Pharmacokinetic Characterization of Calcium from Three Calcium Salts (Calcium Chloride, Calcium Acetate and Calcium Ascorbate) in Mice

Ueda, Yasuyuki; Taira, Zenei

doi:10.2485/jhtb.21.291

Cited by 4 publications

(11 citation statements)

References 33 publications

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“…Subjects We used DBA/2 mice in this study, as DBA/2 mice rapidly express a robust sensitization to EtOH. 6,35,36) Sixweek-old DBA/2 CrSlc male mice (Japan SLC, Inc., Hamamatsu, Japan) were acclimated to the animal facility for 2 weeks before testing. The animals were group-housed (6 per cage) with ad libitum access to distilled water and standard chow (CRF-1, Charles River Laboratories, Yokohama, Japan).…”

Section: Methodsmentioning

confidence: 99%

Effects of Oral Calcium Dosage and Timing on Ethanol-Induced Sensitization of Locomotion in DBA/2 Mice

Shimizu

Mitani

Tsuchiya

et al. 2018

Biological & Pharmaceutical Bulletin

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Ethanol (EtOH) dosage, frequency, and paired associative learning affect the risk of alcoholism. Recently, Spanagel et al. reported that acamprosate calcium (Acam Ca) prescribed for alcoholism exerts an anti-relapse effect via Ca. Ca is contained in foods, sometimes consumed with alcohol. Therefore, we investigated the association among oral Ca ingestion, EtOH-induced locomotor sensitization, and plasma Ca levels on how to consume Ca for moderate drinking. We used DBA/2 CrSlc mice, and CaCl as water-soluble Ca salts. For pre-administration, elemental Ca (50, 75, 100, or 150 mg/kg, per os (p.o.)) or water for control was administered 1 h before EtOH (2 g/kg, 20 v/v (%) EtOH in saline) administration intraperitoneal (i.p.) for locomotor sensitization or for plasma Ca level changes. For post-administration, elemental Ca (100 mg/kg) was administered 1 h after EtOH. Moreover, we employed bepridil and the dopamine D1 antagonist, SCH-23390 to further examine the mechanism of EtOH-induced sensitization. The locomotor sensitization segmentalized for 300 s had two peaks (0-90 s and 180-300 s). Pre-administration of Ca (50, 75, and 100 mg/kg) significantly reduced the 0-90-s peak, selectively blocked by SCH-23390, but "non-dose dependently" as Ca 150 mg/kg did not have this effect. Bepridil blocked the suppressive effect of pre-administration of Ca (100 mg/kg). The effective pre-doses of Ca (50-100 mg/kg) maintained plasma Ca basal levels against EtOH-induced decrease of Ca. On the contrary, post-administration of Ca inversely led to significant promotion of sensitization of both locomotor peaks. Oral Ca intake had diverse effects on EtOH-induced sensitization depending on Ca dosage and timing.

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Section: Methodsmentioning

confidence: 99%

Effects of Oral Calcium Dosage and Timing on Ethanol-Induced Sensitization of Locomotion in DBA/2 Mice

Shimizu

Mitani

Tsuchiya

et al. 2018

Biological & Pharmaceutical Bulletin

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“…Even though the AUC itself is a relative measure for the extent of absorbability, in pharmacokinetic studies of drugs the absorbability is usually defined using the absolute (F abs ) or relative (F rel ) bioavailability. F abs was defined as AUC oral after an oral dose of D oral , and was normalized with AUC IV after an IV dose of D IV as follows: 22

…”

Section: Introductionmentioning

confidence: 99%

“…Thus, no one has examined comparably the absorbability among calcium salts using the absolute bioavailability of calcium by modern pharmacokinetics, and our recent report is the first study to examine supplement sources using the absolute bioavailabilities of calcium from three calcium salts: calcium chloride, calcium acetate, and calcium ascorbate, which are very soluble in water. 22 , 28 …”

Section: Introductionmentioning

confidence: 99%

“…As shown in Table 2 , the absolute bioavailability of calcium from calcium ascorbate and calcium acetate was 2.6-fold and 1.5-fold, respectively, greater than that of calcium chloride; the calcium absorbability from calcium ascorbate via the intestinal track is significantly higher than that of calcium chloride and calcium acetate. 22 Furthermore, as shown in Table 3 , Ueda et al 28 studied the effects of Hachimi-jio-gan extract on intestinal calcium absorption using pharmacokinetic calculations in an osteoporosis animal model of OVX and sham-operated (SHAM) mice. Hachimi-jio-gan is used clinically and has been shown to be effective in preventing bone loss in OVX rats.…”

Section: Introductionmentioning

confidence: 99%

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Effect of anions or foods on absolute bioavailability of calcium from calcium salts in mice by pharmacokinetics

Ueda¹,

Taira²

2013

JEP

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We studied the absolute bioavailability of calcium from calcium L-lactate in mice using pharmacokinetics, and reviewed the absolute bioavailability of calcium from three other calcium salts in mice previously studied: calcium chloride, calcium acetate, and calcium ascorbate. The results showed that calcium metabolism is linear between intravenous administration of 15 mg/kg and 30 mg/kg, and is not affected by anions. Results after oral calcium administration of 150 mg/kg showed that the intestinal absorption process was significantly different among the four calcium salts. The rank of absolute bioavailability of calcium was calcium ascorbate > calcium L-lactate ≥ calcium acetate > calcium chloride. The mean residence time (MRTab) of calcium from calcium ascorbate (32.2 minutes) in the intestinal tract was much longer than that from calcium L-lactate (9.5 minutes), calcium acetate (15.0 minutes) and calcium chloride (13.6 minutes). Furthermore, the foods di-D-fructo-furanose-1,2′:2,3′-dianhydride, sudachi (Citrus sudachi) juice, and moromi-su (a Japanese vinegar) increased the absolute bioavailability of calcium from calcium chloride by 2.46-fold, 2.86-fold, and 1.23-fold, respectively, and prolonged MRTab by 48.5 minutes, 43.1 minutes, and 44.9 minutes, respectively. In conclusion, the prolonged MRTab of calcium in the intestinal tract by anion or food might cause the increased absorbability of calcium.

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“…Sho-saiko-to extract solution (4 mg/mL, dissolved in sterile purified water) was given orally at a dose of 40 mg/kg 27) . The plasma calcium concentration was determined using calcium diagnostic kits as described previously 28) . The pharmacokinetic profile for intestinal absorption, distribution, metabolism and elimination of calcium, the area under the calcium concentration in the blood-time curve (AUC iv ), mean residence time (MRT iv ) after intravenous (i.v.)…”

Section: Pharmacokinetic Analysis Of Calcium In Micementioning

confidence: 99%

Effects of Hachimi-jio-gan Extract on Intestinal Absorption of Calcium in Ovariectomized Mice and Stimulation of RANKL-induced Osteoclast Differentiation of Raw264.7 Cells by Lipopolysaccharide

Ueda

Kanayama

Yamauchi

et al. 2012

J. Hard Tissue Biology.

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: Osteoporosis is a common metabolic bone disease often affecting postmenopausal women, and various medicines are available that attempt to treat and/or prevent this disease. Hachimi-jio-gan is a traditional oriental (Kampo) medicine used clinically, but it's mode of action is still unknown. In this study, the effects of Hachimi-jio-gan extract on intestinal calcium absorption were evaluated in an osteoporosis model using ovariectomized (OVX) and sham-operated (SHAM) mice by investigating pharmacokinetic parameters in these animals. The ability of Hachimi-jio-gan extract to inhibit lipopolysaccharide-mediated stimulation of osteoclasts in bones using receptor activator of the NF-kB ligand (RANKL)-induced osteoclast differentiation of RAW264.7 cells was also investigated. The metabolic behavior of calcium did not differ between OVX and SHAM mice as the pharmacokinetics of calcium was equivalent after intravenous administration. The absolute bioavailability of calcium indicated that the extent of intestinal calcium absorption in the OVX (10.3%) and SHAM (10.7%) mice was at similar low levels. Hachimi-jio-gan extract potentially improved the intestinal calcium absorption by 1.96-and 1.86-fold, respectively. Hachimi-jio-gan extract further suppressed the potent stimulation of RANKL-induced osteoclast differentiation in RAW264.7 cells. These results suggest that Hachimi-jio-gan extract may be suitable for treatment and prevention of osteoporosis through its ability to increase intestinal calcium absorption and suppress osteoclast differentiation.

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Pharmacokinetic Characterization of Calcium from Three Calcium Salts (Calcium Chloride, Calcium Acetate and Calcium Ascorbate) in Mice

Cited by 4 publications

References 33 publications

Effects of Oral Calcium Dosage and Timing on Ethanol-Induced Sensitization of Locomotion in DBA/2 Mice

Effects of Oral Calcium Dosage and Timing on Ethanol-Induced Sensitization of Locomotion in DBA/2 Mice

Effect of anions or foods on absolute bioavailability of calcium from calcium salts in mice by pharmacokinetics

Effects of Hachimi-jio-gan Extract on Intestinal Absorption of Calcium in Ovariectomized Mice and Stimulation of RANKL-induced Osteoclast Differentiation of Raw264.7 Cells by Lipopolysaccharide

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