Phagocytic activity and oxygen metabolism of peripheral blood granulocytes from rabbits experimentally infected with <i>Trichophyton mentagrophytes</i>

Wojcicka-Lorenowicz, K.; Kostro, K.; Lisiecka, Urszula; Gąsiorek, Bolesław

doi:10.2478/jvetres-2018-0006

Cited by 5 publications

(3 citation statements)

References 15 publications

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“…The phagocytic and oxidative burst activities of peripheral blood granulocytes and monocytes were quantified using commercially available kits as described previously 17 , 18 and analyzed using the Epics XL flow cytometer (Beckman Coulter, Florida).…”

Section: Methodsmentioning

confidence: 99%

The effect of pegbovigrastim administration on the nonspecific immunity of calves

Dudek,

Szacawa,

Bednarek

2023

Veterinary Internal Medicne

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BackgroundPrevention of diseases in the early rearing of calves is important, particularly because disease occurrence most often requires antimicrobial administration but reduction of their use in animals is a priority. Pegbovigrastim is known for its use as an immunoregulator in cows and heifers, but the effect of its administration on calves has not been fully investigated.ObjectivesInvestigate whether administration of pegbovigrastim effectively stimulates nonspecific immunity in healthy calves.AnimalsEleven clinically healthy 5‐week‐old calves.MethodsProspective observational study. Calves were randomly allocated to an experimental or control groups to receive pegbovigrastim or the same volume of phosphate‐buffered saline twice over a 7‐day period. To evaluate nonspecific immunity, the numbers of total leukocytes and cells in the appropriate cell fractions were determined. Cytometric analyses were carried out to identify cells expressing CD11b and to evaluate the phagocytic and oxidative burst activities of granulocytes and monocytes. Myeloperoxidase (MPO) and selected cytokines were assayed using ELISA.ResultsPegbovigrastim significantly increased the number of total leukocytes and of cells in all of the examined subsets (P < .05). The phagocytic activity of leukocytes expressed as mean fluorescence intensity was significantly potentiated after pegbovigrastim administration (P < .05). The cytokine response was modulated by pegbovigrastim administration toward anti‐inflammatory activity.Conclusions and Clinical ImportancePegbovigrastim effectively stimulated nonspecific immunity in clinically healthy calves, which in the long term could make the prevention of diseases during early rearing possible by strengthening the immune defense mechanisms of the host.

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Section: Methodsmentioning

confidence: 99%

The effect of pegbovigrastim administration on the nonspecific immunity of calves

Dudek,

Szacawa,

Bednarek

2023

Veterinary Internal Medicne

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“…The phagocytic activity of the granulocytes and monocytes was determined in heparinised whole blood samples using a commercial Phagotest kit (Glycotope Biotechnology, Heidelberg, Germany) according to Wojcicka-Lorenowicz et al . ( 41 ) with modifications. The percentages of granulocytes and monocytes that had phagocytic activity against bacteria was evaluated, and the mean fluorescence intensity (MFI) of the phagocytising cell population was assessed to estimate the individual cellular phagocytic activity, i.e .…”

Section: Methodsmentioning

confidence: 99%

“…The oxidative burst activity of the granulocytes and monocytes was examined in heparinised whole blood samples using a commercial Phagoburst kit (Glycotope Biotechnology, Heildelberg, Germany) according to Wojcicka-Lorenowicz et al . ( 41 ) with modifications. The samples were analysed by flow cytometry using the same device as previously described within 30 min.…”

Section: Methodsmentioning

confidence: 99%

A pilot study on the effect of a novel feed additive containing exogenous enzymes, acidifiers, sodium butyrate and silicon dioxide nanoparticles on selected cellular immune indices and body weight gains of calves

Szacawa

Dudek

Bednarek

et al. 2021

Journal of Veterinary Research

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Introduction The rearing of calves is a difficult period for farmers due to health problems to which the animals are prone this time. Since the use of antibiotics as growth promoters has been forbidden, various innovative feed additives have been tested in many countries around the world. Material and Methods In this study, experimental (E) calves were supplemented with a novel feed additive consisting of the pancreatic-like enzymes protease and lipase, a fat-coated mixture of organic fumaric, malic, citric and sorbic acids, sodium butyrate and silicon dioxide nanoparticles. Control (C) calves received feed without additive. During the supplementation, white blood cell (WBC) counts with leukocyte differentiation, percentages of B lymphocytes and T lymphocytes and their subpopulations, phagocytic activity and oxidative burst of circulating monocytes and granulocytes were examined. Body weight (b.w.) gains of the calves were also monitored. Results The WBC counts in the E and C calves were within the reference ranges throughout the study. In the analysis of the percentages of the lymphocyte subpopulations, phagocytic activity and oxidative burst, no statistically significant differences were reported between the E and C groups. However, higher average daily body weight gains were obtained for the E calves. Conclusion The study revealed that the examined feed additive did not modulate the immune response of the calves significantly. The tendency to higher daily average b.w. gains in the E calves than in the C calves suggests a beneficial effect of this feed additive.

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The effect of Cutaneous Candidiasis infection on some physiological and immunological parameters

Al-Dhwahery

Al-Awadi

AL-Brykiat

2019

The 7th International Conference on Applied Science and Technology (Icast 2019)

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Phagocytic activity and oxygen metabolism of peripheral blood granulocytes from rabbits experimentally infected with Trichophyton mentagrophytes

Cited by 5 publications

References 15 publications

The effect of pegbovigrastim administration on the nonspecific immunity of calves

The effect of pegbovigrastim administration on the nonspecific immunity of calves

A pilot study on the effect of a novel feed additive containing exogenous enzymes, acidifiers, sodium butyrate and silicon dioxide nanoparticles on selected cellular immune indices and body weight gains of calves

The effect of Cutaneous Candidiasis infection on some physiological and immunological parameters

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