Pharmacokinetics of Photogem using fluorescence monitoring in Wistar rats

Melo, C. A. S.; Kurachi, Cristina; Grecco, Clóvis; Sibata, C; Castro‐e‐Silva, Orlando; Bagnato, V. S.

doi:10.1016/j.jphotobiol.2003.11.005

Cited by 42 publications

(46 citation statements)

References 12 publications

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“…After autofluorescence subtraction, the spectra show clearly the presence and quantity of the drug. Extracting the precise concentration of PS in the tissue using the fluorescence spectrum 13 is a challenging task due to the optical properties of the medium. Since tissue is a turbid medium, the one-to-one relation between concentration and fluorescence does not hold.…”

Section: Resultsmentioning

confidence: 99%

“…A 5 mg mL −1 ͑in 0.9% NaCl͒ stock solution of the PS was prepared and administered at a concentration of 1.5 mg/kg of body weight through the vena cava, which was made accessible by surgery. 12 Thirty minutes after Photogem® injection, 13 its concentration was confirmed to be at the maximum in the liver via fluorescence spectroscopy. The right lobe of each liver at the center position was then exposed to the light spot.…”

Section: E Pdt Proceduresmentioning

confidence: 97%

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Possibility for a full optical determination of photodynamic therapy outcome

Vollet‐Filho¹,

Menezes²,

Moriyama³

et al. 2009

Journal of Applied Physics

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The microfluidic system for studies of carcinoma and normal cells interactions after photodynamic therapy (PDT) procedures Biomicrofluidics 5, 041101 (2011) Drug injection into fat tissue with a laser based microjet injector J. Appl. Phys. 109, 093105 (2011) A near infrared instrument to monitor relative hemoglobin concentrations of human bone tissue in vitro and in vivo Rev. Sci. Instrum. 81, 043111 (2010) Mechanism of oxidative stress generation in cells by localized near-infrared femtosecond laser excitation Appl. Phys. Lett. 95, 233702 (2009) Probing nanoantenna-directed photothermal destruction of tumors using noninvasive laser irradiation Appl. Phys. Lett. 95, 233701 (2009) Additional information on J. Appl. Phys. The efficacy of photodynamic therapy ͑PDT͒ depends on a variety of parameters: concentration of the photosensitizer at the time of treatment, light wavelength, fluence, fluence rate, availability of oxygen within the illuminated volume, and light distribution in the tissue. Dosimetry in PDT requires the congregation of adequate amounts of light, drug, and tissue oxygen. The adequate dosimetry should be able to predict the extension of the tissue damage. Photosensitizer photobleaching rate depends on the availability of molecular oxygen in the tissue. Based on photosensitizers photobleaching models, high photobleaching has to be associated with high production of singlet oxygen and therefore with higher photodynamic action, resulting in a greater depth of necrosis. The purpose of this work is to show a possible correlation between depth of necrosis and the in vivo photosensitizer ͑in this case, Photogem®͒ photodegradation during PDT. Such correlation allows possibilities for the development of a real time evaluation of the photodynamic action during PDT application. Experiments were performed in a range of fluence ͑0 -450 J / cm 2 ͒ at a constant fluence rate of 250 mW/ cm 2 and applying different illumination times ͑0-1800 s͒ to achieve the desired fluence. A quantity was defined ͑ ͒ as the product of fluorescence ratio ͑related to the photosensitizer degradation at the surface͒ and the observed depth of necrosis. The correlation between depth of necrosis and surface fluorescence signal is expressed in and could allow, in principle, a noninvasive monitoring of PDT effects during treatment. High degree of correlation is observed and a simple mathematical model to justify the results is presented.

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Section: Resultsmentioning

confidence: 99%

Section: E Pdt Proceduresmentioning

confidence: 97%

Possibility for a full optical determination of photodynamic therapy outcome

Vollet‐Filho¹,

Menezes²,

Moriyama³

et al. 2009

Journal of Applied Physics

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“…Therefore, the type II response is used mainly in photodynamic therapy. However, Ochsner reported that type II responses can change to type I responses in situations where local hypoxia is caused (24,25).…”

Section: Discussionmentioning

confidence: 99%

Combination treatment of Cetuximab and photodynamic therapy in SNU-1041 squamous cancer cell line

Kim

Hong²,

Boo³

et al. 2009

Oncol Rep

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Abstract. Cetuximab (Erbitux) has been highlighted for its anti-proliferative effects in solid tumors and it is currently used as an adjuvant modality with other anti-cancer treatments. Photodynamic therapy (PDT) is used widely in many specialties of medicine. This study evaluated the efficacy of a combination treatment of two modalities (Cetuximab, PDT) both in vivo and in vitro. The SNU-1041 cell line was used for both the in vitro and in vivo studies. The in vivo and in vitro experiments were each classified into four groups, control group, Cetuximab applied group, PDT applied group and combined modality group. A migration study was performed to determine the anti-migration effect of Cetuximab, and a MTT assay was performed to compare the anti-proliferative effect of the modalities in vitro. For the in vivo study, the cells were implanted into 5-week-old nude mice. The measured volume of the tumor for each group was compared as a function of time. In the migration study, the control group showed a longer migration length than the Cetuximab applied group. In the MTT assay, the combination modality group showed less survival than the uni-modality groups. The measured tumor size after treatment showed that the combination treatment was more effective than the single modalities. PDT and Cetuximab are treatment modalities that target different molecular pathways. A combination of these two treatment modalities was found to more effective than an individual treatment. However, further studies will be needed to determine the optimal dose of the photosensitizer and Cetuximab.

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“…The incorporation of the photosensitizer in the tissue was confirmed using a fluorescence technique. According to our previous work 8 , where the pharmacokinetics of Photogem ® was investigated in a rat model, we chose a delay time for porphyrin accumulation in the liver of about 30min. This delay time between injection and illumination has been adopted as standard for most of the experiments since.…”

Section: Photodynamic Therapy For the Treatment Of Liver Cancermentioning

confidence: 99%

New photonic technologies for the treatment and diagnosis of hepatic diseases: an overview of the experimental work performed in collaboration, between Physics Institute of São Carlos and Ribeirão Preto Faculty of Medicine of the University of São Paulo

Bagnato¹,

Kurachi²,

Ferreira³

et al. 2006

Acta Cir. Bras.

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Recent advances in optical techniques have created a great range of possibilities for diagnosis and therapeutics in liver related diseases. With the uses of efficient light sources like lasers and LEDs (Light Emitting Diodes) it is possible to employ the light-tissue interaction to promote hepatic tissue regeneration after partial hepatectomy, to detect hepatocarcinoma and steatosis by utilizing optical fluorescence, to evaluate the metabolism of the liver during hepatic transplantation as well as to treat liver tumors. We present here an overview of the technique presently in development at the Ribeirâo Preto Faculty of Medicine - USP in cooperation with the Physics Institute of São Carlos -USP. The results obtained so far have been the subject of a list of publications and are here presented as an overview. A new perspective for modern application of optical techniques in different medical practices related to the liver is presented.

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Pharmacokinetics of Photogem using fluorescence monitoring in Wistar rats

Cited by 42 publications

References 12 publications

Possibility for a full optical determination of photodynamic therapy outcome

Possibility for a full optical determination of photodynamic therapy outcome

Combination treatment of Cetuximab and photodynamic therapy in SNU-1041 squamous cancer cell line

New photonic technologies for the treatment and diagnosis of hepatic diseases: an overview of the experimental work performed in collaboration, between Physics Institute of São Carlos and Ribeirão Preto Faculty of Medicine of the University of São Paulo

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