Pharmacokinetics of Gentamicin C
 1
 , C
 1a
 , and C
 2
 in Beagles after a Single Intravenous Dose

Isoherranen, Nina; Lavy, Eran; Soback, S.

doi:10.1128/aac.44.6.1443-1447.2000

Cited by 29 publications

(29 citation statements)

References 21 publications

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“…Differences have also been reported in the pharmacokinetic parameters of the different gentamicin components in dogs (larger apparent volume of distribution and slower clearance of component C 1 ) [13] and horses (faster clearance of C 1a ) [15]. The differences in PK were mainly attributed to higher tissue binding for C 1 compared with others [13]. This supports the hypothesis that there are significant differences in the pharmacokinetics of gentamicin components, but these differences are not consistent across species.…”

Section: Discussionsupporting

confidence: 66%

“…Also notable was that C 1a seemed to have been poorly absorbed following SC administration. Differences have also been reported in the pharmacokinetic parameters of the different gentamicin components in dogs (larger apparent volume of distribution and slower clearance of component C 1 ) [13] and horses (faster clearance of C 1a ) [15]. The differences in PK were mainly attributed to higher tissue binding for C 1 compared with others [13].…”

Section: Discussionmentioning

confidence: 86%

“…The pharmacokinetics of individual gentamicin components were studied in dogs [13], turkeys [14] and the horse [15] after intravenous administration only. However, there is no available pharmacokinetics data in other species of animals including chicken.…”

Section: Introductionmentioning

confidence: 99%

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Pharmacokinetics of Gentamicin C after IV, IM, SC and Oral Administration , C , C 1 1a and C 2 in Broiler Chickens 2a

Abu‐Basha¹

2013

JBB

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The pharmacokinetics and bioavailability of four major gentamicin components (C 1 , C 1a , C 2 and C 2a ) in chicken plasma administered at 5 mg/kg body weight by different routes of administration (IV, IM, SC and oral) was determined using reversed-phase high performance liquid chromatography (RP-HPLC) and pre-column derivatization with Phenylisocyanate (PIC). All the components, except for C 1a were well absorbed (bioavailability of 60% or greater) following administration by the IM and SC routes. The bioavailability of C 1a was 58% and 35% following IM and SC administration, respectively. The apparent volume of distribution (V ss and Vd area ) for the C 1 component was significantly smaller than for any of the other components individually or combined. In addition, the C 1 component had a significantly shorter t½β and MRT following intravenous administration and a higher C max /Dose following intramuscular administration. This study showed significant differences in some pharmacokinetics parameters between four gentamicin components (C 1a , C 2a , C 1 and C 2 ) after administration of single mixture of gentamicin by different routes in chickens. The differences may have clinical and toxicological implications, and could explain the high variation in total gentamicin pharmacokinetics.

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Section: Discussionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 86%

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Pharmacokinetics of Gentamicin C after IV, IM, SC and Oral Administration , C , C 1 1a and C 2 in Broiler Chickens 2a

Abu‐Basha¹

2013

JBB

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“…Despite the wide therapeutic use of gentamicin for decades and the importance of gentamicin in clinical practice, chromatographic methods able to determine the individual components in biological fluids are few and limited in performance [3]. Determination of gentamicin in biological matrix has been performed using various methods, based on microbiological assay [4], enzyme immunoassay [5], polarization fluoroimmunoassay [6], gas chromatography [7], and HPLC [8][9][10][11][12]. Microbiological assays cannot be recommended for clinical analysis because of their nonspecificity and inaccuracy.…”

Section: Introductionmentioning

confidence: 99%

Gentamicin assay in human serum by solid-phase extraction and capillary electrophoresis

Kaale¹,

Long

Fonge

et al. 2005

Electrophoresis

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We describe the development of a capillary electrophoresis method for the determination of gentamicin C1, C1a, C2a, and C2 components in human serum. Using a weak cation-exchanger with 20 mM phosphate buffer, pH 7.4, 200 mM borate buffer, pH 9.0, and ammonia/methanol, solid-phase extraction (SPE) of gentamicin components from the human sera was performed. The extract was derivatized with 1,2-phthalic dicarboxaldehyde/mercaptoacetic acid reagent. The derivatives were separated with a background electrolyte comprising 60 mM 2-(N-cyclohexylamino)ethanesulfonic acid (CHES) buffer at pH 9.5 containing 31.6% m/v methanol, and quantified with UV-light absorption detection at 230 nm. The identity of the gentamicin components was confirmed by mass spectrometry. The SPE recovery of the gentamicin ranged from 78% to 93%. The calibration curves were linear from the concentration limit of quantitation (LOQ) to 30 mg/L for the gentamicin mixture. The LOQ for gentamicin C1 was 0.33 mg/L, for C2a 0.23 mg/L, C2 0.25 mg/L, C1a 0.27 mg/L and the concentration limit of detection (LOD) for C1 was 0.15 mg/L, C2a 0.11 mg/L, C2 0.12 mg/L, C1a 0.13 mg/L. Intra-assay relative standard deviation (RSD) values were for C1 (5%), C1a (7%), C2 (6.5%) and C2a (9%); inter-assay RSD values were for C1 (11%), C1a (13.3%), C2 (15%) and C2a (14%). The Pearson's correlation between capillary electrophoresis and immunoassay revealed a linear relationship between these two techniques with r = 0.9. This method for determination of gentamicin C1, C1a, C2a, and C2 in human serum can thus be used in the entire therapeutic concentrations range of gentamicin.

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“…The other component of gentamicin is C 2a , which is a 6'-C epimer of C2 (Seidl and Nerad, 1988). Several papers on high-performance liquid chromatographic (HPLC) methods (Isoherranen et al, 2000;Al-Amoud et al, 2002) with fluorometric detection have been published for the quantitative determination of the gentamicin complex consisting of closely related compounds such as C 1 , C 1a , C 2 and C 2a . Among derivatisation techniques, o-phthalaldehyde (OPA) and fluorenylmethyloxycarbonyl chloride (FMOC) are frequently applied to detect gentamicin in complex biological matrices.…”

Section: Abstract: Gentamicin Dmso Ipec-j2 Permeationmentioning

confidence: 99%

Gentamicin sulphate permeation through porcine intestinal epithelial cell monolayer

Gyetvai

Jerzsele

Pászti-Gere

et al. 2015

Acta Veterinaria Hungarica

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Gentamicin is an aminoglycoside antibiotic widely used in combination with dimethyl sulphoxide (DMSO) in topical drug formulations. It is not known, however, whether DMSO can enhance the permeation of gentamicin through biological membranes, leading to oto-and nephrotoxic side effects. A simple and reliable high-performance liquid chromatographic (HPLC) method was applied for the quantitative determination of gentamicin collected from the apical and basolateral compartments of the porcine intestinal epithelial cell line IPEC-J2 cell monolayer using fluorometric derivatisation of the analyte with fluorenylmethyloxycarbonyl chloride (FMOC) prior to chromatographic run in the presence and absence of 1% DMSO. The lack of change in transepithelial electrical resistance (TER) demonstrated that gentamicin and 1% DMSO did not affect IPEC-J2 cell monolayer integrity via the disruption of cell membranes. Chromatographic data also ascertained that gentamicin penetration across the cell monolayer even in the presence of 1% DMSO was negligible at 6 h after the beginning of apical gentamicin administration. This study further indicates that the addition of this organic solvent does not increase the incidence of toxic effects related to gentamicin permeation.

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Pharmacokinetics of Gentamicin C ₁ , C _1a , and C ₂ in Beagles after a Single Intravenous Dose

Cited by 29 publications

References 21 publications

Pharmacokinetics of Gentamicin C after IV, IM, SC and Oral Administration , C , C 1 1a and C 2 in Broiler Chickens 2a

Pharmacokinetics of Gentamicin C after IV, IM, SC and Oral Administration , C , C 1 1a and C 2 in Broiler Chickens 2a

Gentamicin assay in human serum by solid-phase extraction and capillary electrophoresis

Gentamicin sulphate permeation through porcine intestinal epithelial cell monolayer

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Pharmacokinetics of Gentamicin C 1 , C 1a , and C 2 in Beagles after a Single Intravenous Dose

Cited by 29 publications

References 21 publications

Pharmacokinetics of Gentamicin C after IV, IM, SC and Oral Administration , C , C 1 1a and C 2 in Broiler Chickens 2a

Pharmacokinetics of Gentamicin C after IV, IM, SC and Oral Administration , C , C 1 1a and C 2 in Broiler Chickens 2a

Gentamicin assay in human serum by solid-phase extraction and capillary electrophoresis

Gentamicin sulphate permeation through porcine intestinal epithelial cell monolayer

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Pharmacokinetics of Gentamicin C ₁ , C _1a , and C ₂ in Beagles after a Single Intravenous Dose