage Virion Proteins Using a Scoring Card Method

Magnetic Bacteriophage‐Engineered Janus Micromotors for Selective Bacteria Capture and Detection

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