Pharmacological characterisation of apical Na+ and Cl– transport mechanisms of the anal papillae in the larval mosquito<i>Aedes aegypti</i>

Duca, Olimpia Del; Nasirian, Azadeh; Galperin, Vladimir; Donini, Andrew

doi:10.1242/jeb.063719

Cited by 30 publications

(13 citation statements)

References 41 publications

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“…This acidification of the hemolymph was shown to occur in A. aegypti larvae that were transferred to relatively high salt levels (Donini et al, 2007). Evidence that Na + uptake is associated with H + secretion in A. aegypti has been presented (Patrick et al, 2002b;Stobbart, 1967) and we have shown that Na + uptake at the papillae is likely to occur through a putative Na + channel driven by the electrical gradient established by the apical VA, which secretes H + into the water (Del Duca et al, 2011). We have since shown the expression of NHE3 on the apical side of the anal papilla epithelium and cannot rule out the possibility of Na + uptake coupled to H + secretion via this transporter (Chasiotis et al, 2016).…”

Section: Nacl Rearingsupporting

confidence: 51%

Evidence that Rh proteins in the anal papillae of the freshwater mosquitoAedes aegyptiare involved in the regulation of acid base balance in elevated salt and ammonia environments

Durant

Donini

2018

Journal of Experimental Biology

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Aedes aegypti commonly inhabit ammonia-rich sewage effluents in tropical regions of the world where the adults are responsible for the spread of disease. Studies have shown the importance of the anal papillae of A. aegypti in ion uptake and ammonia excretion. The anal papillae express ammonia transporters and Rhesus (Rh) proteins which are involved in ammonia excretion and studies have primarily focused on understanding these mechanisms in freshwater. In this study, effects of rearing larvae in salt (5 mmol l −1 NaCl) or ammonia (5 mmol l −1 NH 4 Cl) on physiological endpoints of ammonia and ion regulation were assessed. In anal papillae of NaCl-reared larvae, Rh protein expression increased, NHE3 transcript abundance decreased and NH 4 + excretion increased, and this coincided with decreased hemolymph [NH 4 + ] and pH. We propose that under these conditions, larvae excrete more NH 4 + through Rh proteins as a means of eliminating acid from the hemolymph. In anal papillae of NH 4 Clreared larvae, expression of an apical ammonia transporter and the Rh proteins decreased, the activities of NKA and VA decreased and increased, respectively, and this coincided with hemolymph acidification. The results present evidence for a role of Rh proteins in acid-base balance in response to elevated levels of salt, whereby ammonia is excreted as an acid equivalent.

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Section: Nacl Rearingsupporting

confidence: 51%

Evidence that Rh proteins in the anal papillae of the freshwater mosquitoAedes aegyptiare involved in the regulation of acid base balance in elevated salt and ammonia environments

Durant

Donini

2018

Journal of Experimental Biology

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“…In larval Ae . aegypti , four anal gills surrounding the anal opening serve as the major sites for Na + , Cl − and K + uptake by H + -ATPase and Na + /K + -ATPase 37,69,70 . The osmotic uptake of water at the anal gills is the primary external site of ion uptake, normally contributing to 33% of the body weight gain per day 70 .…”

Section: Discussionmentioning

confidence: 99%

Toxicity and possible mechanisms of action of honokiol from Magnolia denudata seeds against four mosquito species

Wang

Perumalsamy

Xue

et al. 2019

Sci Rep

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This study was performed to determine the toxicity and possible mechanism of the larvicidal action of honokiol, extracted from Magnolia denudata seeds, and its 10 related compounds against third-instar larvae of insecticide-susceptible Culex pipiens pallens, Aedes aegypti, and Aedes albopictus and Anopheles sinensis resistant to deltamethrin and temephos. Honokiol (LC50, 6.13–7.37 mg/L) was highly effective against larvae of all of the four mosquito species, although the toxicity of the compound was lower than that of the synthetic larvicide temephos. Structure–activity relationship analyses indicated that electron donor and/or bulky groups at the ortho or para positions of the phenol were required for toxicity. Honokiol moderately inhibited acetylcholinesterase and caused a considerable increase in cyclic AMP levels, indicating that it might act on both acetylcholinesterase and octopaminergic receptors. Microscopy analysis clearly indicated that honokiol was mainly targeted to the midgut epithelium and anal gills, resulting in variably dramatic degenerative responses of the midgut through sequential epithelial disorganization. Honokiol did not affect the AeCS1 mRNA expression level in Ae. aegypti larvae, but did enhance expression of the genes encoding vacuolar-type H+-ATPase and aquaporin 4, indicating that it may disturb the Na+, Cl− and K+ co-transport systems. These results demonstrate that honokiol merits further study as a potential larvicide, with a specific target site, and as a lead molecule for the control of mosquito populations.

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“…The anal gills of mosquito larvae are important primary sites of NaCl uptake, thereby acting to offset the dilution of the hemolymph by the dilute habitat [21] , [43] , [44] . Donini and O'Donnell [22] confirmed with the use of self-referencing ion-selective microelectrodes that the anal gills of Ae.…”

Section: Discussionmentioning

confidence: 99%

Novel Histopathological and Molecular Effects of Natural Compound Pellitorine on Larval Midgut Epithelium and Anal Gills of Aedes aegypti

Perumalsamy

Kim

et al. 2013

PLoS ONE

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The yellow fever mosquito, Aedes aegypti, is a vector for transmitting dengue fever and yellow fever. In this study, we assessed the histopathological and molecular effects of pellitorine, an isobutylamide alkaloid, on the third instar of Ae. aegypti larvae. At 5 mg/l concentration of pellitorine, the whole body of the treated larvae became dark in color, particularly damaged thorax and abdominal regions. Pellitorine was targeted mainly on midgut epithelium and anal gills, indicating variably dramatic degenerative responses of the midgut through a sequential epithelial disorganization. The anterior and posterior midgut was entirely necrosed, bearing only gut lumen residues inside the peritrophic membranes. Pellitorine caused comprehensive damage of anal gill cells and branches of tracheole and debris was found in hemolymph of the anal gills. RT-PCR analysis indicates that the compound inhibited gene expression encoding V-type H+-ATPase and aquaporine 4 after treatment with 2.21 mg/l pellitorine. These results verify that pellitorine merits further study as a potential larvicide with a specific target site and a lead molecule for the control of mosquito populations.

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Pharmacological characterisation of apical Na+ and Cl– transport mechanisms of the anal papillae in the larval mosquitoAedes aegypti

Cited by 30 publications

References 41 publications

Evidence that Rh proteins in the anal papillae of the freshwater mosquitoAedes aegyptiare involved in the regulation of acid base balance in elevated salt and ammonia environments

Evidence that Rh proteins in the anal papillae of the freshwater mosquitoAedes aegyptiare involved in the regulation of acid base balance in elevated salt and ammonia environments

Toxicity and possible mechanisms of action of honokiol from Magnolia denudata seeds against four mosquito species

Novel Histopathological and Molecular Effects of Natural Compound Pellitorine on Larval Midgut Epithelium and Anal Gills of Aedes aegypti

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