Phage types, antibiotic susceptibilities and plasmid profiles of Salmonella typhimurium and Salmonella enteritidis strains isolated in Istanbul, Turkey

Anğ-Küçüker, Mine; Tolün, Venus; Helmuth, Reiner; Rabsch, Wolfgang; Büyükbaba-Boral, Özden; Törümküney-Akbulut, D.; Susever, Serdar; Anǧ, Özdem

doi:10.1046/j.1469-0691.2000.00132.x

Cited by 15 publications

(5 citation statements)

References 42 publications

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“…Nevertheless, in our study, the same plasmid profile was related to different antimicrobial resistance profiles and vice versa. However, the mobile character and appearance of a single plasmid in different strains, such as reported in the current study, might represent a limitation to the use of plasmid analysis in epidemiological investigations [17][18][19][20][21][22][23][24][25][26][27][28][29][30]. Our results, however, showed that there is no correlation between plasmid profiles and susceptibility data.…”

Section: Discussionmentioning

confidence: 66%

“…Although phage typing has been used primarily for the epidemiological study of Salmonella, it is of little use in places where a small number of phage types predominates. Therefore, investigation of the antimicrobial susceptibilities, the characteristics of resistant strains, and the molecular epidemiology of the strains is more significant [27].…”

Section: Discussionmentioning

confidence: 99%

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Characterization of Salmonella Enteritidis isolated from foods and patients in northern Morocco

Ammari

Laglaoui²,

En-nanei

et al. 2009

J Infect Dev Ctries

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Objectives: This study aimed to investigate Salmonella Enteritidis strains isolated from human and food sources in the north of Morocco by means of phenotypic and genotypic methods. Methodology: Fifteen isolates from humans and food were submitted to phage typing, XbaI-macrorestriction (pulsed field gel electrophoresis [PFGE]), enterobacterial repetitive intergenic consensus (ERIC-PCR), antimicrobial susceptibility testing, and PCR assay targeting the spvR and invA genes. Results: Six fingerprinting profiles were obtained with the ERIC-PCR method, four with PFGE profiling, five with antimicrobial resistance, three with phage typing, and only one with plasmid profiling. spvR gene was detected in six strains, which did not harbour plasmids of 90 kb. Conclusions: The conclusions of this study are drawn from a limited number of isolates. It would be desirable to investigate a greater and more diverse population of Salmonella isolates. S. Enteritidis was genotyped and showed four different patterns by PFGE and six by ERIC-PCR. Accordingly, high genetic similarity and limited genetic diversity were found for these strains from north of Morocco.

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Section: Discussionmentioning

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Characterization of Salmonella Enteritidis isolated from foods and patients in northern Morocco

Ammari

Laglaoui²,

En-nanei

et al. 2009

J Infect Dev Ctries

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“…The S . Typhimurium PFGE subtype T1.1 from Farm One (21) was identified as phage type (PT) 193, a PT commonly associated with illnesses in humans (29–39). This Salmonella PT has also been isolated from cattle (38, 40, 41), poultry (31, 42), pigs (31, 40), and dogs (40).…”

Section: Discussionmentioning

confidence: 99%

Diversity of Antimicrobial Resistance Phenotypes in Salmonella Isolated from Commercial Poultry Farms

Liljebjelke¹,

Hofacre²,

White

et al. 2017

Front. Vet. Sci.

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Salmonella remains the leading cause of foodborne illness in the United States, and the dissemination of drug-resistant Salmonellae through the food chain has important implications for treatment failure of salmonellosis. We investigated the ecology of Salmonella in integrated broiler production in order to understand the flow of antibiotic susceptible and resistant strains within this system. Data were analyzed from a retrospective study focused on antimicrobial resistant Salmonella recovered from commercial broiler chicken farms conducted during the initial years of the US FDA’s foray into retail meat surveillance by the National Antimicrobial Resistance Monitoring System (NARMS). Sixty-three percentage of Salmonella were pan-susceptible to a panel of 19 antimicrobials used by the NARMS program. Twenty-five antimicrobial resistance phenotypes were observed in Salmonella isolated from two broiler chicken farms. However, Salmonella displaying resistance to streptomycin, alone, and in combination with other antibiotics was the most prevalent (36.3%) antimicrobial resistance phenotype observed. Resistance to streptomycin and sulfadimethoxine appeared to be linked to the transposon, Tn21. Combinations of resistance against streptomycin, gentamicin, sulfadimethoxine, trimethoprim, and tetracycline were observed for a variety of Salmonella enterica serovars and genetic types as defined by pulsed-field gel electrophoresis. There were within and between farm differences in the antibiotic susceptibilities of Salmonella and some of these differences were linked to specific serovars. However, farm differences were not linked to antibiotic usage. Analysis of the temporal and spatial distribution of the endemic Salmonella serovars on these farms suggests that preventing vertical transmission of antibiotic-resistant Salmonella would reduce carcass contamination with antibiotic-resistant Salmonella and subsequently human risk exposure.

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“…Most SE isolates harbour serovar-associated factors as the virulence, the fibrial and the antibiotic resistance genes (Lu et al, 1999;Anğu-Küçüker et al, 2000;Bakshi et al, 2003). Virulence genes encode products that assist organisms to express the virulence in the host cells.…”

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confidence: 99%

Antibiotic resistance and distribution of SodCI, sopE, sefA genes among Salmonella enteric serotype Enteritidis isolates from poultry

Ekateriniadou

Boukouvala

Papi

et al. 2018

J Hellenic Vet Med Soc

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resistance and distribution of SodCI, sopE, sefA genes among Salmonella enteric serotype Enteritidis isolates from poultry. Journal of the Hellenic Veterinary Medical Society, 66(2),[70][71][72][73][74][75][76][77][78][79] ABSTRACT. Present work aimed to examine the antibiotic resistance of the Salmonella enterica serotype Enteritidis (SE) isolates from poultry, to study the plasmid-mediated ampicillin resistance and to detect and determine the distribution of sodCI, sopE and sefA genes. Thirty-five SE isolates from one-day chicks, layers and broilers were studied for susceptibility/resistance to sixteen antimicrobial agents; 23 (65.7%) of them showed resistance to ampicillin, 5 (14.3%) to ampicillin and tetracycline, 4 (11.45%) to tetracycline and 1 (2.9%) isolate showed multi-drug resistance. Ampicillin (Amp R ) and ampicillin/tetracycline (Amp R Te R ) resistance was easily transferred by conjugation, and all isolates except two possessed a common band. The molecular mass of the plasmid carrying ampicillin resistance was approximately determined at 41kb after DNA digestion with BamHI, HindIII, EcoRI, EcoRV and PstI restriction enzymes and ligation of EcoRI fragments to pET29c. For the detection of TEM-1 or/and TEM-2 ß-lactamases, two pairs of primers were used in a polymerase chain reaction (PCR). The PCR products showed the presence of bla TEM-1 gene in all isolates. The presence of SodCI, sopE, sefA genes was also examined by PCR. Twenty-two (62,8%) isolates carried the sodCI gene, thirty-four (97,2%) isolates carried the sopE gene and all isolates carried the sefA fimbrial locus. Keywords

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Phage types, antibiotic susceptibilities and plasmid profiles of Salmonella typhimurium and Salmonella enteritidis strains isolated in Istanbul, Turkey

Abstract: The results suggest that the majority of S. typhimurium strains were closely related.

Cited by 15 publications

References 42 publications

Characterization of Salmonella Enteritidis isolated from foods and patients in northern Morocco

Characterization of Salmonella Enteritidis isolated from foods and patients in northern Morocco

Diversity of Antimicrobial Resistance Phenotypes in Salmonella Isolated from Commercial Poultry Farms

Antibiotic resistance and distribution of SodCI, sopE, sefA genes among Salmonella enteric serotype Enteritidis isolates from poultry

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