Phages and phage therapy: past, present and future

Essa, Nour; Rossitto, Martina; Fiscarelli, Ersilia

doi:10.4081/mm.2020.8709

Cited by 6 publications

(2 citation statements)

References 18 publications

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“…The lytic cycle is carried out by lytic phages, which are also known as virulent phages. During this cycle, the virus adheres to the surface of the target bacteria, injects its genome into the host cytoplasm, and stimulates the bacterial molecular system to produce virions that will eventually destroy the bacterial cell and release themselves into the environment (Essa et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

WITHDRAWN: SYNERGISM OF BACTERIOPHAGE AND ANTIBIOTICS IN THE REDUCTION OFPseudomonas aeruginosaBIOFILM

Timilsina,

Karna,

Napit

et al. 2024

Preprint

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IntroductionPseudomonas aeruginosa is resistant to most of the antibiotics. This makes treatment of Pseudomonas aeruginosa difficult. The problem is further compounded by its ability to form biofilm. The aim of this study was to isolate lytic phage against antibiotic resistant Pseudomonas aeruginosa and to use it in the reduction of Pseudomonas aeruginosa biofilm.MethodologyBacteriophage isolation was done by Double Layer Agar Assay method. Phage cocktail was used to disrupt biofilm. The synergistic effect of phage and antibiotic in reducing biofilm was analyzed. Effect of various external factors in phage stability was examined. Whole genome sequencing of phage DNA was done.ResultLytic bacteriophage against Pseudomonas aeruginosa was isolated. Three distinct bands of phage proteins of size 35, 40 and 100KDa were observed after performing SDS PAGE. Phage DNA size was determined to be larger than 10 Kb from agarose gel electrophoresis. Whole genome sequencing of phage revealed its size to be 43,428 nucleotides (43 Kb) in length and the GC content of 62.16%. There was no presence of virulence gene in the phage genome and it contained holin and endolysin gene.Calcium ion increased the phage adsorption. Phage showed stability against SDS and osmotic shock whereas it was susceptible to ethanol, acetone and CTAB. Phage TU_pse1B reduced the biofilm by 60.99% whereas Phage TU_pse1Bi reduced the biofilm by 60.37%. Synergism of phage and antibiotic was observed in reducing biofilm. Phage plus antibiotic reduced biofilm by further 26.67% than phage alone.ConclusionPhage TU_pse1B showed good stability to various physiochemical factors as well as it was efficient in reducing Pseudomonas aeruginosa biofilm. So, this phage can be a good candidate for controlling antibiotic resistant Pseudomonas aeruginosa.

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Section: Introductionmentioning

confidence: 99%

WITHDRAWN: SYNERGISM OF BACTERIOPHAGE AND ANTIBIOTICS IN THE REDUCTION OFPseudomonas aeruginosaBIOFILM

Timilsina,

Karna,

Napit

et al. 2024

Preprint

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“…Additionally, bacteriophages are present in the surrounding environment of soil, air, and water and infect only target bacteria without any negative effects on humans or plants. Accordingly, phage therapy is non-toxic, eliminating just target bacteria, and requires small doses ( Essa et al, 2020 ; Loc-Carrillo and Abedon, 2011 ; Principi et al, 2019 ).…”

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confidence: 99%

Isolation of Nine Bacteriophages Shown Effective against Erwinia amylovora in Korea

et al. 2022

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Erwinia amylovora is a devastating bacterial plant pathogen that infects Rosaceae including apple and pear and causes fire blight. Bacteriophages have been considered as a biological control agent for preventing bacterial infections of plants. In this study, nine bacteriophages (ΦFifi011, ΦFifi044, ΦFifi051, ΦFifi067, ΦFifi106, ΦFifi287, ΦFifi318, ΦFifi450, and ΦFifi451) were isolated from soil and water samples in seven orchards with fire blight in Korea. The genetic diversity of bacteriophage isolates was confirmed through restriction fragment length polymorphism pattern analysis. Host range of the nine phages was tested against 45 E. amylovora strains and 14 E. pyrifoliae strains and nine other bacterial strains. Among the nine phages, ΦFifi044 and ΦFifi451 infected and lysed E. amylovora only. And the remaining seven phages infected both E. amylovora and E. pyrifoliae. The results suggest that the isolated phages were different from each other and effective to control E. amylovora, providing a basis to develop biological agents and utilizing phage cocktails.

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Progress of phage therapy research as an alternative to antibiotics: Current status, challenges, and the future of phage therapeutics.

Ogungbe,

Awoniyi,

Bolarinde

et al. 2024

Journal of Medicine, Surgery, and Public Health

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Phages and phage therapy: past, present and future

Cited by 6 publications

References 18 publications

WITHDRAWN: SYNERGISM OF BACTERIOPHAGE AND ANTIBIOTICS IN THE REDUCTION OFPseudomonas aeruginosaBIOFILM

WITHDRAWN: SYNERGISM OF BACTERIOPHAGE AND ANTIBIOTICS IN THE REDUCTION OFPseudomonas aeruginosaBIOFILM

Isolation of Nine Bacteriophages Shown Effective against Erwinia amylovora in Korea

Progress of phage therapy research as an alternative to antibiotics: Current status, challenges, and the future of phage therapeutics.

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