Pharmaceutical Drug Separations by HPCE: Practical Guidelines

Laughlin, G. M. Mc; Nolan, Judith A.; Lindahl, J. L.; Palmieri, Richard H.; Anderson, Kenneth W.; Morris, Sam; Morrison, J. A.; Bronzert, T J

doi:10.1080/10826079208018847

Cited by 119 publications

(22 citation statements)

References 42 publications

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“…Interestingly, the separation factor a for 1-phenylethanol was the same at all buffer concentrations (a = 1.030 f O.OOS), but an increase of the buffer concentration from 2.5 mM to 50 mM resulted in a reduced K of the analytes and, consequently, in a decrease of R, from 1.51 (2.5 mM) to 1.16 (50 mM) [15]. As a compromise, a buffer of medium concentration (20 mM) was chosen to facilitate changes from low to higher voltages.…”

Section: S Mayer and V Schurigmentioning

confidence: 92%

“…Here, just as with achiral conditions, high buffer concentrations lower the mobility of the analytes [15], whereas at low buffer concentrations the current is strongly reduced (2.5 m~/ 3 PA; 20 mrd10 pA; 50 m~/ 4 2 pA, measured with Chirasil-P-Dex at 30 kV). Interestingly, the separation factor a for 1-phenylethanol was the same at all buffer concentrations (a = 1.030 f O.OOS), but an increase of the buffer concentration from 2.5 mM to 50 mM resulted in a reduced K of the analytes and, consequently, in a decrease of R, from 1.51 (2.5 mM) to 1.16 (50 mM) [15].…”

Section: S Mayer and V Schurigmentioning

confidence: 93%

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Enantiomer separation using mobile and immobile cyclodextrin derivatives with electromigration

Mayer¹,

Schurig²

1994

Electrophoresis

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The principle of electrochromatography is applied to enantiomer separation with wall-coated capillaries of 50 microns (ID). As chiral stationary phase an immobilized polysiloxane containing chemically bonded monokis-6-O-octamethylene-permethyl-cyclodextrin (beta- or gamma-Chirasil-Dex) was used. The results are compared with the conventional method of adding cyclodextrin derivatives as chiral pseudostationary phase to the running buffer.

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Section: S Mayer and V Schurigmentioning

confidence: 92%

Section: S Mayer and V Schurigmentioning

confidence: 93%

Enantiomer separation using mobile and immobile cyclodextrin derivatives with electromigration

Mayer¹,

Schurig²

1994

Electrophoresis

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“…Because addition of organic solvents to the running buffers changes the zeta potential, viscosity, dielectric constant, and permittivity, and can improve the selectivity, resolution, and peak symmetry [33,34], several were used to optimize the separation of isomeric compounds which were not resolved by use of the coelectroosmotic aqueous systems [10,18,19,23].…”

Section: Effect Of Various Organic Solventsmentioning

confidence: 99%

Evaluation of coelectroosmotic capillary zone electrophoresis for the rapid analysis of twelve aromatic sulphonate compounds

1999

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SummaryCoelectroosmotic capillary zone electrophoresis (CZE) has been investigated as a means of rapid analysis of twelve aromatic sulphonate compounds. The main factors affecting reversal of electroosmotic flow (EOF) -type of osmotic modifier and concentration -were studied. Two types of osmotic modifier, an alkylammoniun salt (cetyltrimethylammonium bromide, CTAB) and a cationic polyelectrolyte (hexadimetrine bromide, HDB) were investigated. The composition of the running buffers was optimized according to the characteristics of each osmotic modifier. A concentration of HDB as low as 0.0001% (w/v) was used successfully to provide a stable and reversed EOF.

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“…Third, the use of buffer solutions with high ionic strength can produce undesired effects related to the generation of heat. If the heat is not properly dissipated by the capillary, the viscosity of the buffer and the electrophoretic mobility of the analytes can be modified, bringing about variations in the efficiency and resolution of the separations [6,15,391. In an extreme case, the high temperatures inside the capillary can produce the denaturation of the biopolymers [40].…”

Section: Peptide Behavior Depending On Ionic Strength Of Buffermentioning

confidence: 99%

Effect of pH and ionic strength of running buffer on peptide behavior in capillary electrophoresis: Theoretical calculation and experimental evaluation

Cifuentes

Poppe

1995

Electrophoresis

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The effect of pH and ionic strength of running buffer on peptide behavior in capillary electrophoresis (CE) is studied. A system for predictions of peptide migration in CE (SPPMCE) developed in our laboratory has been tested in a wide range of pH and buffer concentrations. The SPPMCE consists of a computer program for calculating peptide pKa values, an equation which relates peptide structures to their electrophoretic mobilities and a coupled computer program for the prediction of electropherograms. More than 25 different buffers have been employed, covering a pH range of 2-11 and a concentration range of 5-100 mM. Results from experiments are compared with the theoretical predictions. Good agreement is observed, which confirms the utility of the SPPMCE and allows fast and easy optimization of peptide separations in CE, with nothing more than the amino acid sequence of the linear peptide as the input.

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Pharmaceutical Drug Separations by HPCE: Practical Guidelines

Cited by 119 publications

References 42 publications

Enantiomer separation using mobile and immobile cyclodextrin derivatives with electromigration

Enantiomer separation using mobile and immobile cyclodextrin derivatives with electromigration

Evaluation of coelectroosmotic capillary zone electrophoresis for the rapid analysis of twelve aromatic sulphonate compounds

Effect of pH and ionic strength of running buffer on peptide behavior in capillary electrophoresis: Theoretical calculation and experimental evaluation

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