The genus Bacillus is a widely distributed and prominent species that has been extensively studied for its numerous benefits in various industries, medical applications, and agriculture. In this study, endophytic Bacillus species were isolated from seven different algal species using various growth media such as Nutrient agar, starch casein agar, ISP 2, and ISP 4. Throughout the study period, a total of 36 bacterial strains with similar morphological characteristics were identified. Among the isolates, five bacterial strains were found to be predominant when grown on ISP 2 media. These five strains were further subjected to 16s RNA Sequencing to determine their taxonomic classification. The results revealed that all of these strains belonged to the genus Bacillus. However, the other endophytic strains could not be identified due to limited sequence homology with existing entries in the NCBI database. To gain further insights into the isolated Bacillus cultures, analytical studies were conducted. Firstly, the functional groups present in the cultures were identified using Fourier-transform infrared spectroscopy (FTIR). Additionally, the compounds present in the crude extracts of the cultures were analyzed using gas chromatography-mass spectrometry (GC-MS). The findings of this current research demonstrate the presence of Bacillus species among green and brown algae in the Palk Bay coastal range. Moreover, these Bacillus strains exhibit potential for various biotechnological applications. The study aims to establish the predominance of Bacillus species in the algae of the Palk Bay region in South India, thereby contributing to our understanding of the microbial ecology in this specific geographical area