Phagocytosis and Killing of Salmonella by 7-Hydroxycoumarin Activated Macrophages

Stefanova, Tsvetanka; Nikolova, Nikolina; Neychev, H.; Zlabinger, Gerhard J.

doi:10.3109/08820139.2011.619021

Cited by 7 publications

(8 citation statements)

References 26 publications

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“…UMB also reduces the incidence of lymphoedema in humans [14] . Of note, antioxidative, anti-inflammatory and immunomodulatory effects of UMB in both, in vitro and in vivo studies have been reported elsewhere [8,[15][16][17][18][19][20][21] .…”

Section: Introductionmentioning

confidence: 99%

Assessment of the Combined Treatment with Umbelliferone and Four Classical Antiepileptic Drugs Against Maximal Electroshock-Induced Seizures in Mice

et al. 2015

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Background/Aims: The aim of this study was to determine the effects of umbelliferone (7-hydroxycoumarin; UMB) on the anticonvulsant potency of four classical antiepileptic drugs (carbamazepine (CBZ), phenytoin (PHT), phenobarbital (PB) and valproate (VPA)) in the mouse maximal electroshock-induced seizure (MES) model. Results: UMB administered systemically intraperitoneally (ip) in a dose of 150 mg/kg significantly elevated the threshold for maximal electroconvulsions (p < 0.05) in mice. Moreover, UMB (150 mg/kg) co-administered with PB and VPA significantly enhanced the anticonvulsant potency of these drugs by reducing their median effective doses (ED₅₀ values) from 35.39 to 21.78 mg/kg (p < 0.01) for PB, and from 281.4 to 215.5 mg/kg (p < 0.01) for VPA. In contrast, UMB (150 mg/kg, ip) had no significant effect on the antiseizure activity of CBZ and PHT in the mouse MES model. Neither total brain PB, nor total brain VPA concentrations were altered after ip administration of UMB, indicating a pharmacodynamic nature of interactions between the tested drugs. Conclusions: The selective potentiation of the anticonvulsant potency of PB and VPA by UMB, and lack of any pharmacokinetic interactions between drugs, make the combinations of UMB with PB or VPA worthy of consideration for epileptic patients who are refractory to standard antiepileptic treatment.

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Section: Introductionmentioning

confidence: 99%

Assessment of the Combined Treatment with Umbelliferone and Four Classical Antiepileptic Drugs Against Maximal Electroshock-Induced Seizures in Mice

et al. 2015

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“…Previously several studies showed that umbelliferone exerts anti-inflammatory, antioxidant, antidiabetic, antinociceptive, and anticancer effects [ 27 ]. Moreover, the effects of umbelliferone on bacteria have been also reported [ 28 – 30 ]. Stefanova et al in an experimental study, using Salmonella enterica serovar Typhimurium infection in mice, documented that 7-hydroxycoumarin pretreatment is able to reduce both tissue damaging and immunosuppressive effects of the oxidative stress related to the infection [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, the effects of umbelliferone on bacteria have been also reported [ 28 – 30 ]. Stefanova et al in an experimental study, using Salmonella enterica serovar Typhimurium infection in mice, documented that 7-hydroxycoumarin pretreatment is able to reduce both tissue damaging and immunosuppressive effects of the oxidative stress related to the infection [ 28 ]. Lee and coworkers showed that umbelliferone is able to inhibit biofilm E. coli O157:H7 formation by more than 80% [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

The Efficacy of Umbelliferone, Arbutin, and N-Acetylcysteine to Prevent Microbial Colonization and Biofilm Development on Urinary Catheter Surface: Results from a Preliminary Study

Cai

Gallelli

Meacci³

et al. 2016

Journal of Pathogens

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We evaluated, in a preliminary study, the efficacy of umbelliferone, arbutin, and N-acetylcysteine to inhibit biofilm formation on urinary catheter. We used 20 urinary catheters: 5 catheters were incubated with Enterococcus faecalis (control group); 5 catheters were incubated with E. faecalis in presence of umbelliferone (150 mg), arbutin (60 mg), and N-acetylcysteine (150 mg) (group 1); 5 catheters were incubated with E. faecalis in presence of umbelliferone (150 mg), arbutin (60 mg), and N-acetylcysteine (400 mg) (group 2); and 5 catheters were incubated with E. faecalis in presence of umbelliferone (300 mg), arbutin (60 mg), and N-acetylcysteine (150 mg) (group 3). After 72 hours, planktonic microbial growth and microorganisms on catheter surface were assessed. In the control group, we found a planktonic load of ≥105 CFU/mL in the inoculation medium and retrieved 3.69 × 106 CFU/cm from the sessile cells adherent to the catheter surface. A significantly lower amount in planktonic (p < 0.001) and sessile (p = 0.004) bacterial load was found in group 3, showing <100 CFU/mL and 0.12 × 106 CFU/cm in the incubation medium and on the catheter surface, respectively. In groups 1 and 2, 1.67 × 106 CFU/cm and 1.77 × 106 CFU/cm were found on catheter surface. Our results document that umbelliferone, arbutin, and N-acetylcysteine are able to reduce E. faecalis biofilm development on the surface of urinary catheters.

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“…Some members of this family exhibit cell toxicity that has been harnessed in anti-tumor applications 103-105 , while others do not. A modeling approach similar to that presented herein would shed light on the molecular mechanisms underlying differential membrane-mediated toxicity of these compounds.…”

Section: Conclusion and Summarymentioning

confidence: 99%

Structural perturbation of a dipalmitoylphosphatidylcholine (DPPC) bilayer by warfarin and its bolaamphiphilic analogue: A molecular dynamics study

Ayee

Roth

Akpa

2016

Journal of Colloid and Interface Science

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Compounds with nominally similar biological activity may exhibit differential toxicity due to differences in their interactions with cell membranes. Many pharmaceutical compounds are amphiphilic and can be taken up by phospholipid bilayers, interacting strongly with the lipid-aqueous interface whether or not subsequent permeation through the bilayer is possible. Bolaamphiphilic compounds, which possess two hydrophilic ends and a hydrophobic linker, can likewise undergo spontaneous uptake by bilayers. While membrane-spanning bolaamphiphiles can stabilize membranes, small molecules with this characteristic have the potential to create membrane defects via disruption of bilayer structure and dynamics. When compared to the amphiphilic therapeutic anticoagulant, warfarin, the bolaamphiphilic analogue, brodifacoum, exhibits heightened toxicity that goes beyond superior inhibition of the pharmacological target enzyme. We explore, herein, the consequences of anticoagulant accumulation in a dipalmitoylphosphatidylcholine (DPPC) bilayer. Coarse-grained molecular dynamics simulations reveal that permeation of phospholipid bilayers by brodifacoum causes a disruption of membrane barrier function that is driven by the bolaamphiphilic nature and size of this molecule. We find that brodifacoum partitioning into bilayers causes membrane thinning and permeabilization and promotes lipid flip-flop – phenomena that are suspected to play a role in triggering cell death. These phenomena are either absent or less pronounced in the case of the less toxic, amphiphilic compound, warfarin.

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Phagocytosis and Killing of Salmonella by 7-Hydroxycoumarin Activated Macrophages

Cited by 7 publications

References 26 publications

Assessment of the Combined Treatment with Umbelliferone and Four Classical Antiepileptic Drugs Against Maximal Electroshock-Induced Seizures in Mice

Assessment of the Combined Treatment with Umbelliferone and Four Classical Antiepileptic Drugs Against Maximal Electroshock-Induced Seizures in Mice

The Efficacy of Umbelliferone, Arbutin, and N-Acetylcysteine to Prevent Microbial Colonization and Biofilm Development on Urinary Catheter Surface: Results from a Preliminary Study

Structural perturbation of a dipalmitoylphosphatidylcholine (DPPC) bilayer by warfarin and its bolaamphiphilic analogue: A molecular dynamics study

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