Pharmacokinetic comparison of 15 active compositions in rat plasma after oral administration of raw and honey‐processed Aster tataricus extracts

Abstract

A sensitive ultra‐high‐performance liquid chromatography–tandem mass spectrometry method was developed and validated to clarify pharmacokinetic properties of 15 compounds (quercetin, isorhamnetin, chlorogenic acid, isoquercitrin, caffeic acid, scopoletin, 7‐hydroxycoumarin, shionone, ferulic acid, kaempferol‐7‐O‐β‐d‐glucopyranoside, methyl caffeate, luteolin, kaempferol, epifriedelinol, and protocatechuic acid) in raw and honey‐processed Aster tataricus. Separation was carried out on an ACQUITY UPLC®BEH C18 column (2.1 × 100 mm, 1.7 μm) using a gradient elution with mobile phase constituting 0.1% formic acid‐water and 0.05% formic acid‐methanol. Quantitative analysis was performed using multiple reaction monitoring detection in both positive and negative ionization modes. Calibration curves showed good linearity (r2 > 0.991) over the corresponding concentration range. The intra‐ and interday precisions were within 10.1%, and accuracy ranged from −11.4 to 12.4%. The extraction recoveries and matrix effects were 78.1–100.0% and 81.1–113.7%, respectively. The analytes were stable under four storage conditions with relative standard deviations less than 12.6%. The validated method was successfully applied to compare the pharmacokinetic behaviors of raw and honey‐processed Aster tataricus for the first time. The results indicated that the areas under the curve (AUCs) of shionone, ferulic acid, and protocatechuic acid in honey‐processed A. tataricus group were significantly lower than that of raw A. tataricus group.

An LC‐MS/MS method for simultaneous quantification of 11 components of Xian‐Xiong‐Gu‐Kang in the plasma of osteoarthritic rats and pharmacokinetic analysis

“…Pretreatment methods for plasma samples usually include protein precipitation, LLE [24], and SPE [21]. In this study, protein precipitation by methanol-acetonitrile was used to extract the active components from plasma samples, and the recoveries of most of the components were higher than that achieved with LLE and several other protein precipitation methods (data not shown).…”

Differences of Chemical Constituents and Pharmacological Activities of Traditional Herbs Before and After Honey-Frying

“…Several herbs such as Gan Cao, Huang Qi, Kuan Dong Hua, Ma Huang, and Zi Wan were also studied for the change in the pharmacokinetics of biologically active compounds before and after honey frying. 14,25,31,36,49 In particular, Huang Qi had also been researched to confirm increasing in Qi tonifying effect (which is vital energy-enhancing effect in terms of modern medicine) after processing. 26 Tatarinow's aster (Zi Wan)…”

“…Terpenoids, flavonoids, peptides, coumarins, anthraquinones, and organic acids 44 -Increase: Content of total flavonoid and triterpenoid (protocatechuic acid, isoquercitrin, and kaempferol-7-O-β-D-glucopyranoside) 47 -Increase: Anti-inflammatory effect 48 -Decrease: Content of phenolic aicds (chlorogenic acid, caffeic acid, 7-hydroxycoumarin, ferulic acid, kaempferol, shionon, and epifriedelinol) 46 -Decrease: bioavailability 49 -There was a difference in chemical composition of 22 nonvolatile compounds and 12 volatile compounds 45 NY: Not yet.…”

“…The results showed that the honey-roasting reduced the bioavailability of these herb ingredients. 49…”

Pharmacokinetic studies of six major 2‐(2‐phenylethyl) chromones in rat plasma using ultra high performance liquid chromatography with tandem mass spectrometry after oral administration of agarwood ethanol extract

“…Pharmacokinetic studies play an important role in explaining efficacy, toxicology of Chinese medicine, and guiding its clinical application [24,25]. Most of previous research on agarwood focused on the pharmacology and structural identification of compounds.…”