Phages of Pseudomonas

Hayashi, Tetsuya; Nakayama, Keisuke

doi:10.1007/978-1-4419-9086-0_8

Cited by 1 publication

(1 citation statement)

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“…During the course of our efforts to edit the extant genome of the soil bacterium Pseudomonas putida KT2440 as an optimal chassis for industrial and environmental biotechnology (Nikel et al ., ), we faced the dilemma of either keeping or deleting the six regions that are annotated in the ACLAME database of mobile prokaryotic DNA (http://aclame.ulb.ac.be; Leplae et al ., ) as bona fide proviral sequences. These six regions seem to actually encode four prophages, as two of them lay adjacent to the others in contiguous operons (Canchaya et al ., ; Hayashi and Nakayama, ; Wu et al ., ). Taken together, these prophage regions comprise approximately 2.6% of the genome.…”

Section: Introductionmentioning

confidence: 98%

Freeing Pseudomonas putida KT2440 of its proviral load strengthens endurance to environmental stresses

Martínez‐García

Jatsenko

Kivisaar

et al. 2014

Environmental Microbiology

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2.6% of the genome of the soil bacterium Pseudomonas putida KT2440 encodes phage-related functions, but the burden of such opportunistic DNA on the host physiology is unknown. Each of the four apparently complete prophages borne by this strain was tested for stability, spontaneous excision and ability to cause lysis under various stressing conditions. While prophages P3 (PP2266-PP2297) and P4 (PP1532-1584) were discharged from the genome at a detectable rate, their induction failed otherwise to yield infective viruses. Isogenic P. putida KT2440 derivatives bearing single and multiple deletions of each of the prophages were then subjected to thorough phenotypic analyses, which generally associated the loss of proviral DNA with an increase of physiological vigour. The most conspicuous benefit acquired by prophage-less cells was a remarkable improvement in tolerance to UV light and other insults to DNA. This was not accompanied, however, with an upgrade of recA-mediated homologous recombination. The range of tolerance to DNA damage gained by the prophage-free strain was equivalent to the UV resistance endowed by the TOL plasmid pWW0 to the wild-type bacterium. While the P. putida's prophages are therefore genuinely parasitic, their detrimental effects can be offset by acquisition of compensatory traits through horizontal gene transfer.

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