Pharmaceutical Polymeric Nanoparticles Prepared by the Double Emulsion- Solvent Evaporation Technique

Piñón-Segundo, Elizabeth; Nava-Arzaluz, María Guadalupe; Lechuga‐Ballesteros, David

doi:10.2174/187221112802652606

Cited by 31 publications

(17 citation statements)

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“…As gas molecules are able to enter the microcapsule by molecular diffusion or osmosis, an internal, hollow microbubble of gas is eventually obtained (17). The double emulsion solvent evaporation method is simple, requires mild reaction conditions, and it is possible to adjust the preparation of the micro bubble particle size, uniform and controllable particle size, shell properties and internal structure through material selection and preparation technology (18,19). Thus, suitable polymer nanobubbles were obtained.…”

Section: Discussionmentioning

confidence: 99%

Au-poly(lactic-co-glycolic) acid complex nanoparticles as ultrasound contrast agents: Preparation, characterization and inï¿½vitro study

et al. 2017

Mol Med Report

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The present study aimed to prepare and characterize an Au-poly(lactic-co-glycolic) acid (PLGA) complex, surfaced with gold nanoparticles (GNPs), and to assess its use as an ultrasound contrast agent for in vitro imaging. PLGA nanoparticles (NPs) were prepared by a modified double‑emulsion solvent evaporation method. GNPs were prepared via reduction with sodium citrate. Au‑PLGA complexes were created through the binding of polyethylene glycosylated GNPs to the surface of PLGA NPs. Various physical characteristics of PLGA NPs, GNPs and Au‑PLGA complexes, including morphology, size, dispersion, ζ potential and stability were observed and measured. The study of the in vitro imaging capability of the Au‑PLGA complex was conducted by setting multi‑parameter imaging conditions. The average sizes of PLGA NPs, GNPs and the Au‑PLGA complex were 144.5±30.28, 21.42±1.56 and 359.4±67.94 nm, respectively. Transmission electron microscopy (TEM) revealed that PLGA NPs and GNPs were spherically shaped, but GNPs had smooth surface while PLGA NPs had a capsular morphology with a wrinkled surface and large voids. The maximum absorption peak of 20 nm GNPs was 508 nm while that of the Au‑PLGA complex was 515 nm on an ultraviolet‑visible absorption spectrum curve. The results of TEM and the redshift spectrum demonstrated that GNPs successfully bound to the surface of the PLGA NPs. The complex demonstrated good stability and its size changed little over time. In vitro imaging experiments revealed that the Au‑PLGA complex had a good imaging ability. The present study demonstrated that the Au‑PLGA complex is a promising novel ultrasound contrast agent.

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Section: Discussionmentioning

confidence: 99%

Au-poly(lactic-co-glycolic) acid complex nanoparticles as ultrasound contrast agents: Preparation, characterization and inï¿½vitro study

et al. 2017

Mol Med Report

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“…Murine models of clefting have been widely used based upon the fact that mice and humans share similar craniofacial developmental process [3,4] Genes encoding secreted growth factors and their receptors (such as the Tgf- β eta [24,25] /Bmp superfamily [12,26], Fgf [27], Shh [4,28] and their effectors, Wnt and Lef [29]) have been shown to be essential for the normal development of head and face structures in mice. Likewise, transcription factors (such as homeobox genes [29], bHLH-related genes [29], Paired-box genes [29], Winged Helix/Forkhead [29] and Pbx [11,12]) have been found to be crucial in initiating patterns of gene expression that result in morphological changes in the developing face and head.…”

Section: Discussionmentioning

confidence: 99%

Toward Microsurgical Correction of Cleft Lip Ex Utero through Restoration of Craniofacial Developmental Programs

Dong

Landford

Hart

et al. 2017

Plastic &Amp; Reconstructive Surgery

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Background Cleft Lip with or without Palate (CL/P) is present in approximately 1 in 500–700 live births, representing the most common congenital craniofacial anomaly. Previously, we developed a unique murine model with compound Pbx deficiency that exhibits fully penetrant CL/P. To investigate the possibility of tissue repair at an early gestational stage, we designed a minimally invasive surgical approach suitable for intrauterine repair using Wnt9b-soaked collagen microspheres to restore craniofacial developmental programs for cleft correction. Methods Collagen microspheres with diameters ranging from 20–50 microns were fabricated to serve as a delivery vehicle for Wnt9b. At gestational day 11.5, wild type and Pbx-deficient murine embryos were isolated. Microspheres soaked in murine purified Wnt9b protein were microsurgically implanted at the midface λ junction. Embryos were cultured in a 37°C modified Whole Embryo Culture (WEC) system. Results Targeted release of Wnt9b resulted in augmented Wnt expression at the λ junction. Microsurgical implantation of Wnt9b-soaked microspheres resulted in cleft correction in 27.1% of the Pbx-deficient embryos. The difference in the ratio of the areas of clefting between implanted and non-implanted embryos was significant (p<0.05). Conclusion Ex utero correction of CL/P in our murine model via microsurgical intervention and targeted delivery of Wnt proteins is an innovative and promising strategy. Although further refinement and optimization of this technique will be required to improve efficacy, we believe that this approach will open new avenues towards unconventional prenatal interventions for patients with CL/P, as well as provide future approaches for prenatal repair of other congenital head and neck disorders.

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“…10 Drug (400 mg) and HPMC (100 mg) was dissolved in 10 ml of DCM and methanol (solvent) (5 ml) at room temperature. This solution was poured into fixed amount (50 ml) of non-solvent (Water) containing SLS (100 mg) as surfactant stabilizer at the same temperature.…”

Section: Methods Of Preparationmentioning

confidence: 99%

Solubility and Dissolution Enhancement of Meropenem by Nano Suspension Approach

chirumamilla¹,

Padasala²,

Aravally³

et al. 2017

JYP

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Objective: To prepare and evaluate the suitable nanosuspensions of Meropenem (BCS-IV drug) to increase its solubility and dissolution. Methodology: The meropenem nanosuspensions were prepared by emulsification solvent evaporation technique by applying ultrasonic energy through probe sonicator, where the organic phase of drug solution in methanol was emulsified in aqueous phase containing hydroxy propyl methyl cellulose as solubilizer and sodium lauryl sulphate as stabilizer. The prepared nanosuspensions were characterised for particle size, zeta potential, surface morphology by SEM, drug excipient compatibility by FTIR and DSC and conducted in-vitro drug release studies. Results: Results showed that the prepared nanosuspensions were having particle size range from 1 to 1000nm and the zeta potential from -10 to -20 mVs. Scanning electron microscopic pictures revealed that the obtained nanosuspension particles were spherical in shape with surface smoothness and in-vitro drug release studies notified that the prepared nanosuspensions showed increase in solubility and dissolution of meropenem when compared with the pure form. Conclusion: The nanosuspensions of meropenem could be successfully prepared and can be concluded that the nanosuspension formulation is a promising approach to increase the solubility and dissolution of BCS-IV drugs like meropenem.

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Pharmaceutical Polymeric Nanoparticles Prepared by the Double Emulsion- Solvent Evaporation Technique

Cited by 31 publications

References 0 publications

Au-poly(lactic-co-glycolic) acid complex nanoparticles as ultrasound contrast agents: Preparation, characterization and inï¿½vitro study

Au-poly(lactic-co-glycolic) acid complex nanoparticles as ultrasound contrast agents: Preparation, characterization and inï¿½vitro study

Toward Microsurgical Correction of Cleft Lip Ex Utero through Restoration of Craniofacial Developmental Programs

Solubility and Dissolution Enhancement of Meropenem by Nano Suspension Approach

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