Pharmacodynamic Study of Polymerized Porcine Hemoglobin (pPolyHb) in a Rat Model of Exchange Transfusion

Abstract

The objective of the present study is to evaluate the pharmacodynamic properties of polymerized porcine hemoglobin (pPolyHb) in an exchange transfusion model. Each of two groups of rats received a volume of pPolyHb or hetastarch that equalled 120-140% of estimated total blood volume (70 ml/kg) exchange transfusion. The results showed pPolyHb retained hemodynamic

Pretreatment with pPolyHb attenuates H2O2-induced endothelial cell injury through inhibition of JNK/p38 MAPK pathway by upregulation of heme oxygenase-1

“…Glutaraldehyde-polymerized porcine hemoglobin (pPolyHb) was prepared as previously described (Zhu et al 2011a, Zhu et al 2011b). pPolyHb (10.5 Ϯ 0.5 g/dL polymerized porcine hemoglobin, methemoglobin Ͻ 5%, endotoxin Ͻ 1.0 EU/mL, osmolality 300 -330 mOsm, pH 7.4 Ϯ 0.05, average molecular weight of pPolyHb 600 Ϯ 50 kD, 64 kD tetramer Ͻ 2%) was formulated and stored at 4 ° C under nitrogen gas until use.…”

Immune Safety Evaluation of Polymerized Porcine Hemoglobin (pPolyHb): A Potential Red Blood Cell Substitute

“…pPolyHb has been studied for years in our group; it shows a good potential for tissue oxygenation and survival benefit in rat models [11], but pPolyHb' effects on host immune responses are not well described.…”

“…However, in our earlier work we demonstrated that pPolyHb maintained hemodynamic stability well and wouldn't cause hypertension in an exchange transfusion rat model in that 120-140% of estimated total blood volume was replaced by pPolyHb. Furthermore, pPolyHb increased tissue oxygen, reversed anaerobic metabolism, and provided for effective life-sustaining ability [11].…”

Effects of resuscitation with polymerized porcine hemoglobin (pPolyHb) on hemodynamic stability and oxygen delivery in a rat model of hemorrhagic shock

“…We have previously demonstrated that pPolyHb can alleviate inflammatory symptoms caused by hemorrhagic shock (Zhu et al 2011b). Additionally, isovolumic exchange transfusion experiments in rats demonstrated that pPolyHb helps maintain hemodynamic stability, enables a suitable level of oxygen delivery to tissues, and effectively reverses anaerobic metabolism caused by extensive blood volume exchange (Zhu et al 2011a).…”