Pharmacokinetics, optimal dosages and withdrawal time of florfenicol in Asian seabass (Lates calcarifer) after oral administration via medicated feed

Abstract

Asian seabass (barramundi, giant seaperch, Lates calcarifer) is a popular food fish in Asia and Australia. Native to coastal areas of the Indo-West Pacific, it can now be found from the Persian Gulf to China and northern Australia, either in coastal marine, estuarine, or freshwater habitats (Pethiyagoda & Gill, 2012;Rimmer, 2006). The Asian seabass culture technique was first developed in Thailand during the 1970s (Cheong, 1989;Rimmer, 2006). Since then, Asian seabass farming has been expanded and developed to become an economically important species in the Indo-West Pacific region.In 2020, the global production of Asian seabass from aquaculture was 117,446 tonnes. Almost 40% of this production (45,415 tonnes) was from Thailand (FAO, 2022). In Thailand, Asian seabass culture dominates the coastal aquaculture and it is the third place after tilapia (Oreochromis spp.) and clariid catfish (Clarias spp.) as the most

A Green HPLC Approach to Florfenicol Analysis in Pig Urine

“…Analytical methods for determination of the FF and/or its metabolite florfenicol amine (FFA) in blood samples, edible tissues or drug formulations are primarily described in the literature. For example, the following methods were used: voltammetric method [ 13 ], enzyme-linked immunosorbent assay (ELISA) method [ 14 ], ultraviolet-visible (UV-Vis) spectrophotometric method [ 15 ], near-infrared spectroscopy [ 16 ], capillary electrophoresis [ 17 ], and chromatographic methods such as thin-layer chromatography (TLC) [ 18 ], gas chromatography (GC) [ 19 ], (ultra)high-performance liquid chromatography ((U)HPLC) with ultraviolet (UV), diode array detector (DAD) or fluorescence (FL) detector [ 20 , 21 , 22 , 23 , 24 , 25 ], and liquid chromatography-tandem mass spectrometry (LC-MS/MS) [ 26 , 27 , 28 , 29 ]. Only several reported papers included and described the analytical methods for determining the FF in urine samples of animal origin [ 1 , 11 , 12 , 30 , 31 , 32 , 33 ].…”

“…It can also be an alternative sample to edible tissues, for example, for mon antibiotic administration [11] or detecting an appropriate time of animal slaughter (a) (b) Analytical methods for determination of the FF and/or its metabolite flor amine (FFA) in blood samples, edible tissues or drug formulations are primarily des in the literature. For example, the following methods were used: voltammetric m [13], enzyme-linked immunosorbent assay (ELISA) method [14], ultraviolet-visibl Vis) spectrophotometric method [15], near-infrared spectroscopy [16], capillary e phoresis [17], and chromatographic methods such as thin-layer chromatography [18], gas chromatography (GC) [19], (ultra)high-performance liquid chromatog ((U)HPLC) with ultraviolet (UV), diode array detector (DAD) or fluorescence (FL) d [20][21][22][23][24][25], and liquid chromatography-tandem mass spectrometry (LC-MS/MS) [ Only several reported papers included and described the analytical methods for mining the FF in urine samples of animal origin [1,11,12,[30][31][32][33]. However, employ verse and often complex analytical techniques in FF analyses indicated that their on the environment should also be considered.…”