Phagocytosis of wear debris by osteoblasts affects differentiation and local factor production in a manner dependent on particle composition

Lohmann, Christoph H.; Schwartz, Zvi; Köster, Georg; Jahn, U; Buchhorn, Gottfried; MacDougall, Mary; Casasola, David; Liu, Y; Sylvia, V. L.; Dean, David D.; Boyan, Barbara D.

doi:10.1016/s0142-9612(99)00211-2

Cited by 161 publications

(152 citation statements)

References 34 publications

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“…Endotoxin contamination of particles was excluded by limulus assay (QCL-1000 Chromogenic LAL, BioWhittaker, Emerainville, France). When compared to previous in vitro studies [8,10,27], the present particle concentration can be considered as low. Comparison with in vivo situation is difficult to perform.…”

Section: Ti Particlesmentioning

confidence: 69%

The influence of wear particles in the expression of osteoclastogenesis factors by osteoblasts

Pioletti

Kottelat

2004

Biomaterials

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Orthopedic implant failures are often associated with peri-implant osteolysis. Particles generated from the wear process have been suspected to play an important role in this situation. Indeed, the peri-implant osteolysis could be due to the presence of particles stimulating the osteoclastogenesis process. We hypothesize then that the presence of a low particle concentration positively influences osteoblasts to produce osteoclastogenesis factors. If true, this hypothesis would then support the idea that the particles could be at the origin of the process leading to implant loosening. To check the validity of this hypothesis, we quantified in vitro the production of different genes involved in the osteoclastogenesis process using primary isolated human osteoblasts treated or not with particles. Results showed that low concentrations of particles might have a stimulating effect on osteoblasts to produce osteoclastogenesis factors as demonstrated by the increase of RANKL and CSF-1 gene expression in the particle group. r

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Section: Ti Particlesmentioning

confidence: 69%

The influence of wear particles in the expression of osteoclastogenesis factors by osteoblasts

Pioletti

Kottelat

2004

Biomaterials

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“…35 Human primary osteoblasts are capable of particle phagocytosis, which has been shown to lead to altered gene expression. 60,62,65,66 However, other studies have demonstrated that increased chemokine secretion also occurs independent of phagocytosis, suggesting that particle-cell contact and/or exposure to ionic dissolution products from the wear debris may have a role to play in periprosthetic osteolysis. 12,62 Our study clearly shows that cobalt in ion form leads to increased chemokine secretion and reduced osteoblast function.…”

Section: Discussionmentioning

confidence: 99%

Cobalt ions induce chemokine secretion in primary human osteoblasts

Queally¹,

Devitt²,

Butler³

et al. 2009

Journal Orthopaedic Research

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Chemokines are major regulators of the inflammatory response and have been shown to play an important role in periprosthetic osteolysis. Titanium particles have previously been shown to induce IL-8 and MCP-1 secretion in osteoblasts. These chemokines result in the chemotaxis and activation of neutrophils and macrophages, respectively. Despite a resurgence in the use of cobalt-chromium-molybdenum alloys in metal-on-metal arthroplasty, cobalt and chromium ion toxicity in the periprosthetic area has been insufficiently studied. In this study we investigate the in vitro effect of cobalt ions on primary human osteoblast activity. We demonstrate that cobalt ions rapidly induce the protein secretion of IL-8 and MCP-1 in primary human osteoblasts. This elevated chemokine secretion is preceded by an increase in the transcription of the corresponding chemokine gene. Using a Transwell migration chemotaxis assay we also demonstrate that the chemokines secreted are capable of inducing neutrophil and macrophage migration. Furthermore, cobalt ions significantly inhibit osteoblast function as demonstrated by reduced alkaline phosphatase activity and calcium deposition. In aggregate these data demonstrate that cobalt ions can activate transcription of the chemokine genes IL-8 and MCP-1 in primary human osteoblasts. Cobalt ions are not benign and may play an important role in the pathogenesis of osteolysis by suppressing osteoblast function and stimulating the production and secretion of chemokines that attract inflammatory and osteoclastic cells to the periprosthetic area. ß

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“…The wear debris is phagocytosed by macrophages and once in the acidic intramedullary environment of the lysosomes, the cobalt-chrome particles are subject to corrosion, producing high intracellular levels of ions, which can cause cell death. It has been shown that, following phagocytosis of metal particles, the osteoblastic activity of the cell is impaired which may contribute to the cellular events that occur during aseptic loosening and softtissue destruction [18,20] . Metal wear debris may also cause DNA damage and genomic instability.…”

Section: Unique Failure Modes In Mom Thamentioning

confidence: 99%

Management of failed metal-on-metal total hip arthroplasty

Griffin

D’Apuzzo²,

Browne³

2012

WJO

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The theoretical advantages of metal-on-metal (MOM) bearing couples in total hip arthroplasty (THA) have been recently balanced by concerns regarding adverse local and systemic effects. Higher than anticipated early revision rates have been reported by several joint registries. Failed MOM hips present with a spectrum of symptoms and findings and traditional methods of failure must be considered in addition to the failure modes that appear to be unique to the MOM bearing couple. Metal hypersensitivity and soft tissue immune reactions remain incompletely understood and require careful ongoing study. The tools available to evaluate MOM THAs and the indications for revision surgery remain to be defined. Outcomes following revision of MOM hips appear to depend on appropriate evaluation, early identification, and appropriate surgical management.

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Phagocytosis of wear debris by osteoblasts affects differentiation and local factor production in a manner dependent on particle composition

Cited by 161 publications

References 34 publications

The influence of wear particles in the expression of osteoclastogenesis factors by osteoblasts

The influence of wear particles in the expression of osteoclastogenesis factors by osteoblasts

Cobalt ions induce chemokine secretion in primary human osteoblasts

Management of failed metal-on-metal total hip arthroplasty

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