Pharmacokinetic study of ofloxacin enantiomers in <i>Pagrosomus major</i> by chiral HPLC

Nie, Jing; Wang, Yangguang; Gao, Xiao‐Feng; Ouyang, Xiaolong; Yang, Li‐Ye; Di, Yu; Wu, Wenwang; Xu, Huaxi

doi:10.1002/bmc.3565

Cited by 9 publications

(9 citation statements)

References 19 publications

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“…They exhibit broad-spectrum antimicrobial activity against Gram-positive and Gram-negative pathogens and Mycoplasma spp. (Spreng, Deleforge, Thomas, Boisrame, & Drugeon, 1995;Thomas, Nicolas, Dizier, Mainil, & Linden, 2003), low toxicity, and limited development of bacterial resistance (Nie et al, 2016). Therefore, MBF and OFX are intensively applied for the treatment of various human and animal infectious diseases, such as urinary tract infection, respiratory tract infection, and osteomyelitis (Cotard et al, 1995;Potter, Illambas, Pelligand, Rycroft, & Lees, 2013;Sidhu, Landoni, Aliabadi, & Lees, 2010).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous screening for marbofloxacin and ofloxacin residues in animal-derived foods using an indirect competitive immunoassay

Chen

Guo

et al. 2017

Food and Agricultural Immunology

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A broad-specificity immunoassay was established to monitor marbofloxacin (MBF) and ofloxacin (OFX) residues in raw milk and porcine muscle samples. The main influential parameters in the assay buffer were investigated to improve the assay performance. The cross-reactivities (CRs) were lower than 0.25%, except for OFX (41.38%). The quantitative working ranges for MBF and OFX based on fortified matrices ranged from 0.11 to 30.85 ng mL −1 , with a 50% inhibitory concentration of 0.76±0.12 ng mL −1 for MBF and 2.70±0.28 ng mL −1 for OFX. The detection limits of enzyme-linked immunosorbent assay (ELISA) ranged from 0.053 to 0.358 ng mL −1 in spiked samples. The mean recoveries were in the range of 80.52-95.46%, less than 12.06% of the coefficient of variation. A good correlation between the ELISA results and those from instrument methods demonstrated the practical application of the novel ELISA. Therefore, the immunoassay proposed is feasible for MBF and OFX analysis in animal-derived foods.

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Section: Introductionmentioning

confidence: 99%

Simultaneous screening for marbofloxacin and ofloxacin residues in animal-derived foods using an indirect competitive immunoassay

Chen

Guo

et al. 2017

Food and Agricultural Immunology

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“…Acetic acid (0.2% in ethanol ( v / v )) and triethylamine (0.2% in ethanol ( v / v )) were used as additives. The detection wavelength was 294 nm, 20 μL of analyte was injected, and the column temperature was 25 °C [29]. The HPLC system was controlled and the data were analyzed using a computer equipped with ChemStation software (Rev.B.04.02, Agilent).…”

Section: Methodsmentioning

confidence: 99%

Synthesis and Characterization of Magnetic Molecularly Imprinted Polymer for the Enrichment of Ofloxacin Enantiomers in Fish Samples

et al. 2016

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Abstract:A new method for the isolation and enrichment of ofloxacin enantiomers from fish samples was developed using magnetic molecularly imprinted polymers (MMIPs). These polymers can be easily collected and rapidly separated using an external magnetic field, and also exhibit a high specific recognition for ofloxacin enantiomers. The preparation of amino-functionalized MMIPs was carried out via suspension polymerization and a ring-opening reaction using rac-ofloxacin as a template, ethylenediamine as an active group, glycidyl methacrylate and methyl methacrylate as functional monomers, divinylbenzene as a cross-linker, and Fe 3 O 4 nanoparticles as magnetic cores. The characteristics of the MMIPs were assessed using transmission electron microscopy (TEM), X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), and vibrating sample magnetometer (VSM) measurements. Furthermore, the adsorption properties were determined using Langmuir and Freundlich isotherm models. The conditions for use of these MMIPs as magnetic solid-phase extraction (MSPE) sorbents, including pH, adsorption time, desorption time, and eluent, were investigated in detail. An extraction method using MMIPs coupled with high performance liquid chromatography (HPLC) was developed for the determination of ofloxacin enantiomers in fish samples. The limits of quantitation (LOQ) for the developed method were 0.059 and 0.067 µg¨mL´1 for levofloxacin and dextrofloxacin, respectively. The recovery of ofloxacin enantiomers ranged from 79.2%˘5.6% to 84.4%˘4.6% and ofloxacin enantiomers had good linear relationships within the concentration range of 0.25-5.0 µg¨mL´1 (R 2 > 0.999). The obtained results demonstrate that MSPE-HPLC is a promising approach for preconcentration, purification, and simultaneous separation of ofloxacin enantiomers in biomatrix samples.

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“…For example, pharmacokinetic studies showed that the S-configuration of ofloxacin was 8−128 times more potent than the (R)-enantiomer in inhibiting bacterial activity. 43 SH-β-CD COF displayed chiral separation for three fluoroquinolones along which gatifloxacin was completely separated. Hence, gatifloxacin was used as a template drug to illustrate the influence of chiral site density on the separability of [SH-β-CD] x -TAPB-DVA COF.…”

Section: Molecular Modeling Study Of the Chiral Recognition Mechanismmentioning

confidence: 99%

Chirality-Controlled Mercapto-β-cyclodextrin Covalent Organic Frameworks for Selective Adsorption and Chromatographic Enantioseparation

Cao

et al. 2023

ACS Appl. Mater. Interfaces

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Chiral covalent organic frameworks (CCOFs) benefit from superior stability, abundant chiral environment, and homogeneous pore configuration. In its constructive tactics, only the post-modification method allows for the integration of supramolecular chiral selectors into achiral COFs. Here, the finding utilizes 6-deoxy-6-mercapto-β-cyclodextrin (SH-β-CD) as chiral subunits and 2,5-dihydroxy-1,4-benzenedicarboxaldehyde (DVA) as the platform molecule to synthesize chiral functional monomers through thiol-ene click reactions and directly establish ternary “pendant-type” SH-β-CD COFs. The chiral site density on SH-β-CD COFs was regulated by changing the proportion of chiral monomers to obtain an optimal construction strategy and remarkably improve the ability of chiral separation. SH-β-CD COFs were coated on the inner wall of the capillary in a covalently bound manner. The prepared open tubular capillary was achieved for the separation of six chiral drugs. By combining the outcomes of selective adsorption and chromatographic separation, we observed the higher density of chiral sites in the CCOFs, and poorer results were achieved. From the perspective of spatial conformational distribution, we interpret the variation in the performance of these chirality-controlled CCOFs for selective adsorption and chiral separation.

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Pharmacokinetic study of ofloxacin enantiomers in Pagrosomus major by chiral HPLC

Cited by 9 publications

References 19 publications

Simultaneous screening for marbofloxacin and ofloxacin residues in animal-derived foods using an indirect competitive immunoassay

Simultaneous screening for marbofloxacin and ofloxacin residues in animal-derived foods using an indirect competitive immunoassay

Synthesis and Characterization of Magnetic Molecularly Imprinted Polymer for the Enrichment of Ofloxacin Enantiomers in Fish Samples

Chirality-Controlled Mercapto-β-cyclodextrin Covalent Organic Frameworks for Selective Adsorption and Chromatographic Enantioseparation

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