Pharmacokinetics of ergosterol in rats using rapid resolution liquid chromatography–atmospheric pressure chemical ionization multi-stage tandem mass spectrometry and rapid resolution liquid chromatography/tandem mass spectrometry

Zhao, Ying-Yong; Cheng, Xian‐Long; Li, Rui; Ho, Charlene C.; Wei, Feng; Yan, Suihong; Lin, Rui‐Chao; Zhang, Yongmin; Sun, Wenli

doi:10.1016/j.jchromb.2011.05.025

Cited by 28 publications

(23 citation statements)

References 21 publications

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“…1) was best-known as a steroid, found widely in many medicinal fungi such as Polyporus umbellatus, Cordyceps sinensis and Hypsizigus marmoreus [7]. Recent studies showed that the ergosterol from P. umbellatus had a wide range of biological activities, such as diuretic activity [8], possessing cytotoxic activity Z.…”

Section: Introductionmentioning

confidence: 99%

Interaction of ergosterol with bovine serum albumin and human serum albumin by spectroscopic analysis

Cheng

2012

Mol Biol Rep

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This study was designed to examine the interactions of ergosterol with bovine serum albumin (BSA) and human serum albumin (HSA) under physiological conditions with the drug concentrations in the range of 2.99-105.88 μM and the concentration of proteins was fixed at 5.0 μM. The analysis of emission spectra quenching at different temperatures revealed that the quenching mechanism of HSA/BSA by ergosterol was the static quenching. The number of binding sites n and the binding constants K were obtained at various temperatures. The distance r between ergosterol and HSA/BSA was evaluated according to Föster non-radioactive energy transfer theory. The results of synchronous fluorescence, 3D fluorescence, FT-IR, CD and UV-Vis absorption spectra showed that the conformations of HSA/BSA altered in the presence of ergosterol. The thermodynamic parameters, free energy change (ΔG), enthalpy change (ΔH) and entropy change (ΔS) for BSA-ergosterol and HSA-ergosterol systems were calculated by the van't Hoff equation and discussed. Besides, with the aid of three site markers (for example, phenylbutazone, ibuprofen and digitoxin), we have reported that ergosterol primarily binds to the tryptophan residues of BSA/HSA within site I (subdomain II A).

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Section: Introductionmentioning

confidence: 99%

Interaction of ergosterol with bovine serum albumin and human serum albumin by spectroscopic analysis

Cheng

2012

Mol Biol Rep

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“…The results showed that, for the plasma samples, different tissues and excrement of low, intermediate and high concentrations, the extraction recovery was >75%. This was in agreement with other studies (Zhao et al ., ; Wang et al , ), indicating that the method could undergo good extraction recovery.…”

Section: Resultsmentioning

confidence: 97%

“…The accuracy of the method was 93–108%, which was comparable to the other current study (Zhao et al ., ), indicating that the method was highly accurate.…”

Section: Resultsmentioning

confidence: 98%

Simultaneous HPLC determination of ergosterol and 22,23‐dihydroergosterol in Flammulina velutipes sterol‐loaded microemulsion

Tong

Zhong

et al. 2013

Biomedical Chromatography

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An efficient HPLC method was developed and validated for the simultaneous determination of ergosterol and 22,23-dihydroergosterol in Flammulina velutipes sterol-loaded microemulsions (FVSMs). The different chromatographic conditions for in vitro and in vivo determinations were investigated, with the application examined by tissue distribution. Chromatographic separation was achieved on an Inertsil ODS-SP (250 × 4.6 mm, 5 µm) analytical column using a mobile phase of 98% methanol (in vitro), and 93% methanol for stomach samples and 96% methanol for other samples (in vivo) at 1.0 mL/min. The sterol content was detected at 282 nm. The established in vitro linearity ranges for ergosterol and 22,23-dihydroergosterol were 0.58-72.77 µg/mL (r1 = 0.9999) and 0.59-73.25 µg/mL (r2 = 0.9999), respectively, with the biological (in vivo) samples following the same trend. The accuracy of the method was >99% (in vitro) and between 93%-108% (in vivo). The LOQ was 2.15 µg/L for ergosterol and 2.41 µg/L for 22,23-dihydroergosterol in the in vitro studies. Also, the precisions met the acceptance criterion. These results indicate that the established HPLC method was specific, linear, accurate, precise and sensitive for the separation and simultaneous determination of ergosterol and 22,23-dihydroergosterol.

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“…Small particle size which lowers plate heights over a wider range of higher linear velocities, will result in better resolution and sensitivity as well as reduced analysis time. [21][22][23] Quadrupole time-of-flight mass spectrometry (Q-TOF=MS) allows the generation of mass information with higher accuracy and precision. For the identification of known or unknown compounds, these accurately measured mass values can be used to produce candidate empirical formulae which, at the mass accuracy less than 5 ppm, significantly reduce the number of possible structures of putative compounds.…”

Section: Introductionmentioning

confidence: 99%

PHARMACOKINETICS, BIOAVAILABILITY, AND METABOLISM OF 2,3,5,4′-TETRAHYDROXYSTILBENE-2-O-β-D-GLUCOSIDE IN RATS BY ULTRA-PERFORMANCE LIQUID CHROMATOGRAPHY–QUADRUPOLE TIME-OF-FLIGHT MASS SPECTROMETRY AND HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY-ULTRAVIOLET DETECTION

Zhao

Cheng

Wei

et al. 2013

Journal of Liquid Chromatography & Related Technologies

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& 2,3,5,4 0 -tetrahydroxystilbene-2-O-b-D-glucoside (THSG), a natural product purified from the Chinese medical herb Polygoni multiflori Radix, has been demonstrated to possess many pharmacological activities, including antioxidant, anti-inflammatory, hepatoprotective, and antitumor properties. Ultra-performance liquid chromatography-time-of-flight mass spectrometry (UPLC-Q-TOF=MS) and high-performance liquid chromatography-ultraviolet detection (HPLC-UV) methods were developed for the pharmacokinetics, bioavailability, absorption, and metabolism studies of THSG in rats following a single intravenous or oral administration. The metabolites (M1 and M2) were identified in plasma by UPLC-Q-TOF=MS. The concentrations of the THSG in rat plasma, and bile, urine, or feces samples were determined by HPLC-UV. The results show THSG was rapidly absorbed within 30 min in plasma. Absolute bioavailability of THSG was 40%. Total recovery of unchanged THSG within 24 hr were low (0.041% in bile, 0.06% in feces), whereas the amount of unchanged THSG excreted in the urine within 24 hr was lower than LLOQ.

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Pharmacokinetics of ergosterol in rats using rapid resolution liquid chromatography–atmospheric pressure chemical ionization multi-stage tandem mass spectrometry and rapid resolution liquid chromatography/tandem mass spectrometry

Cited by 28 publications

References 21 publications

Interaction of ergosterol with bovine serum albumin and human serum albumin by spectroscopic analysis

Interaction of ergosterol with bovine serum albumin and human serum albumin by spectroscopic analysis

Simultaneous HPLC determination of ergosterol and 22,23‐dihydroergosterol in Flammulina velutipes sterol‐loaded microemulsion

PHARMACOKINETICS, BIOAVAILABILITY, AND METABOLISM OF 2,3,5,4′-TETRAHYDROXYSTILBENE-2-O-β-D-GLUCOSIDE IN RATS BY ULTRA-PERFORMANCE LIQUID CHROMATOGRAPHY–QUADRUPOLE TIME-OF-FLIGHT MASS SPECTROMETRY AND HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY-ULTRAVIOLET DETECTION

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