Phagocytosis of bacterial strains isolated from acute dentoalveolar abscess

Lewis, Michael Alexander Oxenham; Milligan, S. G.; MacFarlane, T. W.; Carmichael, Francis A.

doi:10.1099/00222615-38-2-151

Cited by 8 publications

(4 citation statements)

References 12 publications

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“…Interestingly, in cases with polymicrobial intra‐amniotic infection, cocci were the most commonly observed microorganisms engulfed by amniotic fluid neutrophils, suggesting that these innate immune cells prefer to engulf this genus. This finding is consistent with a previous report demonstrating that neutrophils favor the ingestion of Gram‐positive cocci over Gram‐negative bacilli . Yet, our ex vivo phagocytosis assays showed that amniotic fluid neutrophils can also phagocytize bacilli.…”

Section: Discussionsupporting

confidence: 93%

Amniotic fluid neutrophils can phagocytize bacteria: A mechanism for microbial killing in the amniotic cavity

Gomez‐Lopez

Romero

Garcia‐Flores

et al. 2017

American J Rep Immunol

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Problem Neutrophils are capable of performing phagocytosis, a primary mechanism for microbial killing. Intra-amniotic infection is characterized by an influx of neutrophils into the amniotic cavity. Herein, we investigated whether amniotic fluid neutrophils could phagocytize bacteria found in the amniotic cavity of women with intra-amniotic infection. Methods Amniotic fluid neutrophils from women with intra-amniotic infection were visualized by transmission electron microscopy (n=6). The phagocytic activity of amniotic fluid neutrophils from women with intra-amniotic infection and/or inflammation (n=10) or peripheral neutrophils from healthy individuals (controls, n=3) was tested using ex vivo phagocytosis assays coupled with live imaging. Phagocytosis by amniotic fluid neutrophils was also visualized by confocal microscopy (n=10) as well as scanning and transmission electron microscopy (n=5). Results 1) Intra-amniotic infection-related bacteria including cocci (e.g. Streptococcus agalactiae), bacilli (e.g. Bacteriodes fragilis and Prevotella spp.), and small bacteria without a cell wall (e.g. Ureaplasma urealyticum) were found inside of amniotic fluid neutrophils; 2) peripheral neutrophils (controls) rapidly phagocytized Streptococcus agalactiae, Ureaplasma urealyticum, Gardnerella vaginalis, and Escherichia coli; 3) amniotic fluid neutrophils can rapidly phagocytize Streptococcus agalactiae and Gardnerella vaginalis; and 4) amniotic fluid neutrophils can slowly phagocytize Ureaplasma urealyticum and Escherichia coli; yet, the process of phagocytosis of the genital mycoplasma was lengthier. Conclusions Amniotic fluid neutrophils can phagocytize bacteria found in the amniotic cavity of women with intra-amniotic infection, namely Streptococcus agalactiae, Ureaplasma urealyticum, Gardnerella vaginalis, and Escherichia coli. Yet, differences in the rapidity of phagocytosis were observed among the studied microorganisms. These findings provide a host defense mechanism whereby amniotic fluid neutrophils can kill microbes invading the amniotic cavity.

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Section: Discussionsupporting

confidence: 93%

Amniotic fluid neutrophils can phagocytize bacteria: A mechanism for microbial killing in the amniotic cavity

Gomez‐Lopez

Romero

Garcia‐Flores

et al. 2017

American J Rep Immunol

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“…Peripheral blood PMN were less able to phagocytose strains of gram-negative anaerobic rods isolated from acute dentoalveolar abscesses than strains of gram-positive cocci [12]. In our study the negative influence of a leukotoxin-producing strain of A. actinomycetemcomitans on phagocytosis was demonstrated.…”

Section: Discussioncontrasting

confidence: 48%

Phagocytosis of Periodontopathogenic Bacteria by Crevicular Granulocytes Is Depressed in Progressive Periodontitis

Eick¹,

Pfister²,

Sigusch³

et al. 2000

Infection

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“…Fn has been shown to be resistant to phagocytosis by PMN, although the mechanism was not identified. 46 Pg and Fn cocultured together or alone with mouse DCs show that Pg is the driving force for whether or not by Fn activates DCs. Although uptake was not assessed, in one study the influence of Pg on Fn was attributed to gingipains 47 In a separate study, Fn extracts have been shown to have a suppressive effect on cell-mediated immunity and phagocyte functions, although no mechanism was not identified.…”

Section: Discussionmentioning

confidence: 95%

Polymicrobial synergy within oral biofilm promotes invasion of dendritic cells and survival of consortia members

El‐Awady

Rabelo

Meghil

et al. 2019

npj Biofilms Microbiomes

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Years of human microbiome research have confirmed that microbes rarely live or function alone, favoring diverse communities. Yet most experimental host-pathogen studies employ single species models of infection. Here, the influence of three-species oral microbial consortium on growth, virulence, invasion and persistence in dendritic cells (DCs) was examined experimentally in human monocyte-derived dendritic cells (DCs) and in patients with periodontitis (PD). Cooperative biofilm formation by Streptococcus gordonii, Fusobacterium nucleatum and Porphyromonas gingivalis was documented in vitro using growth models and scanning electron microscopy. Analysis of growth rates by species-specific 16s rRNA probes revealed distinct, early advantages to consortium growth for S. gordonii and F. nucleatum with P. gingivalis, while P. gingivalis upregulated its short mfa1 fimbriae, leading to increased invasion of DCs. F. nucleatum was only taken up by DCs when in consortium with P. gingivalis. Mature consortium regressed DC maturation upon uptake, as determined by flow cytometry. Analysis of dental plaques of PD and healthy subjects by 16s rRNA confirmed oral colonization with consortium members, but DC hematogenous spread was limited to P. gingivalis and F. nucleatum. Expression of P. gingivalis mfa1 fimbriae was increased in dental plaques and hematogenous DCs of PD patients. P. gingivalis in the consortium correlated with an adverse clinical response in the gingiva of PD subjects. In conclusion, we have identified polymicrobial synergy in a three-species oral consortium that may have negative consequences for the host, including microbial dissemination and adverse peripheral inflammatory responses.

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Phagocytosis of bacterial strains isolated from acute dentoalveolar abscess

Cited by 8 publications

References 12 publications

Amniotic fluid neutrophils can phagocytize bacteria: A mechanism for microbial killing in the amniotic cavity

Amniotic fluid neutrophils can phagocytize bacteria: A mechanism for microbial killing in the amniotic cavity

Phagocytosis of Periodontopathogenic Bacteria by Crevicular Granulocytes Is Depressed in Progressive Periodontitis

Polymicrobial synergy within oral biofilm promotes invasion of dendritic cells and survival of consortia members

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