Phagocytosis of Escherichia coli biofilm cells with different aspect ratios: a role of substratum material stiffness

Zhao, Ye; Song, Fangchao; Wang, Hao; Zhou, Junlin; Ren, Dacheng

doi:10.1007/s00253-017-8394-2

Cited by 6 publications

(4 citation statements)

References 25 publications

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“…Guegan [ 73 ] believed that the elastic modulus of agarose hydrogels could affect the formation of cell clusters of proteins adhering to bacterial cells. Yanrui et al [ 74 ] reported that the hardness of polydimethylsiloxane (PDMS) affected the formation of E. coli biofilms. They found that shorter E. coli cells were easier to engulf than longer cells isolated from the surface of soft PDMS.…”

Section: Relationship Between Dental Materials Surface Properties And...mentioning

confidence: 99%

Interaction between microorganisms and dental material surfaces: general concepts and research progress

Ren

et al. 2023

Journal of Oral Microbiology

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Bacterial adhesion to dental materials’ surfaces is the initial cause of dental materials-related infections. Therefore, inhibiting bacterial adhesion is a critical step in preventing and controlling these infections. To this end, it is important to know how the properties of dental materials affect the interactions between microorganisms and material surfaces to produce materials without biological contamination. This manuscript reviews the mechanism of bacterial adhesion to dental materials, the relationships between their surface properties and bacterial adhesion, and the impact of bacterial adhesion on their surface properties. In addition, this paper summarizes how these surface properties impact oral biofilm formation and proposes designing intelligent dental material surfaces that can reduce biological contamination.

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Section: Relationship Between Dental Materials Surface Properties And...mentioning

confidence: 99%

Interaction between microorganisms and dental material surfaces: general concepts and research progress

Ren

et al. 2023

Journal of Oral Microbiology

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“… 32,136 Recent studies have also gathered evidence that environmental stiffness can impact other factors relevant to bacterial disease. For instance, bacteria cultured on stiff substrates are more effectively removed by macrophages via phagocytosis 137 and are less susceptible to antibiotic treatments, 138 as compared to bacteria grown on soft substrates. In a recent study by Pierrat et al , the physical interactions between E. coli and human cells were examined.…”

Section: Challenges and Future Perspectivesmentioning

confidence: 99%

“… 138 Here, bacteria invasion was correlated with the host cell's actin cytoskeleton: bacteria co-localized in regions of F-actin and high cell stress, which were more abundant on stiff substrates than soft substrates. Matrix stiffness has also been shown to regulate other host cell factors relevant to infection, such as the activity of macrophages 137 and the ability of antibiotics to accumulate inside the host cell to target bacteria. 138 …”

Section: Challenges and Future Perspectivesmentioning

confidence: 99%

Mechanobiology as a tool for addressing the genotype-to-phenotype problem in microbiology

et al. 2023

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The central hypothesis of the genotype–phenotype relationship is that the phenotype of a developing organism (i.e., its set of observable attributes) depends on its genome and the environment. However, as we learn more about the genetics and biochemistry of living systems, our understanding does not fully extend to the complex multiscale nature of how cells move, interact, and organize; this gap in understanding is referred to as the genotype-to-phenotype problem. The physics of soft matter sets the background on which living organisms evolved, and the cell environment is a strong determinant of cell phenotype. This inevitably leads to challenges as the full function of many genes, and the diversity of cellular behaviors cannot be assessed without wide screens of environmental conditions. Cellular mechanobiology is an emerging field that provides methodologies to understand how cells integrate chemical and physical environmental stress and signals, and how they are transduced to control cell function. Biofilm forming bacteria represent an attractive model because they are fast growing, genetically malleable and can display sophisticated self-organizing developmental behaviors similar to those found in higher organisms. Here, we propose mechanobiology as a new area of study in prokaryotic systems and describe its potential for unveiling new links between an organism's genome and phenome.

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“…The phagocytic index was performed as described previously. 36 The BMDMs were seeded in 24-well plates and exposed to 2.5 μg/ml EVs or PBS as a negative control overnight. Then, the FITC-expressing…”

Section: Phagocytosis Assaymentioning

confidence: 99%

Erythrocyte-derived extracellular vesicles aggravate inflammation by promoting the proinflammatory macrophage phenotype through TLR4–MyD88–NF-κB–MAPK pathway

Gao

Jin

Tan

et al. 2022

Journal of Leukocyte Biology

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Transfusion of stored erythrocytes is associated with the increased risk of morbidity and mortality in critical infections, but the mechanism is incompletely understood. Previous studies have suggested that RBC‐derived extracellular vesicles (EVs) may be potential risk factors for the occurrence of transfusion‐related immunomodulation. The purpose of our study was to evaluate the effects of RBC‐derived EVs under inflammatory conditions and explore the underlying mechanisms. In vivo, the activity of EVs was evaluated in cecal ligation and puncture (CLP)‐induced sepsis. Our results showed that EVs significantly aggravated the inflammatory response to sepsis in serum and lung tissue by promoting the production of the proinflammatory factors tumor necrosis factor‐α (TNF‐α）‐interleukin‐6(IL‐6), and interleukin‐1β (IL‐1β) and reduced the survival rate of septic mice in vivo. Importantly, adoptive transfer of EVs‐pretreated bone marrow‐derived macrophages (BMDMs) obviously aggravated systemic proinflammatory factors in mice after CLP surgery. In vitro, the proinflammatory properties of EVs were shown to elevate TNF‐α, IL‐6, and IL‐1β levels in lipopolysaccharide (LPS)‐stimulated BMDMs. Moreover, EVs promoted LPS‐induced macrophage polarization into a proinflammatory phenotype. The underlying mechanism might involve EV‐mediated up‐regulation of TLR4–MyD88–NF‐κB–MAPK activity to favor macrophage cytokine production.

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Phagocytosis of Escherichia coli biofilm cells with different aspect ratios: a role of substratum material stiffness

Cited by 6 publications

References 25 publications

Interaction between microorganisms and dental material surfaces: general concepts and research progress

Interaction between microorganisms and dental material surfaces: general concepts and research progress

Mechanobiology as a tool for addressing the genotype-to-phenotype problem in microbiology

Erythrocyte-derived extracellular vesicles aggravate inflammation by promoting the proinflammatory macrophage phenotype through TLR4–MyD88–NF-κB–MAPK pathway

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