Pharmacological activities of brown seaweed Sargassum wightii (Family Sargassaceae) using different in vitro models

Abstract

Recently a great deal of interest has been developed towards natural bioactive components from seaweeds as functional food ingredients. Antioxidant, anti-inflammatory, antidiabetic and antihypertensive activities of ethyl acetate:methanol (EM) and chloroform solvent fractions (CHCl 3 ) of the brown seaweed Sargassum wightii were evaluated using different in vitro systems. The EM fraction registered greater Fe 2+ ion chelating ability (IC 50 0.52 mg/ml), and were effective in stabilizing the ABTS .+ (IC 50 0.82 mg/ml), and DPPH radicals (IC 50 0.32 mg/ml) than those derived from CHCl 3 fraction. The CHCl 3 fraction showed greater ACE-I inhibitory activity (IC 50 0.084 mg/ml), whilst the EM fraction exhibited greater anti-COX-1, 2, and 5-LOX (IC 50 0.03-0.05 mg/ml) and DPP-4 inhibitory (IC 50 ~ 0.013 mg/ml) properties. A significant colinearity was recorded between target bioactive properties and the electronegative groups Downloaded by [University of Sussex Library] at 04:46 24 June 2016 A c c e p t e d M a n u s c r i p t 2 2 appeared in the downfield space of the NMR spectra of the EM and CHCl 3 solvent fractions from S. wightii.

Antidiabetic Potential of Marine Brown Algae—a Mini Review

“…The results indicated the highest DPP-4 inhibitory activity from ethyl acetate : methanol fraction (IC 50 : 0.013 mg/ml) more than the chloroform fraction (IC 50 : 0.028 mg/ml) compared to the reference drug diprotein-A (IC 50 : 0.007 mg/ml). In addition, the ethyl acetate : methanol (2.51 mg GAE/g) and chloroform (2.04 mg GAE/g) fractions are rich in phenolic compounds which might be attributed to the DPP-4 inhibitory activity [54].…”

Sargassum Seaweed as a Source of Anti-Inflammatory Substances and the Potential Insight of the Tropical Species: A Review

“…Studies on the anti-inflammatory activity of the Sargassum crude sulfated polysaccharides (CSP) have been carried out by several research groups [39,96,97,98,99,100,101,102,103,104,105,106,107] and are shown in Table 2. Sulfated polysaccharides (SP), such as fucoidan, carrageenan, and ulvan, are recognized as the typical functional compounds derived from various types of marine algae.…”

“…The majority of Sargassum crude extracts tested on anti-inflammatory screening are derived from subtropical samples. Seventeen out of 73 studies used samples from tropical regions [34,35,36,39,40,41,42,43,44,45,47,48,49,51,52,53,54]. Most of the observed tropical species came from subgenus Sargassum , including S. polycystum, S. wightii, S. swartzii, S. crassifolium, S. binderi , and S .…”

Exploring bioactive fraction of Sargassum wightii: In vitro elucidation of angiotensin-I-converting enzyme inhibition and antioxidant potential

“…SWE was less explored for its medicinal properties; only few studies have explained the biological activity of the crude extract prepared from S. wightii. [14][15][16] In the present study, S. wightii was fractionated using various solvents in Soxhlet extractor. Further, the solvent fraction was subjected to silica gel chromatography to partially purify and identify the solvent fraction rich in bioactive compounds with both ACE inhibition and antioxidant activity.…”