Phages in Food Industry Biocontrol and Bioremediation

Cristobal-Cueto, Pablo; García-Quintanilla, Alberto; Esteban, Jaime; García-Quintanilla, Meritxell

doi:10.3390/antibiotics10070786

Cited by 43 publications

(33 citation statements)

References 99 publications

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“…Likewise, Bardina et al [69] administered a solution of phage cocktail against S. Typhimurium twice a day on days four and five after infection, which reduced the bacterium by one logarithmic unit on days five and six after treatment. This reduction in bacterial loads and shedding of phages could also have further benefits, as it could actively lower Salmonella loads in the environment, reducing the potential for feedback contamination [70,71].…”

Section: Elimination Of Se Pt13a In Chickensmentioning

confidence: 99%

Efficacy of Salmonella Bacteriophage S1 Delivered and Released by Alginate Beads in a Chicken Model of Infection

Gómez-García

Chávez-Carbajal

Segundo-Arizmendi

et al. 2021

Viruses

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Modern bacteriophage encapsulation methods based on polymers such as alginate have been developed recently for their use in phage therapy for veterinary purposes. In birds, it has been proven that using this delivery system allows the release of the bacteriophage in the small intestine, the site of infection by Salmonella spp. This work designed an approach for phage therapy using encapsulation by ionotropic gelation of the lytic bacteriophage S1 for Salmonella enterica in 2% w/v alginate beads using 2% w/v calcium chloride as crosslinking agent. This formulation resulted in beads with an average size of 3.73 ± 0.04 mm and an encapsulation efficiency of 70%. In vitro, the beads protected the bacteriophages from pH 3 and released them at higher pH. To confirm that this would protect the bacteriophages from gastrointestinal pH changes, we tested the phage infectivity in vivo assay. Using a model chicken (Gallus gallus domesticus) infected with Salmonella Enteritidis, we confirmed that after 3 h of the beads delivery, infective phages were present in the chicken’s duodenal and caecal sections. This study demonstrates that our phage formulation is an effective system for release and delivery of bacteriophage S1 against Salmonella Enteritidis with potential use in the poultry sector.

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Section: Elimination Of Se Pt13a In Chickensmentioning

confidence: 99%

Efficacy of Salmonella Bacteriophage S1 Delivered and Released by Alginate Beads in a Chicken Model of Infection

Gómez-García

Chávez-Carbajal

Segundo-Arizmendi

et al. 2021

Viruses

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“…As a further potential improvement, at the moment limited to the hospital environment, specific removal of individual bacterial targets could be accomplished by the addition of lytic bacteriophages to cleansers. Bacteriophages are viruses infecting only bacteria with high specificity and rapidity, and their successful applicability has been reported for the decontamination of food and food-processing surfaces in industrial settings [111]. No studies on the effectiveness of phages in domestic environments are available yet, however their effective application against MDR bacterial strains was reported in the hospital environment [112,113], suggesting that a combined phage and probiotic-based cleaning may provide an effective way to stabilize the BE microbiome in different settings.…”

Section: A New Paradigm Of the Environmental Health: Focus On Microbi...mentioning

confidence: 99%

Pathogen Control in the Built Environment: A Probiotic-Based System as a Remedy for the Spread of Antibiotic Resistance

et al. 2022

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The high and sometimes inappropriate use of disinfectants and antibiotics has led to alarming levels of Antimicrobial Resistance (AMR) and to high water and hearth pollution, which today represent major threats for public health. Furthermore, the current SARS-CoV-2 pandemic has deeply influenced our sanitization habits, imposing the massive use of chemical disinfectants potentially exacerbating both concerns. Moreover, super-sanitation can profoundly influence the environmental microbiome, potentially resulting counterproductive when trying to stably eliminate pathogens. Instead, environmentally friendly procedures based on microbiome balance principles, similar to what applied to living organisms, may be more effective, and probiotic-based eco-friendly sanitation has been consistently reported to provide stable reduction of both pathogens and AMR in treated-environments, compared to chemical disinfectants. Here, we summarize the results of the studies performed in healthcare settings, suggesting that such an approach may be applied successfully also to non-healthcare environments, including the domestic ones, based on its effectiveness, safety, and negligible environmental impact.

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“…Similarly, a cocktail of phages is widely used to control antibiotic-resistant Staphylococcus aureus and P. aeruginosa in the treatment of chronic otitis infections [64]. In the food industry, phage cocktails are used as an innovative therapeutic approach to treat various foodborne pathogens, such as Salmonella entrica, Listeria monocytogenes, Escherichia coli, Shigellasonnei, and Campylobacter jejuni for pre-and post-harvesting food protection and processing to save millions of lives from food poison-mediated diseases [71][72][73][74]. P1 cocktail consisting of six phage isolations may kill 98% of R. solanacearum through direct application via soil drenching for the biocontrol of bacterial wilt disease of potato and tomato [61,75].…”

Section: Phage Cocktails As Antibacterial Therapeutic Agentsmentioning

confidence: 99%

Deploying Viruses against Phytobacteria: Potential Use of Phage Cocktails as a Multifaceted Approach to Combat Resistant Bacterial Plant Pathogens

Farooq

Hussain

Shakeel

et al. 2022

Viruses

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Plants in nature are under the persistent intimidation of severe microbial diseases, threatening a sustainable food production system. Plant-bacterial pathogens are a major concern in the contemporary era, resulting in reduced plant growth and productivity. Plant antibiotics and chemical-based bactericides have been extensively used to evade plant bacterial diseases. To counteract this pressure, bacteria have evolved an array of resistance mechanisms, including innate and adaptive immune systems. The emergence of resistant bacteria and detrimental consequences of antimicrobial compounds on the environment and human health, accentuates the development of an alternative disease evacuation strategy. The phage cocktail therapy is a multidimensional approach effectively employed for the biocontrol of diverse resistant bacterial infections without affecting the fauna and flora. Phages engage a diverse set of counter defense strategies to undermine wide-ranging anti-phage defense mechanisms of bacterial pathogens. Microbial ecology, evolution, and dynamics of the interactions between phage and plant-bacterial pathogens lead to the engineering of robust phage cocktail therapeutics for the mitigation of devastating phytobacterial diseases. In this review, we highlight the concrete and fundamental determinants in the development and application of phage cocktails and their underlying mechanism, combating resistant plant-bacterial pathogens. Additionally, we provide recent advances in the use of phage cocktail therapy against phytobacteria for the biocontrol of devastating plant diseases.

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Phages in Food Industry Biocontrol and Bioremediation

Cited by 43 publications

References 99 publications

Efficacy of Salmonella Bacteriophage S1 Delivered and Released by Alginate Beads in a Chicken Model of Infection

Efficacy of Salmonella Bacteriophage S1 Delivered and Released by Alginate Beads in a Chicken Model of Infection

Pathogen Control in the Built Environment: A Probiotic-Based System as a Remedy for the Spread of Antibiotic Resistance

Deploying Viruses against Phytobacteria: Potential Use of Phage Cocktails as a Multifaceted Approach to Combat Resistant Bacterial Plant Pathogens

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