Pharmacodynamics of cisplatin-loaded PLGA nanoparticles administered to tumor-bearing mice

Moreno, Daniel Antunes; Zalba, Sara; Navarro, Íñigo; Ilarduya, Conchita Tros de; Garrido, María J.

doi:10.1016/j.ejpb.2009.10.005

Cited by 67 publications

(46 citation statements)

References 33 publications

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“…In addition, efficiency and specific load increased notably by the NAC presence in the nonsolvent inside the nanoparticles, which may result from the diminishing of the NAC concentration gradient. Other authors report the addition of active compound to the nonsolvent as a strategy to increase the entrapment [46,47]. For example, Avgoustakis et al report similar values for cisplatin entrapment, which increases from 0.1% to 0.66% when saturating the nonsolvent [46].…”

Section: Nac Concentration In the Nonsolventsupporting

confidence: 48%

Improvement of N-Acetylcysteine Loaded in PLGA Nanoparticles by Nanoprecipitation Method

Lancheros

Guerrero

Godoy-Silva

2018

Journal of Nanotechnology

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N-Acetylcysteine (NAC) is a hydrophilic compound with a low bioavailability. It has been used as an effective antioxidant agent.is research seeks to enhance the entrapment of NAC in PLGA nanoparticles for drug delivery systems. e nanoparticles were made using the nanoprecipitation method and changing the following parameters: the solvent/nonsolvent nature, its viscosity, pH, NAC addition to the nonsolvent, the polymer concentration and molecular weight, and NAC concentration in the solvent.e results showed that an increase in the nonsolvent viscosity produces NAC concentration in the solvent, and the nonsolvent rises its entrapment in the nanoparticles. Nanoparticles with 235.5 ± 11.4 nm size with an entrapment efficiency of 0.4 ± 0.04% and a specific load of 3.14 ± 0.33% were obtained. e results suggest that besides efficiently entrapping hydrophobic compounds, the nanoprecipitation method also has a high potential as an alternative entrapment method for hydrophilic compounds as well. However, its use in the pharmaceutical industry, as a proper specific load vehicle, still depends on the improvement of the load capacity.

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Section: Nac Concentration In the Nonsolventsupporting

confidence: 48%

Improvement of N-Acetylcysteine Loaded in PLGA Nanoparticles by Nanoprecipitation Method

Lancheros

Guerrero

Godoy-Silva

2018

Journal of Nanotechnology

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“…The authors demonstrated the antitumor activity of the system by a reduction in tumor growth in SCID (severe combined immunodeficiency) mice with HT29 colon adenocarcinoma tumors, showing higher survival rates compared to the treatment with free cisplatin [446]. In another work with a similar loading of cisplatin to PLGA nanoparticles to avoid its side effects without decreasing the drug efficacy, Moreno et al [449] used the nanoparticulate formulation in tumor-bearing mice, decreasing the tumor volume and activating apoptosis, leading to a significant antitumor activity with lower side effects than conventional cisplatin.…”

Section: Formulations Loaded With Cisplatinmentioning

confidence: 97%

Advanced targeted therapies in cancer: Drug nanocarriers, the future of chemotherapy

Pérez-Herrero

Fernández‐Medarde

2015

European Journal of Pharmaceutics and Biopharmaceutics

1,465

754

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Cancer is the second worldwide cause of death, exceeded only by cardiovascular diseases. It is characterized by uncontrolled cell proliferation and an absence of cell death that, except for hematological cancers, generates an abnormal cell mass or tumor. This primary tumor grows thanks to new vasculatization and, in time, adquires metastatic potential and spreads to other body sites, which causes metastasis and finally death. Cancer is caused by damage or mutations in the genetic material of the cells due to environmental or inherited factors. While surgery and radiotherapy are the primary treatment used for local and non-metastatic cancers, anti-cancer drugs (chemotherapy, hormone and biological therapies) are the choice currently used in metastasic cancers. Chemotherapy is based on the inhibition of the division of rapidly growing cells, which is a characteristic of the cancerous cells, but unfortunately, it also affects normal cells with fast proliferation rates, like the hair follicles, bone marrow and gastrointestinal tract cells, generating the characteristic side effects of chemotherapy. The indiscriminate destruction of normal cells, the toxicity of conventional chemotherapeutic Código de campo cambiado

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“…Cis-loaded poly(lactic-co-glycolic acid) NPs exhibit reduced side effects without compromising drug efficacy in tumor-bearing mice. 50,51 Similarly, Cis conjugated to polyethylene glycolated single-walled carbon nanotubes is effective against head and neck tumor xenografts in mice. 52 Altogether, the combination of Cis and rGO-AgNPs showed greater sensitivity than single treatment in HeLa cells.…”

Section: Combination Effect Of Cis and Rgoagnps On Cell Viability Andmentioning

confidence: 99%

Combination of graphene oxide–silver nanoparticle nanocomposites and cisplatin enhances apoptosis and autophagy in human cervical cancer cells

Yuan¹,

Gurunathan²

2017

IJN

116

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Background Cisplatin (Cis) is a widely used chemotherapeutic drug for treating a variety of cancers, due to its ability to induce cell death in cancer cells significantly. Recently, graphene and its modified nanocomposites have gained much interest in cancer therapy, due to their unique physicochemical properties. The objective of this study was to investigate the combination effect of Cis and a reduced graphene oxide–silver nanoparticle nanocomposite (rGO-AgNPs) in human cervical cancer (HeLa) cells. Materials and methods We synthesized AgNPs, rGO, and rGO-AgNP nanocomposites using C-phycocyanin. The synthesized nanomaterials were characterized using various analytical techniques. The anticancer properties of the Cis, rGO-AgNPs, and combination of Cis and rGO-AgNPs were evaluated using a series of cellular assays, such as cell viability, cell proliferation, LDH leakage, reactive oxygen species generation, and cellular levels of oxidative and antioxidative stress markers such as malondialdehyde, glutathione, SOD, and CAT. The expression of proapoptotic, antiapoptotic, and autophagy genes were measured using real-time reverse-transcription polymerase chain reaction. Results The synthesized AgNPs were well dispersed, homogeneous, and spherical, with an average size of 10 nm and uniformly distributed on graphene sheets. Cis, GO, rGO, AgNPs, and rGO-AgNPs inhibited cell viability in a dose-dependent manner. The combination of Cis and rGO-AgNPs showed significant effects on cell proliferation, cytotoxicity, and apoptosis. The combination of Cis and rGO-AgNPs had more pronounced effects on the expression of apoptotic and autophagy genes, and also significantly induced the accumulation of autophagosomes and autophagolysosomes, which was associated with the generation of reactive oxygen species. Conclusion Our findings substantiated rGO-AgNPs strongly potentiating Cis-induced cytotoxicity, apoptosis, and autophagy in HeLa cells, and hence rGO-AgNPs could be potentially applied to cervical cancer treatment as a powerful synergistic agent with Cis or any other chemotherapeutic agents.

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Pharmacodynamics of cisplatin-loaded PLGA nanoparticles administered to tumor-bearing mice

Cited by 67 publications

References 33 publications

Improvement of N-Acetylcysteine Loaded in PLGA Nanoparticles by Nanoprecipitation Method

Improvement of N-Acetylcysteine Loaded in PLGA Nanoparticles by Nanoprecipitation Method

Advanced targeted therapies in cancer: Drug nanocarriers, the future of chemotherapy

Combination of graphene oxide–silver nanoparticle nanocomposites and cisplatin enhances apoptosis and autophagy in human cervical cancer cells

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Pharmacodynamics of cisplatin-loaded PLGA nanoparticles administered to tumor-bearing mice

Cited by 67 publications

References 33 publications

Improvement of N-Acetylcysteine Loaded in PLGA Nanoparticles by Nanoprecipitation Method

Improvement of N-Acetylcysteine Loaded in PLGA Nanoparticles by Nanoprecipitation Method

Advanced targeted therapies in cancer: Drug nanocarriers, the future of chemotherapy

Combination of graphene oxide&ndash;silver nanoparticle nanocomposites and cisplatin enhances apoptosis and autophagy in human cervical cancer cells

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Combination of graphene oxide–silver nanoparticle nanocomposites and cisplatin enhances apoptosis and autophagy in human cervical cancer cells