Pharmacokinetic study of benzoylmesaconine in rats using an enzyme immunoassay system

Abstract

A highly sensitive enzyme immunoassay (EIA) system for quantitative determination of benzoylmesaconine (BM) in biological samples was developed. The sensitivity of an antiserum towards bgalactosidase (b-gal)-labeled antigen was in the range of 10 -1 -10 5 ng/ml of BM in rat serum. Under the same conditions, the antiserum had quite weak cross-reactivity with related alkaloids in the aconite tuber. The coefficients of variance (CV) of intra-and interday assays were 6.4 and 10.2%, and 3.4 and 14.8% in the presence of high and low concentrations, respectively, of BM in rat serum. The concentration versus time curve of BM after intravenous administration corresponded to a two-compartment model, and that after oral administration corresponded to a one-compartment model. Benzoylmesaconine was identified as two peaks in serum at 48 h after oral administration; consequently its area under the serum concentration-time curve (AUC 0-48 ) and mean residence time (MRT) values were significantly high. Hydrolysis by intestinal

Simultaneous determination of benzoylmesaconine and piperine in rat plasma after oral administration of Naru-3 by an ultra fast liquid chromatography-tandem mass spectrometry method and its application in a comparative pharmacokinetic study

“…It could be transferred from tissues to plasma, and this might cause another absorption peak in plasma. 30 Meanwhile intrahepatic circulation could also be responsible for the appearance of the second peak. Moreover, herb powders were the experimental material in the study, and as a consequence, the constituents in deep layer dissolving from herb powders would take more time than in a surface layer.…”