Phagocytosis and Killing of Suspended and Adhered Bacteria by Peritoneal Cells after Dialysis

Calame, Wim; Afram, Charles; Blijleven, Nico; Hendrickx, Roeland J.B.M.; Namavar, F.; Beelen, Robert H.J.

doi:10.1177/089686089501500407

Cited by 5 publications

(3 citation statements)

References 20 publications

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“…Some studies have shown that peritoneal macrophages display decreased secretion of cytokines, including TNF-␣, IL-1␤, IL-6, and IL-8 (19,40), whereas others have reported no significant change in macrophage cytokine levels in response to dialysis (29). Moreover, Calame et al (6) found that phagocytosis and killing of adhered bacteria by macrophages were not affected by glucose-containing dialysate but rather were enhanced significantly by long dwell time. The reasons for dysfunction of peritoneal macrophages could relate directly to the use of bioincompatible dialysis buffer or to the specific disease causing ESKD (14,15).…”

Section: Discussionmentioning

confidence: 99%

Therapeutic potential of regulatory macrophages generated from peritoneal dialysate in adriamycin nephropathy

Cao

Wang

et al. 2018

American Journal of Physiology-Renal Physiology

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Cell therapy using macrophages requires large amounts of cells, which are difficult to collect from patients. Patients undergoing peritoneal dialysis (PD) discard huge numbers of peritoneal macrophages in dialysate daily. Macrophages can be modulated to become regulatory macrophages, which have shown great promise as a therapeutic strategy in experimental kidney disease and human kidney transplantation. This study aimed to examine the potential of using peritoneal macrophages (PMs) from peritoneal dialysate to treat kidney disease. Monocytes/macrophages accounted for >40% of total peritoneal leukocytes in both patients and mice undergoing PD. PMs from patients and mice undergoing PD were more mature than peripheral monocytes/macrophages, as shown by low expression of C-C motif chemokine receptor 2 (CCR2) and morphological changes during in vitro culture. PMs from patients and mice undergoing PD displayed normal macrophage function and could be modulated into a regulatory (M2) phenotype. In vivo, adoptive transfer of peritoneal M2 macrophages derived from PD mice effectively protected against kidney injury in mice with adriamycin nephropathy (AN). Importantly, the transfused peritoneal M2 macrophages maintained their M2 phenotype in kidney of AN mice. In conclusion, PMs derived from patients and mice undergoing PD exhibited conventional macrophage features. Peritoneal M2 macrophages derived from PD mice are able to reduce kidney injury in AN, suggesting that peritoneal macrophages from patients undergoing PD may have the potential for clinical therapeutic application.

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Section: Discussionmentioning

confidence: 99%

Therapeutic potential of regulatory macrophages generated from peritoneal dialysate in adriamycin nephropathy

Cao

Wang

et al. 2018

American Journal of Physiology-Renal Physiology

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“…After 12 h, these cultures were incubated in fresh TSB (1:100) and stirred for 2 h at 37°C. Cultures were pelleted, washed, and resuspended in phosphate-buffered saline (PBS) (10 mM, pH 7.4 at 4°C) and adjusted to 5 × 10 8 colony-forming units (CFU)/mL according to the McFarland nephelometric scale (Nefelobac, Probac Brazil, Brazil) (26). One milliliter of bacterial suspension was injected intraperitoneally (i.p.)…”

Section: Methodsmentioning

confidence: 99%

“…Blood cells were collected via the abdominal aorta. Peritoneal lavage fluid (PeLF) was collected with 10 mL of sterile PBS using a sterile syringe inserted into the peritoneum (26). Cells from the peritoneal cavity were centrifuged at 500 × g and 4°C for 10 min (5810R, Eppendorf, USA).…”

Section: Methodsmentioning

confidence: 99%

Cytokine and Adhesion Molecule Expression Induced by Different Strains of Staphylococcus aureus in Type 1 Diabetic Rats: Role of Insulin

et al. 2019

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Introduction: Staphylococcus aureus may provoke peritonitis and death, especially in immunocompromized individuals such as diabetic patients. We evaluated the role of insulin in S. aureus-induced peritoneal infection in diabetic and non-diabetic rats.Materials/Methods: Alloxan-diabetic male Wistar rats and their respective controls received intraperitoneal injections of different strains of S. aureus or sterile phosphate-buffered saline. After 3 days of infection, the first set of diabetic and non-diabetic rats received 4 and 1 IU, respectively, of neutral protamine Hagedorn insulin and were analyzed 8 h later. The second set of diabetic and non-diabetic rats received 4 and 1 IU, respectively, of insulin 2 h before intraperitoneal infection and a half dose of insulin at 5 p.m. for the next 2 days and were analyzed 16 h later. The following measurements were performed: (a) number of cells in the peritoneal lavage fluid (PeLF), white blood cell count, and blood glucose; (b) serum insulin and corticosterone; (c) cytokine levels in the PeLF; (d) expression of adhesion molecules in the vascular endothelium; and (e) microbicidal activity.Results: Diabetic rats showed an increased number of polymorphonuclear leukocytes (PMNs) and increased concentrations of CINC-1, IL-4, and IFN-γ in the PeLF after infection with the ATCC 25923 or N315 αHL+ strain. The mesenteric expression of PECAM-1 was increased after infection with the N315 HLA+ strain. ICAM-1 expression was increased with ATCC infection. Treatment of diabetic rats with a single dose of insulin restored CINC-1 levels in the PeLF for both strains; however, PMN migration, IL-4, and IFN-γ were restored in rats infected with the ATCC strain, whereas the PeLF concentrations of CINC-2, IL-1β, and IL-4 were increased in N315-infected animals. Insulin restored PMN migration and CINC-2 levels in the PeLF in ATCC-infected rats. After multiple treatments with insulin, the levels of IL-1β, IL-6, and IFN-γ were increased in the PeLF of diabetic rats after infection with either strain, and CINC-2 levels were restored in N315-infected animals.Conclusion: These results suggest that insulin distinctively modulates cytokine production or release, PMN leukocyte migration, and adhesion molecule expression during the course of peritonitis induced by different strains of S. aureus.

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Involvement of the phosphoryl transfer network on cardiac energetic metabolism during Staphylococcus aureus infection and its association to disease pathophysiology

Perin

Baldissera

Jaguezeski

et al. 2019

Microbial Pathogenesis

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Phagocytosis and Killing of Suspended and Adhered Bacteria by Peritoneal Cells after Dialysis

Cited by 5 publications

References 20 publications

Therapeutic potential of regulatory macrophages generated from peritoneal dialysate in adriamycin nephropathy

Therapeutic potential of regulatory macrophages generated from peritoneal dialysate in adriamycin nephropathy

Cytokine and Adhesion Molecule Expression Induced by Different Strains of Staphylococcus aureus in Type 1 Diabetic Rats: Role of Insulin

Involvement of the phosphoryl transfer network on cardiac energetic metabolism during Staphylococcus aureus infection and its association to disease pathophysiology

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