Pharmacological and antimicrobial studies on different tea-tree oils (Melaleuca alternifolia, Leptospermum scoparium or Manuka and Kunzea ericoides or Kanuka), originating in Australia and New Zealand

Lis‐Balchin, Maria; Hart, Stephen L.; Deans, Stanley G.

doi:10.1002/1099-1573(200012)14:8<623::aid-ptr763>3.0.co;2-z

Cited by 100 publications

(80 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This matches findings of other researchers: aqueous extracts of kinos from 13 Eucalyptus species had activity against the gram-positive bacteria S. aureus and B. subtilis, 13 whilst aqueous extracts of E. olida and E. staigerana also showed activity against S. aureus (MIC 15.6 μg/ml and 125 μg/ml, respectively) although no activity against six other bacteria. 3 The essential oils of M. alternifolia have well documented antibacterial activity against a broad spectrum of bacteria 15,19,24,25 while the activity of aqueous Melaleuca extracts are less well documented. The There have been few investigation into the antibacterial activity of Westringia species.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Activity of Extracts from Native Plants of Temperate Australia

Wigmore¹,

Naiker²,

Bean³

2016

View full text Add to dashboard Cite

Introduction: Significant effort has been invested in looking at the antimicrobial activity of plant extracts from tropical regions of Australia, with less interest in those from more temperate environments. We sought to redress this imbalance by examining antimicrobial activities of extracts from native plants of Victoria. Methods: Sixteen plant samples were obtained around the Ballarat region of Victoria. Plant material was desiccated, ground and extracted with methanol at room temperature. Methanol extracts were subsequently dissolved in water, filtered and freeze dried. Extracts were dissolved in water and their activity determined against eight bacterial species. Plant extracts that showed appreciable antibacterial activity in the initial antimicrobial screen were examined further with both their MICs and MBCs determined. Results: Ten of the sixteen plant extracts showed antimicrobial activity. Extracts of Eucalyptus, Melaleuca, Prostanthera and Westringia were particularly active with MICs as low as 0.25 mg/ml against organisms including P. aeruginosa and S. aureus. Conclusion: The current study demonstrates the antimicrobial activity of plant extracts from temperate Australia. These may serve as precursors for future chemotherapy agents.

show abstract

Section: Discussionmentioning

confidence: 99%

Antimicrobial Activity of Extracts from Native Plants of Temperate Australia

Wigmore¹,

Naiker²,

Bean³

2016

View full text Add to dashboard Cite

show abstract

“…The DPPH scavenging activity of the oil (1%, v/v) in our study was about 14% which is much lower than that of the Kim et al [10] whose data demonstrated that the tea tree oil (1%, v/v) showed 80% activity on DPPH radicals. However, Lis-Balchin et al [11] has demonstrated that essential oil of the Australian tea tree (M. alternifolia) showed no antioxidant activity, while the New Zealand Manuka and Kanuka oils had consistent antioxidant activity. These differences could be due to a difference in chemical constituents of the oils.…”

Section: Free Radical-scavenging Activitymentioning

confidence: 99%

Bioactivity and Chemical Composition of the Essential oil of Tea Tree (Melaleuca alternifolia)

Yang¹,

Jeon²,

Lee³

et al. 2008

Journal of Life Science

View full text Add to dashboard Cite

The essential oil of tea tree (Melaleuca alternifolia) is widely used in traditional Australian medicine for skin lesions and infected injuries. In the present study, we investigated the chemical composition, cytotoxicity and its biological activities. The composition of the oil was analyzed by GC-MS. β-Terpinene (20.87%), α-pinene (17.60%), p-cymene (11.23%), 3-carene (10.40%), trans-anethole (8.47%) and limonene (4.65%) were the major components in the oil. The results tested by MTT assay indicated that the oil showed no cytotoxic effect, at concentrations up to 5%, for less than 3h. The antiradical capacity was evaluated by measuring the scavenging activity of the essential oil on the 2,20-diphenylpicrylhydrazyl (DPPH) and 2,2'-azino-bis 3-ethyl benzothiazoline-6-sulfonic acid (ABTS) radicals. The oil was able to reduce the both radicals dose-dependently, and the concentration required for 50% reduction (RC50) against ABTS radicals (1.6±0.02%) was slightly lower than DPPH radicals (2.6±0.29%). The direct contact and vapor-phase antibacterial activity of the oil were also evaluated using disc diffusion method against Staphylococcus aureus, Streptococcus mutans, Listeria monocytogenes, Acinetobacter baumannii, Escherichia coli, and Vibrio parahaemolyticus. All the Gram-negative bacterial strains tested showed more sensibility to the oil than the Gram-positive strains when compare to the effect of gentamycin. On the other hand, the vapor phase of the essential oil against S. aureus exhibited strongest inhibitory effect.

show abstract

“…Melaleuca alternifolia Cheel é comumente conhecida na Austrália como "árvore de chá", florescendo principalmente em áreas de pântano, próximas de rios 3 . A constituição química do óleo essencial das folhas de M. alternifolia é bem conhecida [3][4][5][6][7] , sendo este rico em terpinen-4-ol, principal responsável por suas propriedades medicinais, principalmente antifúngicas e antibacterianas, garantindo-lhe importância comercial há mais de 60 anos 3 . Embora vários trabalhos relatando a composição química de óleos essenciais de folhas de M. alternifolia sejam encontrados na literatura, nenhum estudo químico de outras partes desta planta foi ainda descrito.…”

Section: Introductionunclassified

“…O presente trabalho relata o primeiro estudo químico do caule e da casca de M. alternifolia, que levou ao isolamento de um derivado metilado do ácido elágico e cinco triterpenos pentacíclicos, dentre eles o ácido 2α,3β,23-triidroxiolean-12-en-28-óico (ácido arjunólico, 2) e o ácido 3β-O-acetilurs-12-en-28-óico (6 O caule (3.250 g) e a casca (700 g), após secagem à temperatura ambiente, foram moídos e submetidos a extrações com hexano (casca) e etanol (casca e caule) em aparelho do tipo Soxhlet. A destilação dos solventes foi realizada sob pressão reduzida em evaporador rotatório, fornecendo 10,5 g de extrato hexânico e 71,0 g de extrato etanólico da casca e 81,0 g de extrato etanólico do caule.…”

Section: Introductionunclassified

Constituintes químicos de Melaleuca alternifolia (Myrtaceae)

et al. 2004

View full text Add to dashboard Cite

Recebido em 2/4/03; aceito em 20/2/04; publicado na web em 27/05/04 CHEMICAL CONSTITUENTS FROM Melaleuca alternifolia (MYRTACEAE). The first chemical study of non-volatile constituents from the bark and stem of Melaleuca alternifolia (Myrtaceae) led to the isolation and identification of 3,3'-Odimethylellagic acid (1) and five pentacyclic triterpenes: 2α,3β,23-trihydroxyolean-12-en-28-oic acid (arjunolic acid, 2), 3β-hydroxylup-20(29)-en-27,28-dioic acid (melaleucic acid, 3), betulinic acid (4), betuline (5), 3β-O-acetylurs-12-en-28-oic acid (6), a mixture of fatty acids and esters, and several hydrocarbons. For 2α,3β,23-trihydroxyolean-12-en-28-oic acid (2) and 3β-Oacetylurs-12-en-28-oic acid (6) a first detailed assignment of 1 H NMR is presented.Keywords: Melaleuca alternifolia; triterpenes; 3,3'-O-dimethylellagic acid. O presente trabalho relata o primeiro estudo químico do caule e da casca de M. alternifolia, que levou ao isolamento de um derivado metilado do ácido elágico e cinco triterpenos pentacíclicos, dentre eles o ácido 2α,3β,23-triidroxiolean-12-en-28-óico (ácido arjunólico, 2) e o ácido 3β-O-acetilurs-12-en-28-óico (6). Para estes dois ácidos foram feitas as atribuições detalhadas dos espectros de RMN de 1 H. Foram também identificados por CG-EM onze outros compostos, além de vários ácidos e ésteres graxos. INTRODUÇÃO PARTE EXPERIMENTAL Instrumentação e procedimentos geraisPara as separações cromatográficas em coluna, foram utilizadas sílicas gel 70 a 230 Mesh e 230 a 400 Mesh. Nas análises por cromatografia em camada delgada (CCD) foram utilizadas placas de sílica gel 60 F 254 , com 0,25 mm de espessura 19 . As temperaturas de fusão, não corrigidas, foram determinadas em um aparelho Microquímica MQAPF-301. Os espectros no Infravermelho (IV) foram registrados em espectrômetro Perkin Elmer, utilizando-se pastilhas de KBr. Os espectros de RMN de 1 H e de 13 C foram obtidos em aparelho Bruker WM 400 (400 MHz), Bruker WM 500 (500 MHz) e Varian Mercury 300 (300 MHz). Os espectros de massas foram registrados em espectrômetro V.G. Analytical ZAB-IF a 70 eV. As análises dos ácidos graxos e triacilglicerídeos por cromatografia em fase gasosa foram realizadas em cromatógrafo Shimadzu GC 17-A, acoplado a um registrador C-R7A Chromatopac. As análises por CG-EM foram realizadas em cromatógrafo Shimadzu GC 17-A, com detector seletivo de massas, modelo QP 5050 (Shimadzu). Material vegetal, extração e isolamento das substânciasO caule e a casca de M. alternifolia Cheel foram coletados no Setor de Dendrologia do Departamento de Engenharia Florestal da Universidade Federal de Viçosa. A exsicata correspondente ao material coletado foi depositada no Herbário VIC, da mesma Instituição, sob o número 26046.O caule (3.250 g) e a casca (700 g), após secagem à temperatura ambiente, foram moídos e submetidos a extrações com hexano (casca) e etanol (casca e caule) em aparelho do tipo Soxhlet. A destilação dos solventes foi realizada sob pressão reduzida em evaporador rotatório, fornecendo 10,5 g de extrato hexânico e 71,0 g d...

show abstract

Pharmacological and antimicrobial studies on different tea-tree oils (Melaleuca alternifolia, Leptospermum scoparium or Manuka and Kunzea ericoides or Kanuka), originating in Australia and New Zealand

Cited by 100 publications

References 20 publications

Antimicrobial Activity of Extracts from Native Plants of Temperate Australia

Antimicrobial Activity of Extracts from Native Plants of Temperate Australia

Bioactivity and Chemical Composition of the Essential oil of Tea Tree (Melaleuca alternifolia)

Constituintes químicos de Melaleuca alternifolia (Myrtaceae)

Contact Info

Product

Resources

About